[Effect of the geroprotector 2-ethyl-6-methyl-3-hydroxypyridine chlorhydrate on the cell proliferation of Acholeplasma laidlawii].
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Biomedical subjects
Publications and source records attributed to V L Ushakov.
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Under observation were 23 patients with leiomyomas of the stomach. Leiomyomas were more frequently observed in females, in the proximal segment these were as frequent as in the distal one, but more frequently in the posterior wall. Submucous endo gastric localization was typical for most leiomyomas. The possibilities of clinico-roentgenological diagnosis were studied. The clinical picture of leiomyomas of the stomach shows pains in the epigastric region, general disturbances, acute profuse gastric hemorrhages. The most typical roentgenological sign of gastric leiomyoma is an oval or round filling defect with even margins around 5-6 cm in size. Not infrequently there are one or several ulcerations on the leiomyoma surface like "a niche" of oval or semioval shape. The mucosa folds around the filling defect are moved apart but not destroyed. Some information on gastroscopic examination of 8 patients with gastric leiomyomas is reported.
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The sensitivity of the anomalous time dependence of viscosity to the concentration of the DNA-protein complexes (DNA + histone-like proteins of bacteria or, in other words, the genome) such as chromatin and the conformations of these complexes in lysates of E. coli AB1157 cells were studied. A linear region of the anomalous viscosity time dependence on the concentration of E. coli cells was found in which the interactions between single DNA-protein complexes can be neglected. The response of the genome of E. coli to ethidium bromide at concentrations of 0.0003-3 mg/ml was studied. Significant differences in the effect of ethidium bromide on E. coli cells in the stationary and logarithmic growth phases were found. The effect of heating cell lysates, the molar concentration of NaCl in lysates, and the addition of proteins into lysates on the parameters of the anomalous viscosity time dependence was studied. It was shown that proteins do not contribute significantly to the effect of anomalous viscosity time dependence. The results obtained confirm that the method is sensitive to changes in the conformational state of the genome of E. coli cells.
In 12 healthy subjects and 9 schizophrenic patients in the background conditions (with eyes closed) EEG was recorded from 16 standard derivations (10-20 system) during 3 min. The record underwent the spectral analysis detecting alpha- and theta-frequency bands. After the preliminary narrow band filtration for the main frequencies the sources of the spontaneous rhythms were localized. The data on localization for all healthy subjects and patients were summarized. The K-means clustering was used for identification of the sources clusters which were revealed in occipital and parietal lobes and limbic cortex for alpha-rhythm and also in frontal, temporal and parietal regions, limbic cortex and hippocampus for theta-rhythm. In schizophrenic patients in comparison with healthy subjects there was revealed significant increase of the numbers of dipole sources of alpha-rhythm in the clusters localized in limbic cortex and hippocampus. For theta-rhythm there was significant increase of the dipole moment of the sources in the clusters localized in the temporal and frontal cortices and hippocampus in patients in comparison with the norm.
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