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V Matousek

Publications and source records attributed to V Matousek.

17 recordsLinked to original sources
PubMed1979

Xenogeneic bone marrow stimulating effect in vitro on antibody-producing cells.

It was described earlier that in the tissue cultures of immune mouse LN cells the number of antibody producing cells was increased 2-3-fold when syngeneic of allogeneic nonimmune BM cells were included in the cultures. In these experiments, parallel mixed cultures were set up of mouse immune LN cells with non-immune BM cells of either syngeneic or xenogeneic origin (rat, pig and chicken). Xenogeneic BM also increased the number of PFC in the mixed cultures. The intensity of the stimulation effected by xenogeneic BM of all three species tested was comparable to that induced by syngeneic BM. The number of PFC in all types of the mixed cultures was significantly higher (P less than 0.005) than in the LN cell cultures alone. The differences between the effect of syngeneic and xenogeneic BM and between the xenogeneic BM cells of different origin were not statistically significant (P less than 0.05).

Animals↗
PubMed1979

Dissociation of the proliferative and effector non-H-2 minor histocompatibility-alloimmune responses.

The first-set and second-set allotransplantation reactions against skin grafts and the primary and secondary proliferative graft-versus-host reactions in the popliteal lymph nodes were compared in both directions in a non-H-2 system (mouse strain combinations C57BL/10ScSnPh (further B10) and B10.C3H(40NX) further 40NX) differing at H-1 plus H-?). While 40NX recipients gave stronger reactions against B10 antigens in the allotransplantation reactions, the situation was reversed in the GVHR, B10 cells reacting more strongly against 40NX antigens. The findings of a dissociation between the mechanisms of allotransplantation reaction adn proliferative GVHR suggest that the genetic determination of the target antigens and the reacting lymphocyte populations are more complex at the minor histocompatibility systems than has been expected.

Animals↗
PubMed1978

Intercellular adhesiveness of H-2 identical and H-2 disparate cells.

Using a 2 X 2 design, the collection rates of cells from radioactively labelled single-cell suspensions on cell-coated collecting surfaces were tested for a possible H-2 effect on intracellular adhesiveness. By a statistical procedure, the undesirable variability in the suspension and/or in the collecting surface could be eliminated, and the H-2 specific net effect estimated. In this way it was possible to detect (even on the background of a large non-specific variability) significant reductions of the allogeneic collection rates (i.e. between cells which differed only in their H-2 haplotypes) as compared to the syngeneic standards. It is concluded that cells of a given H-2 haplotype have preferential adhesiveness to syngeneic cells.

Animals↗
PubMed1978

[Biochemical and hematological changes in the blood of horses after the "Velká Pardubická" steeple chase].

Blood parameters were studied in two groups of horses in the "Velká Pardubická" steeple-chase in 1974, 1975 and 1976. After the race, the levels of lactate showed a manifold increase; an increase was also ascertained in the levels of glucose, sodium, potassium, haemoglobin, in the haematocrit value and in the number of erythrocytes. The following parameters significantly dropped: the levels of acid-base balance - pH, base excess, bicarbonate levels. It was proved that the values of the same parameters in horses during training were incomparably lower. It is advisable to examine horses thoroughly during training and to use the results of training for the evaluation of their condition before difficult races.

Animals↗
PubMed1977

Cellular cooperation at the level of antibody-producing cells in chickens.

The effect of in vitro cocultivation of immune and non-immune syngeneic lymphoid cells on the antibody response was studied in chickens. Cocultivation of immune and non-immune spleen cells did not affect significantly the PFC numbers. Substantial increase in PFC was observed in mixed cultures of immune spleen cells with non-immune bone marrow cells. The existence of an enhancing effect of non-immune cells on the antibody-producing cells, which had been earlier described in mice, was observed in a phylogenetically distant species. Therefore it seems probable that it exists in most, if not all, homoiotherm animal species.

Animals↗
PubMed1977

Time dependence of the effect of splenectomy on graft-versus-host reactivity of lymph node cells.

Changes in cell-mediated reactivity of lymph node cells at various intervals after splenectomy were investigated in three assays measuring the GVH reactivity of parental cells in F1 hybrids --splenomegaly test in very young recipients and popliteal lymph node enlargement assay in adults measuring the proliferative component of the reaction, and mortality assay in sublethally irradiated recipients measuring the killer activity of the cell inoculum. During the early postsplenectomy period the reactivity of the particular amounts of lymph node cells was lower than that of cells from normal donors, but at about 3 weeks after splenectomy it was higher. The increase was of short duration in the proliferation assay and at 5 weeks the reactivity declined markedly below the control values. The increase in activity persisted for 5 weeks after splenectomy in the "killer" assay. It is probable that the described changes in cell-mediated reactivity are involved in the total effect of splenectomy on the host's complex immune response, especially against normal and tumour allografts.

Animals↗
PubMed1976

Mutagenic effect of epichlorohydrin. I. Testing on human lymphocytes in vitro in comparison with TEPA.

The mutagenic effect of the monofunctional alkylating agent epichlorohydrin was tested on human lymphocytes in vitro and compared with the mutagenic effect of the polyfunctional alkylating agent TEPA. The same descending concentrations were used for both mutagens: 10(-4), 10(-5), 10(-6), 10(-7), 10(-8), 10(-9), 10(-10) and 10(-11) M. Similar types of chromosomal aberration were found, but the effect of ECHH was 4-5 times lower than that of TEPA. ECHH was found to be a mild mutagen. Different timing of mutagen application was used in the course of 56 h of cultivation of lymphocytes: 1 h before cultivation, one hour between the 24th and 25th h of cultivation and 24 h before the end of cultivation. From the results presented we conclude that the application of the chemical for the last 24 h of human lymphocyte cultivation should be recommended for routine mutagenicity testing.

Azirines↗
PubMed1976

Attempt at "immunological castration" as an approach to the problem of the involvement of testosterone in control of expression of certain mouse antigens.

Adult male mice of the strain B10.A were immunized with a testosterone-protein conjugate, testosterone 3-(O-CARBOXYMETHYL)-oxime-bovine serum albumin which contained 27-75 steroid residues/mol BSA. Two different immunization doses of the conjugate were used, respectively, 2 x 40 mug and 2 x 200 mug in complete Freund's adjuvant or in alum adjuvant. There were two groups of control males, non-immunized and immunized with BSA in adjuvant. In the pooled immune sera, antibodies to testosterone were determined by radioimmunoassay; their titre ranged between 7 and 10. On histological sections of testes, inhibition of spermatogenesis (manifested by a sower frequency or even absence of tubules producing mature sperm, reduced frequency of tubular cells and their degenerative changes) was observed in almost all males immunized with the higher dose of the conjugate. In such animals, increased frequency of interstitial cells (except vascular elements) and enlarged nuclei of Leydig cells were found. In spite of these signs of a hyperproduction of testosterone by the Leydig cells, the product seemed to have lacked its normal biological activity as suggested not only by the low activity of spermatogenesis, but also by a significantly subnormal level of the androgen-dependent serum protein Ss.

Animals↗
PubMed1976

Quantitative characteristics of antibody forming cells in partial tolerance.

Direct PFC to SRBC were enumerated simultaneously by the LHL and LHG in the spleens of partially tolerant and control rats. The sensitivity of LHL was higher than that of LHG, but the factor by which the number of PFC was higher in LHL than in LHG was in the two groups similar (1.44 in tolerant and 1.47 in control group). When the plaque size distribution was studied in the LHL, a significant increase in small size plaques was observed in tolerant rats. The findings are discussed and the conclusion is reached that the decrease in AFC number and not in antibody production by individual PFC is the main factor responsible for partial tolerance.

Animals↗
PubMed1975

Attempts to compare the effectiveness of blocking factors and enhancing antibodies in vivo and in vitro.

Sera from rats carrying tolerated skin allografts were tested for the presence of blocking activity in vitro. Sera with blocking activity had no effect on transplantation tolerance induction in newborn animals. Immunological enhancement of tumor growth was procured by passive transfer of serum from tolerant animals bearing skin allografts. It made no difference whether or not the serum contained blocking activity in vitro. These results suggest that there is no relationship between blocking factors and enhancing activity in vivo.

Animals↗
PubMed1975

H-2-associated quantitative traits; statistical analysis of the relative contributions of the H-2 linked genes and the residual genotype.

Several associations have been recently reported of the kind that a certain H-2 haplotype tends to be accompanied by a low value and another H-2 haplotype by a high value of some quantitative traits. This includes also the weights of lymphoid and reproductive organs. In order to throw more light on the seeming clustering within the H-2 gene complex of the genetic determinants of such traits, statistical analysis of the relative contributions of the H-2 and the total residual genotypes to the variations in the weight of the thymus, spleen, lymph nodes, testes, seminal vesicles and ovaries was performed. Ten genotypically uniform populations of 2-month-old mice, each consisting of 31 males and 31 females, were analyzed using two different statistical procedures. Four of them were mice of the inbred strains A (H-2a), B10 (H-2b), A.BY (H-2b) and B10.A (H-2a) and six were various F1 hybrids derived from these four strains. The conclusion based on both approaches was that the relative contribution to the variations in the weight of the tested organs from the H-2 haplotype is much smaller or maximally not greater than the contribution from the total residual genotype.

Animals↗
PubMed1975

The relative effect of H-2 disparity between irradiated host and injected bone marrow cells on their total proliferative and erythropoietic activities in the host's spleen.

Previous experiments showed that in the spleen of non-syngeneic (H-2 disparate) irradiated hosts, the colony-forming performance of injected bone marrow cells may be markedly lower than the standard syngeneic performance. There were also some indications that the deficit was due to a deviated differentiation of the pluripotent stem cells into an alternative pathway. In the present study, the total proliferative activity of donor bone marrow cells [B10.A(2R)] in the syngeneic or allogeneic [B10.A(5R)] hosts was measured as the rate of uptake of 125IUdR and the erythropoietic activity as the rate of uptake of 59Fe. Since both activities are linear functions of the number of injected cells, their inhibition in allogeneic hosts could be judged by the relative slopes of the comparable regression lines. Well reproducible results showed that the erythropoietic activity was inhibited more than the total proliferative activity; this suggests that the residual component of the total proliferative activity must be inhibited less (or even stimulated) in the allogeneic host. The possibility that the differential effect of the allogeneic host environment on the total and erythropoietic activities might be due to a deviated differentiation of some pluripotent cells into the lymphopoietic pathway is discussed.

Animals↗