Biomedical subjects

V M Panin

Publications and source records attributed to V M Panin.

21 records · Page 2Linked to original sources

PubMedDate not supplied

[Dependence of the chromatin condensation process on the temperature and ionic strength of the media: investigation using gel electrophoresis in low density agarose].

Conformational mobility of 30 nm nucleosomal fiber under temperature variations at different ionic strength of the medium has been analyzed. A new property of chromatin organization has been characterized, namely, nucleosomal solenoid condensation in response to increase of temperature. This property is analogous to salt-induced condensation and becomes particularly prominent at low ionic strength. In this context previously obtained data on changes in the DNA linking number of topological domains are discussed. It is concluded that these changes probably arise from modification of supernucleosomal level of chromatin organization.

Animals↗

PubMedDate not supplied

[Study of the process of chromatin decondensation using low density agar gel electrophoresis].

A method was proposed for analysis of conformational mobility of supranucleosomal chromatin organization at different ionic conditions with the help of electrophoresis in low-density agarose gels. This simple and highly reproducible method yields the results which are in good agreement with the data of other traditional approaches. This method offers an alternative to high-speed ultracentrifugation for chromatin condensation studies.

Animals↗

PubMedDate not supplied

[Interconnection of hierarchical compactization levels as the basis for functional organization of chromatin].

Recent data on chromatin structural organization are presented. In the light of present concept of chromatin structure the problem of hierarchical levels of DNA packaging within chromatin is discussed. Particular emphasis has been placed on the correlation between the changes occurring at different DNA compactization levels within topological domains and chromatin functional activity.

Chromatin↗