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Biomedical subjects

V L Virnik

Publications and source records attributed to V L Virnik.

6 recordsLinked to original sources
PubMed1985

[Lanthanum-induced damage to microvilli in cells of the posterior epithelium of the cornea and its trigger mechanism].

Destructions in filament-membranous connections of microvilli of cells of the rabbit corneal posterior epithelium have been investigated after the effect of an ionized form of lanthanum. Rare-earth ions are used as a specific antagonist and as a substituent for calcium on the surface of biopolymeres. Twelve corneas from 6 rabbits of Shinshilla strain at the age of 2 months are used. Lanthanum ions are stated to have selective connections with the luminal part of the plasmolemma and with the membrane of the microvilli in the cells of the corneal posterior epithelium. The changes in the microvilli are accompanied with destruction of the filament-membranous connections producing protrusions and vesiculation of their plasmolemma, disaggregation of microfilaments adjoining the luminal part of the cellular plasmolemma. A suggestion is made on an impostant role of calcium and C++-binding biopolymeres in the mechanism regulating the filament-membranous connections.

Adenosine Triphosphate↗
PubMed1985

[Pathways of lanthanum ion transport across the posterior epithelium of the cornea in rabbits].

Morphological estimation of the barrier-transport properties of the posterior epithelium in the donor cornea has been performed in the most early time of fanoxic lesions of the cells. Ionized lanthanum, as an effective inhibitor of oxidative phosphorylation and simultaneously--as a marker of transport pathways for particles similar in their size to water molecule, is used in the investigation. The concentration gradient of rare-earth ions is produced by vital injection of lanthanum trichloride into the proper substance (stroma) of the cornea. From the microinjection focus the electron opaque marker is transported through the substrate of the basal substance of the stroma to the posterior epithelium and further along its intercellular and transcellular pathways into the anterior chamber of the eye. The rare-earth ions freely penetrate through the gap and tight junctions. Transcellular transport of lanthanum in the contents of the plasmolemmal microvesicles, vital absorption of the marker on the lateral and luminal parts of the plasmolemma and on the intracellular membranes, lesions of mitochondria and canaliculi of the endoplasmic reticulum of the anoxic character are stated. A suggestion is made on structural preservation of the pathways of convective and dissipative transition of the substance through the posterior epithelium of the cornea during the earliest time of the experimental anoxia.

Animals↗