Biomedical subjects

V M Starodub

Publications and source records attributed to V M Starodub.

9 recordsLinked to original sources

PubMedDate not supplied

[Immune sensor for determining myoglobin level].

For the first time, the photoluminescence of porous silicon was used to create an immune sensor capable of detection of low concentrations of myoglobin important for the diagnostics of heart failure diseases which in both model solutions and human serum, being. It is shown that the sensitivity of the sensor was 10 ng/ml, time of 1 measurement--15-30 min. Duration of the analysis can be even shorter if measurements are performed in the kinetic regime. The comparison of the characteristics of the immune sensor and standard method ELISA is also given in the paper.

Biosensing Techniques↗

PubMedDate not supplied

[A new type of electrochemical immunosensor based on ion-selective field effect transistors].

A new type of an electrochemical sensor based on the use of ion-selective field effect transistors (ISFETs) and conjugates of horse radish peroxidase with specific monoclonal antibodies was developed for the express determination of myoglobine in a solution. For this purpose a simple method of covalent immobilization of myoglobine on the surface of ISFET gate was worked out, an appropriate biochemical approach which allowed potentiometrical registration of the peroxidase activity was used, and an immune chemical analysis was accomplished in competitive way. It was shown that the sensitivity of the analysis with the help of the electrochemical immune sensor corresponds to the demands of medical practice to reveal early stages of myocardial infarction. This sensitivity was significantly higher then that which can be obtained by the traditional ELISA-method. Moreover, overall time of the analysis by the immune sensor was almost one order shorter than this by the ELISA-method. It is concluded that the proposed electrochemical immune sensor based on the ISFETs was very perspective for the express analysis of the level of different types of antigens and antibodies.

Antibodies, Monoclonal↗

PubMedDate not supplied

[Immunosensors: sources of origin, achievements and perspectives].

The analysis of the recent data in the literature and results of investigations in the field of the development and study of function efficiency of different types of immune sensors, that are performed at the Department of Biochemistry Sensory and Regulatory Systems of the A. V. Palladin Institute of Biochemistry of Ukrainian National Academy of Sciences are presented. Sources of origination and perspectives of the devlopment of biosensors are discussed as well. The paper also gives an overview of main research projects at the Department, mainly in the filed of biosensors. They include development of the scientific bases for the creation of a new generation of chemo- and biosensors for their application in medicine and ecology. Multi-immune, multi-enzyme and combined multi-parametrical sensors can provide express analyses in laboratory and field conditions with the purpose to perform immune chemical diagnostics of diabet, kidney diseases, immune defficiencies, autoimmune, allergic, pre-infarction and pre-tumor states as well as to control total toxicity of the environment and identification of main types of toxic elements in it. The investigations are based on the latest achivements in the field of physics, chemistry, information technology and electronics with the use of different types of planar electrodes, ion sensitive field effect transistors (ISFETs), semiconductor capacitive structures, termistors, optrodes, piezocrystalls and application of such methods and effects as laser correlation spectroscopy, chemiluminescence, fluorescence, surface plasmon resonance, photoluminescence of porous silicon, interferometry, evanescent wave technique, nonemmiting energy transfer and holography.

Allergy and Immunology↗

PubMedDate not supplied

[Enzymatic sensor for determing triglycerides].

An enzymatic sensor based on ion selective field effect transistors was developed for the determination of triacylglyceroles in the range of concentrations from 0.1 to 2 mM. It was show that the sensitivity of the sensor was equal to 0.1 mM and time of one measurement was 2 min. The optimal environment for the triacylglyceroles registration is 1 mM sodium phosphate buffer (pH 7.3).

Biosensing Techniques↗

PubMedDate not supplied

[Specific interaction of biological molecules. A new method of registration].

The experimental results on influence of some biological molecules and their specific immune complexes on the current-voltage characteristic of surface-barrier contact structures (Ni-Si or PorSiSi) are presented. It was shown that the deposition of the myoglobin and specific to its monoclonal antibodies or its specific immune complex caused the modification of the current-voltage characteristic. Moreover, the structures more essentially react to the formed specific immune complex deposition. The optimum thickness of the metal film or porous silicon layer necessary to the development of maximum response have been obtained. It was concluded that this electrometrical method was suitable for express determination of the formed specific immune complex without separation of it from the initial components of reaction.

Antibodies, Monoclonal↗

PubMedDate not supplied

Immobilization of biocomponents for immune optical sensors.

Immobilisation of both human immunoglobulin(IgG) and antiimmunoglobulin (anti-IgG) was performed by means of polyelectrolyte self-assembly. This technique was compared with direct immobilisation of the immune components on bare gold and their covalent binding via glutaraldehyde as a bifunctional reagent. Additionally, the immune components were properly oriented during their immobilisation by using a predeposited layer of the protein A. Methods of the surface plasmon resonance (SPR) and planar interferometry were employed for monitoring the immobilisation as well as specific immune reaction. It was shown that in case of the use of polyelectrolyte self-assembly it is possible to achieve the sensitivity of the analysis up to 30 ng/ml for SPR and up to 1 ng/ml for planar interferometer based immune sensors.

Autoantibodies↗

PubMedDate not supplied

[Analysis of specific nucleotide sequences. DNA biosensors].

Information about common molecular-biological approaches for the determination of the specific nucleotide sequences in genetic materials was given in the review. Main attention was paid to consideration of the ways for DNA biosensor creation. The information about the types of such biosensors was presented in detail and characteristics of the developed devices were cited. Separately the question about the use of the instrumental analytical approaches for the identification of genetic materials of individual pathogenic microorganisms was viewed.

Animals↗

PubMedDate not supplied

[Directed immobilization of recognizing elements of biosensor transducers].

Data about the directed immobilisation of recognising structures on metal and silicon surfaces of biosensor transducers are analyzed in the review. Attention is paid to such approaches as pretreatment of surface by silanes, thiol compounds as well as to formation of polyelectrolyte-based layers.

Animals↗

PubMedDate not supplied

[Study of sorption properties of organosilanes to be used as the basis for hemosorbents and diagnostic enzyme and immunosensors].

The abilities of different types of organosilanes, in particular of polymers with: 1) completely or 2) partially hydrophobical surfaces; 3) regular changes of part of silicium ions by metal ions; 4) preliminary aminosilanization were studied for sorption of ammonia ions, urea, cholesterol, creatinine, albumin, IgG, haemoglobin and myoglobin. Polymethylsiloxane was used as haemosorbent for directed sorption of myoglobin and haemoglobin from solution and blood. It didn't hemolysate red cells. The high efficiency of those organosilanes for sorption of haemoproteins it was shown. Organosilanes were very good as membrane for immobilization of urease and IgG-specific antibodies to create enzyme sensor and immunosensor based on the ionsensitive field effect transistors. The advantages and possibilities of organosilane usage as haemosorbents in the field of medicine of catastrophes as well as for sensor technology are discussed.

Adsorption↗