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V M Berzin'

Publications and source records attributed to V M Berzin'.

12 recordsLinked to original sources
PubMed1995

[15-19-mer synthetic oligoribonucleotide as a model of the translation initiation segment of the phage fr replicase gene].

Automatic solid-phase synthesis of 15-19-meric oligoribonucleotides was carried out using 5'-O-dimethoxytrityl-2'-O-(2-tetrahydropyranyl)- [or 2'-O-(2-tetrahydrofuranyl)-] N-acylribonucleoside-3'-O-(methyl-N, N-diisopropyl)phosphoramidite synthons and 1-H-tetrazole as an activator. Comparative analysis of the template activity of the oligoribonucleotides synthesized, which are models of the translation initiation region of the replicase gene of MS2 and fr phages, showed that the minimal active fragment of RNA is a 16-mer containing the initiation AUG codon of the gene, a short spacer, a Shine-Dalgarno domain, and the 5'-terminal AUGA sequence with a functionally important termination AUG codon.

Bacteriophages↗
PubMed1986

[Primary structure of a fragment of cDNA from phage fr].

The nucleotide sequence of a 1392 bp fragment of phage fr cDNA has been determined. The fragment contains 3'-terminal part of the A-protein gene, the complete coat protein gene, and beginning of the replicase gene. A comparison between the sequences of the corresponding genes and regulatory regions from the phage fr and MS2 genomes reveals 320 base changes.

Bacteriophages↗
PubMed1981

[Preparation of RNAase-free E. coli ribosomes active in initiation of protein biosynthesis].

The level of contaminating RNAases in the main components of the protein biosynthesis initiation system, the initiation factors and ribosomes of E. coli, was studied. It was shown that the ribosomes are the major source of contaminating RNAases. A simple procedure for purification of ribosomes active in initiation including Sephadex G-200 gel-filtration of unwashed ribosomes in a 1.5 M NH4Cl-containing buffer was developed.

Escherichia coli↗
PubMedDate not supplied

[Regulatory region of the MS2 phage RNA replicase cistron. Accesibility of MS2 RNA specific fragment to T1 RNAse digestion and chemical modification with kethoxal].

Partial digestion with T1 RNAase and chemical modification with kethoxal were used to study stability of two hairpin in the proposed secondary structure of the functionally active MS2 RNA fragment MS2 R(--53 leads to 6), containing the regulatory region of the phage replicase cistron. Analysis of the products obtained after the above treatments showed that T1 RNAase and kethoxal attacked predominantly the guanosine residues in the hairpin b of the MS2 R(--53 leads to 6). This implies that in contrast to the structurally stable hairpin a of the polynucleotide, hairpin b appears to be more labile and may exist under the present experimental conditions in equilibrium with its open form. The data of the competition experiments demonstrated that the kethoxal modified MS2 R(-53 leads to 6) and shorter polynucleotide MS2 R(-53 leads to-11) obtained from MS2 R(-53 leads to 6) after T1 RNAase digestion failed to bind with MS2 coat protein. The relatively unstable hairpin b region in the polynucleotide MS2 R(-53 leads to 6) is suggested to play essential role in the complex formation.

Aldehydes↗
PubMedDate not supplied

[Study of the mRNA-binding region of ribosomes at different steps of translation. II. Affinity modification of Escherichia coli ribosomes by benzylidene derivative of AUGU6 in the 70S initiation complex].

2',3'-O-(4-[N-(2-chloroethyl)-N-methylamino]) benzylidene derivative of AUGU6 was used for identification of the proteins in the region of the mRNA-binding centre of E. coli ribosomes. This derivative alkylated ribosomes (preferentially 30S ribosomal) with high efficiency within the 70S initiation complex. In both 30S and 50S ribosomal subunits proteins and rRNA were modified. Specificity of the alkylation of ribosomal proteins and rRNA with the reagent was proved by the inhibitory action of AUGU6. Using the method of two-dimensional electrophoresis in polyacrylamide gel the proteins S4, S12, S13, S14, S15, S18, S19 and S20/L26 which are labelled by the analog of mRNA were identified.

Alkylation↗
PubMedDate not supplied

[Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments].

A new set of short RNA templates has been prepared for functional studies in initiation of translation in vitro. Number of individual RNA fragments which contain complete or part of the initiatory region of phage fr replicase cistron were isolated from complex fr RNA--fr coat protein. Their primary structure were determined by using standard fingerprint technique and rapid gel sequencing. Secondary structure of several RNA fragments and their binding activity with phage fr and MS2 coat proteins has been also studied.

Amino Acid Sequence↗