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Biomedical subjects

V N Grigor'ev

Publications and source records attributed to V N Grigor'ev.

16 recordsLinked to original sources
PubMed1992

[Possibilities of restoration of the regional lymphatic channel by the use of sorbents in the treatment of experimental generalized purulent peritonitis].

Regional lymphatic bed in local application of mineral sorbent SUMS-2p in 80 white noninbred rats with experimental generalized purulent peritonitis is studied with the use of indirect intravital lympho-roentgenography. The correlation between lympho-dynamics and generalized purulent peritonitis stage was observed. It is shown that the peritoneo-sorption with the mineral sorbent SUMS-2p led to early restoration of lympho-drainage.

Animals↗
PubMed1991

[Structural changes in the lymph node sinuses in acute blood loss].

By means of light and scanning electron microscopy sinuses of the popliteal lymph nodes have been studied in 50 Wistar male rats. After modelling of an acute hemorrhage in the animals (in 15, 120 min and 1 day) volume of the medullary and marginal sinuses increases essentially, while blood stream intensity decreases in peripheral tissues. Amount of fenestrae in the sinusal lining decreases, that is, perhaps, caused by edema of cytoplasm in the lining cells and accompanied with formation of numerous cytoplasmic protrusions into the sinusal lumen. In 3 days the blood stream level in the peripheral tissues restores, structural organization of the littoral cells in the lymphatic sinuses normalizes. The data obtained demonstrate that during the first day after the acute hemorrhage decrease of hematolymphatic exchange intensity in lymph nodes and enhancement of transitory lymph flow in them take place.

Acute Disease↗
PubMed1990

[Comparative study of the changes in microanatomical organization of lymph nodes located and functioning in the area of venous congestion].

By the morphometric method the microanatomical organization of the popliteal and kidney lymph nodes of the type Vi was studied in venous congestion. In the first experimental series (lymph nodes localize in venous congestion area) the venous congestion in the popliteal lymph nodes was created by the ligature of caudal vena cava. In the second experimental series (lymph nodes, which clean the venous congestion area--the kidney vena was tied up. The small and short time increase of the marginal sinus volume and hypertrophy of the medullary cords, which seemed about medullary substance B-zone stimulation were characteristically for the lymph nodes, localize in the venous congestion area. The sharp increase of the marginal sinus volume during the long experimental time and paracortical T-zone hyperplasia were characteristically for the lymph nodes, which clean the venous congestion area.

Animals↗
PubMed1989

[Microanatomical organization and cytoarchitectonics of lymphoid tissue in the mesenteric lymph nodes under conditions of experimental total hyperthermia].

Microanatomical organization and cell composition of the rat mesenteric lymph nodes have been studied under conditions of experimental total hyperthermia. Possibilities of the compensatory-adaptive reactions of the lymphoid tissue have been determined. Disturbances of microhemo-++- and lymphocirculation in the lymph nodes in different time after hyperthermia are accompanied with accumulation of mast cells and eosinophils in various zones of the node; they normalize certain physiological parameters of microcirculation and permeability of the metabolic microvessel walls. In 30 days after a single total overheating of the rat organism, the microanatomical organization changes in the lymph nodes investigated are manifested as atrophy of B-dependent zones and further volumetric increase of the paracortical zone. Therefore, it is possible to think about a decreasing intensity of the humoral immunity and increasing intensity of the cellular one.

Animals↗
PubMed1988

[Structural organization of post-capillary venules in the somatic lymph nodes during total overheating of the body].

The structure of the postcapillary venules in the rat lymph nodes has been studied after a single total overheating at 43.5 degrees C. During the first three days an essential role is played by ultrastructural destructive processes, that demonstrate certain unfavourable states in cells. Nevertheless, the postcapillary venules perform their function quite satisfactory and even increase small lymphocytes transfer. The 7th-15th days are characterized with a steady compensation of the functional activity of these microvessels. By the 30th day some unfavourable changes in the endothelial ultrastructure are noted. This is, evidently, connected with a steady effect of the plasma toxic factor on the endothelium.

Animals↗
PubMed1988

[Ultrastructural organization of the endotheliocytes of iliac lymph node capillaries in pregnancy (morphometric study)].

Using light and electron microscopy and morphometry, the morphological changes in the lymph nodes of arterial and venous parts of capillaries were studied on the 11th, 17th and 21st days of pregnancy in rats. Ultrastructural changes in endothelial cells of blood vessels in the uterine lymph nodes during normal pregnancy are of adaptive nature and are possibly responsible for the relief of the blood congestion in the system of the inferior vena cava and for the improvement of the utero-placental circulation.

Animals↗
PubMed1987

[Ultrastructural organization of capillary endotheliocytes of the popliteal and iliac lymph nodes in the rat].

The popliteal and iliac lymph nodes have different regional specialization. The former belong to somatic lymph nodes, while the latter get lymph also from internal organs; this explains certain differences in their structural-functional organization. Some objective quantitative data have been obtained that characterize ultrastructure of blood capillaries in the rat popliteal and iliac lymph nodes under normal conditions of the organism's functioning. They demonstrate essential differences in the level of transendothelial transport and, hence, functional activity of these organs.

Animals↗
PubMed1987

[Morphometric study of the ultrastructural organization of the endotheliocytes of blood capillaries in the iliac lymph nodes of pregnant rats].

The ultrastructure of blood capillary endotheliocytes of iliac lymph nodes during pregnancy was investigated in rats. It was found that changes in microcirculation during pregnancy are accompanied by the appearance of the adaptive changes at all levels of lymph node structural organization. They are: increased lumen of arterial and venous part of the capillaries, increased intermediate sinus volume, increased fenestration of both parts of capillaries, a sharp increase in the number of micropinocytous vesicles and activation of transendothelial channel formation.

Animals↗
PubMed1985

[Structural organization of the compensation process in the lymph nodes in venous stasis].

Transmission and scanning electron microscopy was used to study the structural organization of the lymph nodes during venous congestion. At the early period (1 h of venous congestion) one could see an increased number of the fenestrated cells in the venous part of the capillary whereas at the later period (after 6 h to 3 days) a rise of the microvesicular transport via endotheliocytes of the arterial part of the capillary and via fenestras of the venous part. Within 7 to 14 days one could see activation of the microvesicular transport via the indicated parts of the capillary. At all the stages of venous congestion the fluid contained by the parenchyma of the lymph node penetrates via fenestras and intercellular fissures to the marginal sinus, while only via fenestra to the cerebral sinuses.

Adaptation, Physiological↗
PubMed1985

[Littoral cells of the lymph node sinuses in venous congestion based on scanning electron microscopic data].

The littoral cells of the sinuses in the popliteal lymph nodes have been studied in Wistar rats under physiological conditions of hemodynamics and 1 h after ligation of the caudal vena cava. Intercellular clefts (1-4 mcm wide) are demonstrated to exist between the littoral cells of the internal wall of the marginal sinus. Presence of fenestrae in the peripheral thinned areas of cytoplasm is specific for these cells. The diameter of the fenestrae is 60-1,000 nm however, the fenestrae with the diameter up to 100 nm predominate. Lining of the medullar sinuses is also characterized by a high content of fenestrae, the maximal diameter of the fenestrae being the same as in the marginal sinus, although most of the fenestrae have the diameter 50-500 nm. No open intercellular clefts between the littoral cells of the medullar sinuses are revealed. In some cases, penetration of lymphocytes through the lining of the sinuses is observed. Under venous congestion, the diameter and amount of the fenestrae, size and amount of open intercellular clefts in the lining of the lymphatic sinuses increase significantly. This demonstrates its increasing permiability, having an essential importance for compensation of circulatory disturbances in the zone of the venous congestion.

Animals↗
PubMed1983

[Ultrastructure of the endothelial cells of blood capillaries of lymph nodes in the rat].

Ultrastructure of endothelium in the rat popliteal lymph nodes has been studied. The cross section area of the capillary arterial part is 29.9 +/- 1.0, that of the venous part -- 91.2 +/- 1.6, the luminal cross section of the arterial part is 4.90 +/- 0,23, that of the venous part -- 46.8 +/- 1.1 mcm2. Nucleous-cytoplasmic ratio in the endotheliocytes of the arterial part is 1.50 +/- 0.06, that of the venous part -- 2.10 +/- 0.13. The number of microvesicles per 1 mcm of the profile length in the section of the basal surface in a plasmolemma is 1.13 +/- 0.07 both in the arterial and venous parts of the capillary, that of the luminal surface -- 1.35 +/- 0.07 for the arterial part and 0.48 +/- 0.03 for the venous part. The number of free microvessels per 1 mcm2 of the cytoplasmic cross section area is also greater for the arterial part (9.6 +/- 0.8 vs. 7.3 +/- 0.4 in the venous part). Numerical density of free ribosomes in endotheliocytes of the arterial part is 62 +/- 6, in the venous part -- 47 +/- 5 per 1 mcm2 of the cytoplasmic cross section. Numerical density of mitochondria in the venous part is 0.77 +/- 0.11 and in the arterial part -- 0.40 +/- 0.04 per 1 mcm3 of cytoplasm.

Animals↗
PubMedDate not supplied

[Rationale of noninvasive method of drug administration at the prelymphatic level].

The paper deals with the mode of local drug administration into the biological tissue at the prelympathic level without damaging the skin, which is based on enhancement of skin permeability by exposing to physical factors, including the use of contrast temperatures in the cyclic process of heating-cooling, followed by exposure to contact low-frequency ultrasound. Experimental and clinical studies of the mode versus routine drug administration have shown it to be promising in physiotherapeutic use.

Animals↗