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Biomedical subjects

V Lackovic

Publications and source records attributed to V Lackovic.

At least 37 records · Page 2Linked to original sources
PubMed1980

Production of interferon and other lymphokines during murine tumour growth. I. Lymphokines in cell--free fluid of rat Zajdela ascites hepatoma.

Cell-free ascites of rat Zajdela hepatoma was assayed for the presence of lymphokine-like factors. Macrophage migration inhibitory and microbial growth inhibitory cytokines were detected with peak activities at day 4-8 and 10-11 after tumour inoculation, respectively. Occasionally, skin reactive activity was found, whereas only borderline titres of an interferon-like substance were demonstrated. Preliminary studies indicated that both migration inhibitory and microbial growth inhibitory factors are proteins resembling the corresponding lymphocyte-derived lymphokines. The cellular site of formation of these factors remains to be determined.

Animals↗
PubMed1980

Production of interferon and other lymphokines during murine tumour growth. II. Induction of an interferon by Ehrlich ascites cells in outbred mice.

A viral inhibitor produced in the peritoneal cavity of outbred Swiss mice inoculated with allogeneic Ehrlich ascites cells was identified as an interferon. It was antigenically related to viral interferons of type 1 and seems to be produced by macrophages. The interferon activity in the peritoneum reached maximal levels at 24 hr after tumour challenge. Interferon induction by Ehrlich ascites cells was not inhibited in lipopolysaccharide-hyporeactive mice. In addition, cell growth inhibition, enhanced interferon production after priming with small doses of this interferon, and increased susceptibility of cells treated with this interferon to the toxic effect of double-stranded RNA was demonstrated.

Animals↗
PubMed1979

Production of antiviral and migration inhibitory activities in the peritoneum of mice after inoculation with allogenic Ehrlich ascites tumor cells.

Peritoneal inoculation of Ehrlich ascites cells into mice stimulates the appearance of migration inhibitory factor (MIF) not only in the peritoneum but also in the circulation of mice. Whereas the MIF-activity in the peritoneum reached maximum at 6 days and disappeared within 17 days, the MIF-activity of sera reached maximum levels at about 11 days after intraperitoneal inoculation of tumor cells (10(6) of EAC per mouse). In addition, an antiviral activity of peritoneal exudates was observed in the early stage (24--72 h) of tumor challenge. The time-course of appearance of both MIF and AV-inhibitor are presented. A significant decrease of MIF-activity was noted when the tumor-inoculated mice were treated with ds-RNA, antithymocyte serum and cyclophosphamide, respectively. The appearance of antiviral activity in the peritoneum of tumor-inoculated mice seems to be macrophage-dependent. Although the antiviral factor meets several criteria of interferons, further studies are required for its characterization.

Animals↗
PubMed1979

Long-term culture of guinea pig tongue cells: a suitable interferon system.

The interferon system was investigated in a long-term culture of guinea pig tongue (GPT) cells. Interferon synthesis was induced with Newcastle disease virus (B strain) and f2 phage double-stranded RNA. Guinea pig interferons were about 2 times more active in cell cultures established from guinea pig embryos than in the GPT cells. Human leukocyte and mouse peritoneal cell interferons were active in this cell system also.

Animals↗
PubMed1979

The effect of native and sonicated double-stranded polyribonucleotides on the course of spontaneous autoimmune disease in NZB and NZB/Swiss F1 mice.

The administration of sonicated fractions of f2 phage polyribonucleotides caused an increased weight loss and deterioration of the clinical state in female NZB mice. Discontinuance of the treatment resulted in an improvement of both the clinical state and the genetically determined autoimmune disorders of these mice. Some potential explanations of this effect are discussed.

Animals↗
PubMed1978

Results of a five-year study of the curative effect of double stranded ribonucleic acid in viral dermatoses and eye diseases.

Double stranded RNA obtained from non-permissive E. coli cells infected with f2-phage was tested in 5 hospitals in viral dermtoses such as herpes simplex recidivans, herpes zoster, male genikeratonconjunctivitis herpetica and conjunctivitis lignosa. The results of clinical tests indicate that the preparation of phage double stranded RNA applied topically is harmless for man and has, in the majority of cases, a beneficial effect in the disease. This conclusion was based on the judgment of physicians, opinion of patients expressed in a questionnaire, and, results of a double-blind experiment.

Clinical Trials as Topic↗
PubMed1978

[Effect of sodium chloride on the secretory activity on the kidney juxtaglomerular cells and on the adrenal glomerular zone in the adult male rabbit].

Experiments were carried out on the adult male rabbits. The animals were divided in three experimental groups and one control group. The animals of all experimental groups were given NaCl, through different ways. The degree of granularity of juxtaglomerular cells was evaluated by counting the JGI (juxtaglomerular index) according to HARTROFT. In all experimental groups, the JGI was lowered. The lowest was found in the first experimental group, that was given NaCl drinking fluid. The width of the zona glomerulosa was measured microscopically on the adrenal gland tissue colored by HE. It was smaller in all experimental groups. The smallest was in the first experimental group of animals. The diminution of the zona glomerulosa corresponds with the decrease of granularity of juxtaglomerular cells.

Adrenal Cortex↗
PubMed1977

Effect of impulsin treatment of interferon production and antiviral resistance of mice.

The effect of N-(2-hydroxyethyl)-palmitamide (Impulsin, Spofa)--an active endogenous compound which is recommended for preventing virus infection of the respiratory tract--on interferon production in mice stimulated with double stranded RNA and on the course of disease caused by encephalomyocarditis virus (EMC) was studied. Impulsin itself did not stimulate interferon production in mice treated per os or intravenously. But repeated application of this drug per os induced a macrophage activation, reflected by enhanced interferon production in vitro. When the interferon stimulation was delayed until 4 to 10 days after the first dose of Impulsin, interferon response to ds-RNA was slightly increased. After this phase of enhanced activity a decreased production of interferon was observed. Impulsin was not significantly effective in protecting mice from lethal dose of EMC virus. Application of this drug had an inhibitory effect on the toxicity of ds-RNA. A possible explanation of the mechanism by which Impulsin decreased the toxicity of virus in the organism is discussed.

Animals↗
PubMed1976

Persistent infection of BHK cells with herpes simplex virus types 1 and 2 in the absence of specific anti-herpetic antibody.

Persistent infection of BHK-21 cells with 5 strains of herpes simplex virus (HSV) type 1 and a 1 strain of HSV type 2 is described. Acute infection only was obtained with two strains of type 1 and one strain of type 2. Persistent infection could not be established in HeLa and rabbit lung cells. Persistent infection of BHK cells proceeded in the absence of specific antibody. Persistently infected cells were more resistant to superinfection with homologous or heterologous HSV as compared to infection of normal BHK cells. No interferon activity was demonstrated in persistently infected cell cultures. The results of virus titration were in accordance with the demonstration of fluorescent antigen of HSV.

Animals↗
PubMed1976

Increased resistance of an interferon-resistant cell subline to the toxic effect of double-stranded RNA.

The toxicity of double-stranded (ds) f2-phage RNA for interferon-treated L cells and an interferon-resistant subline of L cells (CVS cells) was compared. The interferon-resistant subline proved to be about 20 times more resistant to ds RNA than the parent L cells. The degree of toxicity of ds RNA was dependent on the concentration of interferon to which the cells were exposed.

Animals↗