Biomedical subjects

V N Kharlamova

Publications and source records attributed to V N Kharlamova.

10 recordsLinked to original sources

PubMed1980

[Infrared spectra of certain proteoglycans].

IR spectra of high-purified potassium salts of hyaluronic acid (HA), non-aggregation fraction of protein-chondroitin-keratan sulfate (PChKS) and heparin fractions containing 3 (H-3) and 4 (H-4) residues of sulfuric acid per dimer of polymer have been measured. An absorption band of 1150 cm-1 has been observed in the spectra of both HA and PChKS but has not been found in the spectra of H-3 and H-4. Observation of this band in the spectra of some heparin preparations indicates that they contain the admixtures of HA, PChKS and other proteoglycans. A previously suggested method for isolation of heparin fractions H-3 and H-4 makes it possible to obtain preparations that do not contain the admixtures of other proteoglycans.

Animals↗

PubMed1979

[Electron microscopic study of isolated proteoglycans].

Chemically isolated separate preparations of the non-aggregating protein-chondroitin-keratin sulphate (PCKS) fraction from the hyaline cartilage and hyaluronic acid (HUA) of the vitreous body and of the umbilicus were investigated by electron microscopy. PCKS and HUA in films without cytochrome c were present in the form of granules and differed by structural organization. The proteoglycans of the cytochrome c films were seen as finely-filamentous-cellular network, and were distinctly differentiated by their macromolecular organization. A mixture of both proteoglycans formed complexes as a result of a noncovalent interaction. Uranyl acetate ensured a good contrasting of proteoglycans, especially of PCKS, without cytochrome c.

Acetates↗

PubMed1976

[Structural-mechanical properties and swelling of the protein complexes of hyaluronate and protein-chondroitin-4-sulfate].

It was shown that the mechano-structural strength and swelling of gels consisting of gelatine and potassium hyaluronate (PH) or potassium protein-chondroitin-4-sulphate (PPCS) depended on the correlation of the components. Low PH and PPCS concentrations showed a minimal mechano-structural strength and a maximal swelling of gels. In the zones of neutralization of the positive electrical charges of gelatine by macropolyanions a high mechano-structural strength coincided with a minimal swelling. With high PH concentrations the mechano-structural strength and swelling of gels became equal to those of pure gelatine; and with high PPCS concentrations there was noted an additional parallel increase in the strength and swelling of gels.

Animals↗

PubMed1976

[Different heparin fractions].

It was shown that treatment by 2-3 M KCl solutions of complex unfractionated preparations of heparin with hexamminecobalt (III) resulted in incorporation into the solution of a heparin fraction containing 3 residues of sulphuric acid per 1 residue in glucosamine. In the precipitate released of hexamminecobalt (III) there remains a heparin fraction containing 4 residues of sulphuric acid per 1 residue of glucosamine. Both fractions were isolated in a chemically individual form.

Chemical Phenomena↗

PubMed1975