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V M Mazhul'

Publications and source records attributed to V M Mazhul'.

29 records · Page 2Linked to original sources
PubMedDate not supplied

[Change of state of erythrocyte membrane proteins during storage of preserved blood].

Erythrocyte vesiculation, kinetic parameters of tryptophane phosphorescence at room temperature and activity of erythrocyte membrane during storage of human blood at 4 +/- 2 C were studied. It is shown that before the erythrocyte vesiculation stage (10-15 days of storage) two types of changes in the structural state of the membrane proteins occur: 1) on the 1-5th days and 2) on the 5-10th days of blood storage.

Acetylcholinesterase↗
PubMedDate not supplied

[Structuro-functional characteristics of aldolase from rabbit muscles in diabetes].

The structural peculiarities of rabbit muscle aldolase accompanying enhancement of the aldolase activity in diabetes are described from the data of tryptophan phosphorescence at the room temperature and fluorescence polarization. It is shown that the pathology-concomitant conformational changes occur in both the hydrophobic part and NAD-binding site of the enzyme. The character of the structural changes in the hydrophobic part of the protein in diabetes and an increase in the enzymic activity are similar to that observed in normal aldolase after its interaction with NADH and are believed to be associated with the enhancement of the rigidity in the Trp-147 environment.

Animals↗
PubMedDate not supplied

[Study of the role of microtubules in the regulation of activity of highly permeable contact membranes].

It was shown that various antitubulines-colchicin (10(-4) M), vinblastin-sulfate (10(-5) M) and vinoristin-sulfate (10(-5) M) do not affect the intercellular diffusion exchange of small inorganic ions and organic dye fluorescein in Drosophila virilis larva salivari gland. It is suggested that microtubules do not take part in functional activity regulation of gap junction high-permeable membranes.

Animals↗
PubMedDate not supplied

[Effect of falloidin on permeability of intercellular channels for inorganic ions and fluorescein].

It has been shown on larva gland that falloidin - a toxic of toadstool inducing an increased number of membrane microfilaments-affects the efficiency of intercellular transfer of small inorganic ions and molecules of organic dye fluorescein-Na. The data obtained are in favour of the participation of microfilaments in the regulation of intercellular diffusion communication.

Animals↗
PubMedDate not supplied

[Study of the equilibrium dynamics of cell protein structure using the tryptophan fluorescence method at room temperature].

Room-temperature tryptophane phosphorescence (RTTP) of liver tissue cells has been studied. It is shown that over a millisecond range RTTP is absent in soluble proteins of the cytoplasm, karyoplasm, mitochondrial matrix, and the phosphorescent signal is controlled only by proteins of the subcellular structures incorporated into the membranes. It is concluded that, unlike the membrane proteins, the cytoplasm and organelle matrix-soluble proteins are characterized by a high level of intramolecular equilibrium mobility, which causes RTTP quenching following a dynamic mechanism. In membrane proteins, which fluoresce in a millisecond range the level of equilibrium conformation motions is limited, probably, due to protein-protein and protein-lipid interactions.

Animals↗
PubMedDate not supplied

[Low temperature phosphorescence of lipid peroxidation products].

The phosphorescence exitation and emission spectra and the phosphorescence lifetimes of polymerized malonic aldehyde, Shiff bases, linoleic acid, phosphatidylcholine, phosphatidylethanolamine, cardiolipine, total lipid fraction from human erythrocyte membranes were measured at 77 K. The nature of chromophores of lipid peroxidation products capable of phosphorescence was discussed.

Animals↗