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Biomedical subjects

V L Sutter

Publications and source records attributed to V L Sutter.

At least 73 records · Page 4Linked to original sources
PubMed1974

Susceptibility of Clostridium ramosum to antimicrobial agents.

The susceptibility of 49 strains of Clostridium ramosum to 10 antibiotics was determined by agar dilution and disk diffusion tests. Results showed that, among the anaerobes, C. ramosum is second only to Bacteroides fragilis in its resistance to antimicrobial agents. All strains were susceptible to penicillin, carbenicillin, chloramphenicol, vancomycin, and metronidazole at readily achievable blood levels. Most strains (83%) were susceptible to erythromycin. There was a high level of resistance to clindamycin in 16% of the strains. All isolates were resistant to rifampin and gentamicin, and most were resistant to lincomycin. Assessment of susceptibility by measurement of inhibition zone diameters with disk diffusion tests was not satisfactory.

Anti-Bacterial Agents↗
PubMed1973

Susceptibility of Bacteroides fragilis to six antibiotics determined by standardized antimicrobial disc susceptibility testing.

The susceptibility of 100 or more strains of Bacteroides fragilis to six antibiotics was determined by standardized agar dilution and disc diffusion tests. Good correlation of results of the two methods was obtained with chloramphenicol, clindamycin, lincomycin, penicillin, and vancomycin. Correlation of results with erythromycin was not as good. A great deal of overlapping of zone diameters among strains classed as susceptible, intermediate, and resistant occurred with both erythromycin and lincomycin, making interpretation of disc diffusion tests difficult. All strains tested were susceptible to chloramphenicol; 94% were susceptible to clindamycin at concentrations readily achieved with ordinary dosage, and all strains were inhibited by 6.2 mug/ml, a level which is achieved with somewhat more intensive therapy. Only 7% were shown to be susceptible to erythromycin and 13% to lincomycin under the conditions of our testing procedure. Only a small percentage of the strains were susceptible to penicillin, and none was susceptible to vancomycin.

Anti-Bacterial Agents↗
PubMed1972

Standardized antimicrobial disc susceptibility testing of anaerobic bacteria: in vitro susceptibility of Clostridium perfringens to nine antibiotics.

The in vitro susceptibility of 43 strains of Clostridium perfringens to nine antibiotics was determined by a standardized test for rapid-growing anaerobes. Good correlation was established between the agar dilution susceptibility and the disc diffusion susceptibility results. The inhibition zone diameters around the antibiotic discs, however, were generally much smaller than those of gram-negative anaerobes previously studied. All of the strains tested were susceptible in vitro to chloramphenicol, clindamycin, doxycycline, minocycline, penicillin, and vancomycin. Erythromycin showed poor in vitro activity against this organism, with only 7% of the strains susceptible, 72% intermediate in susceptibility, and 21% resistant. In tests of the 43 strains against lincomycin, 58% were susceptible, 32.5% were intermediate in susceptibility, and 9.5% were resistant. Against tetracycline, 37% of the strains were intermediate in susceptibility and the rest were susceptible.

Anaerobiosis↗
PubMed1972

Standardized antimicrobial disc susceptibility testing of anaerobic bacteria. I. Susceptibility of Bacteroides fragilis to tetracycline.

A modified Bauer-Kirby-Sherris-Turck method for disc susceptibility testing of anaerobic bacteria is presented. When tetracycline was used against 100 strains of Bacteroides fragilis as a model, reasonably reproducible results were obtained after overnight incubation in both the GasPak atmosphere and an atmosphere achieved by adding 10% CO(2) to a mixture of 10% H(2) and 90% N(2). The minimal inhibitory concentration for the strains determined by the agar dilution technique correlated well with the results of disc tests performed in the GasPak atmosphere with 30-mug tetracycline discs. Among 63 strains isolated from 1970 to the present, only 24 (38.1%) were found to be susceptible to tetracycline.

Anaerobiosis↗
PubMed1972

Bacteremia after genitourinary tract manipulation: bacteriological aspects and evaluation of various blood culture systems.

A total of 300 patients undergoing various types of urological procedures was studied for incidence of bacteremia. An osmotically stabilized anaerobic broth with sodium polyanethol sulfonate (Liquoid) yielded more positive blood cultures than any other culture system and was also the best system by far for recovery of anaerobes. The membrane filter showed faster growth and, therefore, facilitated faster identification of the infecting organism. There was a 31% incidence of bacteremia in the patients having transurethral resection of the prostate, 17% in the cystoscopy group, 24% in the urethral dilation group, and 8% in the urethral catheterization group. The organisms found most frequently isolated in blood cultures were enterococci and Klebsiella pneumoniae. Notable were a relatively large number of anaerobes and two protoplasts. The major source of the bacteremia was previous urinary tract infection, but evidence is presented which indicates that the prostate gland and the normal urethral flora are other significant sources.

Anaerobiosis↗
PubMed1972

Metronidazole versus anaerobes. In vitro data and initial clinical observations.

Metronidazole, a systemic trichomonicide which has been used extensively since 1960, has recently been shown to be active against various anaerobic bacteria in vitro and in experimental infections. In the present study, metronidazole showed significant activity against virtually all of 54 strains of anaerobic and microaerophilic bacteria tested, including Bacteroides fragilis. Since this organism is the anaerobe most commonly isolated from human infections and has demonstrated significant resistance to many antimicrobial agents, metronidazole may prove to be very useful. Our initial clinical evaluation of metronidazole in human anaerobic infections is presented. Because of metronidazole's in vitro activity, its low incidence of toxic reactions, and this initial favorable clinical trial, the drug deserves further evaluation in the management of anaerobic infections.

Adult↗
PubMed1971

Antibiotic disc susceptibility tests for rapid presumptive identification of Gram-negative anaerobic bacilli.

A method for identification of gram-negative anaerobic bacilli is described. Based on differences in susceptibility to paper discs containing 10 mug of colistin, 60 mug of erythromycin, 1,000 mug of kanamycin, 1,000 mug of neomycin, 2 units of penicillin, and 15 mug of rifampin, these bacteria may be placed into five groups. Other tests such as colony morphology, production of pigment, growth in bile, esculin hydrolysis, and reaction on egg yolk-agar may be used for further identification. The susceptibility tests are rapid and simple to perform and are helpful in characterizing gram-negative anaerobic bacilli. They are not intended for use in predicting clinical effectiveness of the drugs utilized.

Anti-Bacterial Agents↗