Biomedical subjects
V Lees
Publications and source records attributed to V Lees.
Management of the chronic perineal sinus: not a problem to sit on.
It is our belief that the majority of cases of perineal sinus are curable if certain principles of wound management are followed. The causes and management of perineal sinus are reviewed. Four cases which illustrate the difficulties of achieving healing are reported, and from these important aspects of treatment are highlighted.
The methane monooxygenase gene cluster of Methylococcus capsulatus (Bath).
Methane is oxidised to methanol in methanotrophic bacteria by the enzyme methane monooxygenase (MMO). Methylococcus capsulatus (Bath) produces a soluble MMO which oxidises a range of aliphatic and aromatic compounds with potential for commercial exploitation. This multicomponent enzyme has been extensively characterised and biochemical data have been used to identify a 12-kb fragment of Methylococcus DNA carrying the structural genes mmoY and mmoZ, coding for the beta- and gamma-subunits of MMO component A, the methane-binding protein. We now report the complete nucleotide (nt) sequence of mmoX, the gene encoding the alpha-subunit of component A which is found to be 5' to mmoY and mmoZ. We also report the complete nt sequence of mmoC which encodes component C, the iron-sulfur flavoprotein of MMO, the N terminus of which is significantly homologous with spinach ferredoxin. The mmo structural genes are clustered within a 7-kb region and are closely linked to two small open reading frames of unknown function.
Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath).
Methane monooxygenase (MMO) is the enzyme responsible for the conversion of methane to methanol in methanotrophic bacteria. In addition, this enzyme complex oxidizes a wide range of aliphatic and aromatic compounds in a number of potentially useful biotransformations. In this study, we have used biochemical data obtained from purification and characterization of the soluble MMO from Methylococcus capsulatus (Bath), to identify structural genes encoding this enzyme by oligonucleotide probing. The genes encoding the beta and gamma subunits of MMO were found to be chromosomally located and were linked in this organism. We report here on the analysis of a recombinant plasmid containing 12 kilobases of Methylococcus DNA and provide the first evidence for the localization and linkage of genes encoding the methane monooxygenase enzyme complex. DNA sequence analysis suggests that the primary structures of the beta and gamma subunits of MMO are completely novel and the complete sequence of these genes is presented.
Zimbabwe three years on.
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A comparison of the use of polythene sheet and Jelonet as temporary dressings for excised wounds.
This paper reports our experience of the use of polythene sheet as a temporary dressing for excised wounds. A prospective randomised double-blind trial was conducted to see whether or not polythene dressing was less painful to remove than our traditional dressing of Jelonet. Polythene was found to be less painful (p less than 0.01). Other advantages of the use of polythene are presented.
Use of a pedicled fascial flap based on septocutaneous perforators of the posterior tibial artery for repair of distal lower limb defects.
A distally based fascial flap raised on perforating vessels of the posterior tibial artery is described. Its successful application in the repair of distal soft tissue defects of the lower limb in two patients is reported.