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V N Iakovlev

Publications and source records attributed to V N Iakovlev.

59 records · Page 4Linked to original sources
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[Isolation of virus-specific double-stranded RNA from cells transformed with Rous sarcoma virus].

Several methods were used for isolation of double-stranded (ds) RNA from the cytoplasm of Rous sarcoma virus-transformed chick embryo cells. The dsRNA was shown to have a high melting temperature (82.5 degrees C) in 0.16 M phosphate buffer (pH 6.8), which shifted to more than 90 degrees C after RNase treatment. The size of a single strand was approximately 1300-1600 nucleotides and RNase-resistant fragments were 50-250 nucleotides long. Double-stranded RNA formed hybrids with the labeled genomic RSV RNA RNA so that the major subpopulation of the dsRNA hybridized to 6-10% of RSV RNA and the minor subpopulation -- to 90-94% of RSV RNA. It was suggested that this large subpopulation of dsRNA was abundant in sequences homologous to proviral end fragments as judged by Southern procedure. The data are discussed by considering the analogy between retroviral proviruses and mobile genetic elements.

Animals↗
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[Role of hyperbaric oxygenation in the mechanism of ammonium detoxication in resection of the liver in the presence of chronic hepatitis].

Hyperbaric oxygenation (HBO) used in 170 white rats with chronic CCL4-hepatitis after resection of the liver was conducive to repair of the reversible (glutamine synthesis) and irreversible (urea synthesis) routes of ammonium binding in hepatocytes which are disordered in chronic hepatitis. Hyperbaric oxygen regulates the effect of resection of the liver on the glutamine- and urea-synthesizing function of the hepatocytes in chronic hepatitis. Therapeutic effect of HBO persists for 4 days of the posthypoxic period under conditions of posthyperoxic hypoxia.

Animals↗