PubMed Health⌕ Search

Biomedical subjects

V M Mikhaĭlova

Publications and source records attributed to V M Mikhaĭlova.

6 recordsLinked to original sources

[Identification and characterization of strains of Bacillus thuringiensis by genomic fingerprinting using biotinylated phage M13 DNA].

Genomic DNA of the entomopathogenic bacterium Bacillus thuringiensis was analyzed by the genomic fingerprinting technique. The biotin-labeled single-stranded DNA of the phage M13 was used as a marker of hypervariable sequences. A procedure for analyzing the differentiation among various Bacillus thuringiensis strains was developed. Characteristic patterns of fingerprints were obtained for several strains, the main representatives of subspecies that are most frequently used in the manufacture of bacterial insecticides, such as subsp. thuringiensis, subsp. kurstaki, and subsp. galleriae. Because no essential differences were revealed in band patterns upon comparing fingerprints of crystal-producing bacterial strains with those of acrystallic mutants, it was assumed that the loss of crystal-producing ability in the insect pathogen Bacillus thuringiensis is not connected with significant rearrangement of its genome.

Bacillus thuringiensis↗

[Effect of diet and drug therapy on the rehabilitation processes in patients who have undergone radical surgery for stomach tumor].

On the basis of the follow up of 141 patients after radical operations for gastric cancer, special diet variants have been developed with due regard for postoperative terms and the character of complications after gastric resection. Positive results of dietotherapy were recorded in 71.5% of the patients, that was evidenced by their clinical picture and laboratory data: coprological, biochemical and immunological. The condition was not changed in 15.6% of the patients, negative shifts were noted in 18 patients (12.7%), in 12 of them relapses or metastases of the tumor were recorded.

Adult↗

[An increase in the effectiveness of outpatient care of patients with kidney diseases].

Organization of medical care of patients with renal diseases in a municipal health center is described. The authors demonstrate the efficacy of specialized care when it was made available for population. The detection of renal diseases improved. Use of modern methods of diagnosis and treatment, day-time hospitals, and regular check-ups permit timely comprehensive qualified care of patients with renal diseases, prevention of disease progress and chronic development, prolong the capable life of young patients, and decrease the incidence of disability.

Adult↗