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Biomedical subjects

V L Jacobs

Publications and source records attributed to V L Jacobs.

9 recordsLinked to original sources

Charge-exchange-induced two-electron satellite transitions from autoionizing levels in dense plasmas.

Order-of-magnitude anomalously high intensities for two-electron (dielectronic) satellite transitions, originating from the He-like 2s(2) 1S0 and Li-like 1s2s(2) (2)S(1/2) autoionizing states of silicon, have been observed in dense laser-produced plasmas at different laboratories. Spatially resolved, high-resolution spectra and plasma images show that these effects are correlated with an intense emission of the He-like 1s3p 1P-1s(2) 1S lines, as well as the K(alpha) lines. A time-dependent, collisional-radiative model, allowing for non-Maxwellian electron-energy distributions, has been developed for the determination of the relevant nonequilibrium level populations of the silicon ions, and a detailed analysis of the experimental data has been carried out. Taking into account electron density and temperature variations, plasma optical-depth effects, and hot-electron distributions, the spectral simulations are found to be not in agreement with the observations. We propose that highly stripped target ions (e.g., bare nuclei or H-like 1s ground-state ions) are transported into the dense, cold plasma (predominantly consisting of L- and M-shell ions) near the target surface and undergo single- and double-electron charge-transfer processes. The spectral simulations indicate that, in dense and optically thick plasmas, these charge-transfer processes may lead to an enhancement of the intensities of the two-electron transitions by up to a factor of 10 relative to those of the other emission lines, in agreement with the spectral observations.

Journal Article↗

Influence of acute intracerebroventricular (i.c.v.) administration of adrenocorticotrophin (ACTH) on LH secretion in male rats: effect of pretreatment (i.c.v.) with ACTH antiserum on the serum LH response to an acute ether stress.

Adult male rats with chronic indwelling intracerebroventricular (i.c.v.) and jugular catheters were given an i.c.v. injection (over 1 min) of 1, 10, 100 ng or 1 microgram ACTH(1-24), or 300 ng ACTH(4-10) or saline, and blood samples were taken 0, 5, 15, 30 and 60 min later. Increasing dosages of ACTH(1-24) caused a dose-related rise in serum LH levels. Peak levels of serum LH (ranging from 157 to 473% of pretreatment levels) were reached 5-15 min after treatment, and then serum LH values returned to pretreatment levels by 60 min. The serum LH response to 1 microgram ACTH(1-24) did not differ from the response to 100 ng ACTH(1-24). Administration (i.c.v.) of 300 ng ACTH(4-10) was also effective in increasing serum LH values. Repeated withdrawal of blood during the experiment increased serum corticosterone values in all groups (including saline-treated), but i.c.v. administration of ACTH(1-24) or ACTH(4-10) did not further increase serum corticosterone levels. Two additional groups of rats were injected i.p. with either saline or pentobarbital (30 mg/kg body weight) 1 h before i.c.v. administration of 10 ng ACTH(1-24) and blood samples were taken as in the other groups. The animals in these groups did not show a rise in serum LH concentrations in response to ACTH(1-24). In a third experiment, rats were pretreated (i.c.v.) with either ACTH antiserum (ACTH-Ab) or normal rabbit serum 15 min before a 2-min ether stress. The ether stress evoked a significant rise in serum LH concentrations within 15 min.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenocorticotropic Hormone↗

Experience with a cost-effective crossmatch protocol.

Pretransfusion blood samples were routinely tested for ABO group, Rh type, and the presence of unexpected red blood cell antibodies. Patients who had unexpected red blood cell antibodies received transfusions of blood that was crossmatched using an immediate spin test, a 37 degrees C incubation step, and an indirect antiglobulin test. Patients who did not have unexpected red blood cell antibodies received transfusions with blood that was crossmatched by an immediate spin crossmatch only. Because the average immediate spin crossmatch required only 3.25 minutes to be performed, crossmatches were not done for patients without unexpected antibodies until blood was actually requested to be issued for transfusion. During the first 8 1/2 months this protocol was used, 27,742 crossmatches were performed and 46,959 unnecessary crossmatches were avoided, thus reducing direct costs by at least +49,300. This protocol also allowed for optimal blood inventory control and minimized the outdating of units of blood to only 0.19%.

Blood Banks↗

Structures of the bovine vomeronasal complex and its relationships to the palate: tongue manipulation.

The rostral part of the bovine vomeronasal complex was examined to determine its relation structures of the palate which are compressed by the tongue during investigative sexual behavior. The rostral vomeronasal cartilage (VNC) is C-shaped and contains the incisive duct (ID). The hilus of the cartilage is directed medially, approximately 0.5 cm dorsal to the palate. Just caudal to the origin of the vomeronasal duct, the VCN lies approximately 1 cm above the palate with a ventrally oriented hilus containing the ID. Further caudally, the hilus is directed laterally, toward the nasal cavity. Palatal mucosa occupies the intervomeronasal, cartilaginous area below the nasal septum and caudal to the incisive papilla. This mucosa and the ID appear to be compressed by the bull's everted tongue, forcing fluid samples into the vomeronasal system.

Animals↗

Ascending projections of the dorsal column in a garter snake (Thamnophis siritalis): a degeneration study.

Ascending axons in the dorsal column of garter snakes were examined following hemisection of the spinal cord at segment levels 2, 3, 4, 11, 13, and 31. After postoperative survival periods of 11 to 28 days, sections of the spinal cord and brain were processed with a silver method to demonstrate degenerated axons and preterminals. The study demonstrated that most ascending degenerated axons are located in the outer half of the dorsal column. The somatotopic pattern of ascending fibers is evident, whereby dorsomedial fibers are primarily of caudal origin and the more dorsolateral axons are from rostral cord segments. Rostral to segment 31, all spinal segments appear to project to a strip of dorsal column adjacent to the dorsal median septum. From the septum, axons descend to terminate somatotopically on cells of the nucleus of Bischoff located caudal to the obex of the medulla. Dorsal column degeneration ascends to the level of the dorsal column nuclei, where most fibers terminate. Degeneration from caudal cord segments terminates on caudo-medial cells of the dorsal column nuclei, while rostral cord segments project to rostro-lateral cells. The dorsal column nuclei consist of an expanded lateral part between tractus descendens trigemini and the vago-solitary complex, and a contiguous, thin medial lamina of cells dorsal and medial to the vagal nuclei. The somatotopic pattern of degeneration in the dorsal column nuclei, probably of dorsal root origin, follows the mammalian organization, which suggests that the garter snake has primitive nuclei gracilis and cuneatus. Other terminal sites of degenerating fibers, although probably of spinal gray origin, are nucleus commissura infima, nucleus descendens vestibuli, and area postrema.

Animals↗

Tongue manipulation of the palate assists estrous detection in the bovine.

To understand the mechanisms for introducing urine or vaginal secretions into the vomeronasal organ, we used 16 mm cinematography and a freeze frame/slow motion technique to analyze the mouth and tongue movements of Brahman bulls while they examined the vulvas of restrained, estrogen-primed cows. Prior to flehmen, the mouth slowly opened, the curled tip of the tongue compressed the hard palate and the body of the tongue protruded from the mouth. The tongue maintained this form and moved forward. Once the tip of the tongue reached the incisive papilla, the body of the tongue retracted and the tip of the tongue relaxed. This tongue compression stroke (TCS) of the hard palate occurred 2 to 6 times, lasting 1 4 to 1 2 sec/stroke. Pressure changes in the vomeronasal organ are assumed to occur during and following TCSs, resulting in aspiration of any liquid in the incisive pit into the incisive and vomeronasal ducts. Such aspiration probably does not occur during flehmen because the tongue is relaxed and on the floor of the mouth.

Journal Article↗

The sensory component of the facial nerve of a reptile (Lacerta viridis).

The sensory fibers of the facial nerve in Lacerta viridis have been studied with a silver impregnation method to follow the course of axonal degeneration. Destruction of the geniculate ganglion demonstrated the degenerated sensory component of the facial nerve adjacent to the anterior vestibular root. Within the lateral vestibular area the facial sensory fibers consist of numerous rootlets separated by vestibular fibers and cells. These rootlets may join to form a main or paired sensory tract that passes through the vestibular nuclei to enter the tractus solitarius and divide into a small ascending prefacial component and a major descending prevagal division. A few fibers continue into the postvagal part of tractus solitarius and extend caudally to terminate in the nucleus commissura infima. Prefacial fibers terminate along the periventricular gray while prevagal fibers terminate within the tractus solitarius on the dendrites of cells of nucleus tractus solitarius and near the periphery of the dorsal motor nucleus of X. There was no noticeable degeneration in the descendens tractus trigemini. Terminal degeneration to descendens nucleus trigemini and motor nucleus of VII followed the tractus solitarius course. Most facial sensory fibers are probably related to taste and other visceral information.

Animals↗