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V Kubícek

Publications and source records attributed to V Kubícek.

At least 19 recordsLinked to original sources
PubMed2005

[Use of dinitrophenols in ion-pair extraction spectrophotometry of cationic tensides].

The application of 2,4-dinitrophenol and 2,6-dinitrophenol as reagents for a ion pair extraction spectrophotometry was tested. The ion pair extraction spectrophotometry experiments were carried out using a simplified procedure (test tube method). Four cationic tensides were used as model analytes. After the optimal conditions of the assay were being determined, the influence of the concentration excess of the reagents on the absorbance of the chloroform extract was investigated. Calibration curves were constructed; an estimation of limits of quantitation was performed and apparent molar absorption coefficients were determined. The obtained results show that both investigated dinitrophenols are suitable for the studied purpose.

Dinitrophenols↗
PubMed2002

[The present status of andrology in the Czech Republic].

Andrology is defined as a branch of science and medicine dealing with the male reproductive organs in animals and in men and with the diseases of these organs. Included in this definition are all related specialized branches of science (Definition of the International Society of Andrology). This definition is very wide for the perspectives of andrology it is not optimal. In Czech Republic the Andrological Section of the Czech Urological Society was founded in March 1998. This section aims to disseminate knowledge on andrology by coordination and organization of congresses, conferences and courses in andrology, to encourage the development of basic and clinical research in andrology, to facilitate the collaboration by distribution information and to encourage sponsoring of publication related to the field of andrology.

Czech Republic↗
PubMed2000

[Erectile dysfunction--present possibilities of treatment].

Paper brings the basic information in pathophysiology, diagnostics and differential diagnostics in causes of the erectile dysfunction and provides an overview of the contemporary therapeutic possibilities, including the new substance--sildenafil citrate (VIAGRA). It is stressed that sufficient knowledge and responsibility in the indication of therapy may highly support positive contribution of the erectile dysfunction and differentiate patients of the potential risk. At present, adequate form of treatment of erectile and sexual dysfunction exists for almost every patient. New remedies have broaden possibilities of treatment, however, they have not done the indication or therapy easier.

Erectile Dysfunction↗
PubMed2000

Effect of substituents on microsomal reduction of benzo(c)fluorene N-oxides.

The potential benzo(c)fluorene antineoplastic agent benfluron (B) displays high activity against a broad spectrum of experimental tumours in vitro and in vivo. In order to suppress some of its undesirable properties, its structure has been modified. Benfluron N-oxide (B N-oxide) is one of benfluron derivatives tested. The main metabolic pathway of B N-oxide is its reduction to tertiary amine B. A key role of cytochrome P4502B and P4502E1 in B N-oxide reduction has been proposed in the rat. Surprisingly, B N-oxide is reduced also in the presence of oxygen although all other N-oxides undergo reduction only under anaerobic conditions. With the aim to determine the influence of the N-oxide chemical structure and its redox potential on reductase affinity, activity and oxygen sensitivity five relative benzo(c)fluorene N-oxides were prepared. A correlation between the redox potential measured and the non-enzymatic reduction ability of the substrate was found, but no effect of the redox potential on reductase activity was observed. Microsomal reductases display a high affinity to B N-oxide (apparent K(m) congruent with0. 2 mM). A modification of the side-chain or nitrogen substituents has led to only a little change in apparent K(m) values, but a methoxy group substitution on the benzo(c)fluorene moiety induced a significant K(m) increase (ten-fold). Based on kinetic study results, the scheme of mechanism of cytochrome P450 mediated benzo(c)fluorene N-oxides reduction have been proposed. All benzo(c)fluorene N-oxides under study were able to be reduced in the presence of oxygen. Changes in the B N-oxide structure caused an extent of anaerobic conditions preference. The relationship between the benzo(c)fluorene N-oxide structure and the profile of metabolites in microsomal incubation was studied and important differences in the formation of individual N-oxide metabolites were found.

Aerobiosis↗
PubMed2000

[Determination of cetrimide and chlorhexidine diiodide in microparticles of a biodegradable copolymer based on lactic acid].

The procedure of the determination of cetrimide and chlorohexidine diiodide in polymer microparticles was proposed and verified. The described procedure combines the spectrophotometric method to determine chlorohexidine diiodide and the non-aqueous titration to determine the sum of nitrogen bases. To verify the ability of the proposed procedure to yield reliable results, the determinations of repeatability and accuracy were performed. The results confirmed the suitability of the proposed procedure for the indicated purpose. Using the procedure, samples were successfully analysed during the development of the new antiseptic powder containing antiseptic drugs in a biodegradable polymer.

Cetrimonium↗
PubMed1990

[An unusual complication of myxoma of the left atrium].

The authors give an account of a 59-year-old female patient with a myxoma of the left atrium which was complicated by repeated multiple embolizations into the systemic circulation. The cause of death after a successful operation was haemorrhage into the abdominal cavity from a ruptured spleen, as a result of a new lienal infarction after embolization of a portion of the myxoma into the lienal artery, 24 before the operation. The authors emphasize the necessity of immediate removal of an intracardiac formation as soon as it is detected.

Female↗