Sindbis group viruses can be indirectly identified and classified. Three antigenic subtypes were distinguished by solid-phase enzyme immunoassay (SPEIA): African, West-Asian, and North-European. The negative plaque suppression test is recommended for identification of new Alphavirus strains for detecting species-specific antigenic determinants, and SPEIA for classification by appurtenance to antigenic subtypes.

Alphavirus↗

PubMedDate not supplied

[Production and study of hybridomas, producing monoclonal antibodies to the structural glycoprotein of Marburg virus].

Hybridomas producing monoclonal antibodies (MAb) to Marburg virus glycoprotein were prepared by splicing of mouse myeloma cells (NSO and X.63-Ag6.653 strains) and splenocytes of immunized BALB/c mice. Cultural and MAb-producing properties of hybridomas were studied. The production of MAb during serial passages 15-30 was confirmed. MAb titers in culture fluids were 1:4096-1:8152, in immune ascitic fluids of BALB/c mice 1:409,600-1:638,400. The possibility of using MAbs as a component of ELISA test system was demonstrated. The sensitivity of ELISA-MAb method for Marburg virus was 1 x 10(3) PFU/mu.

Animals↗

PubMedDate not supplied

[The use of monoclonal antibodies in studying the causative agents of viral hemorrhagic fevers].

The most promising trends of using monoclonal antibodies (Mabs) in virology research of the viral hemorrhagic-fevers' agents are related with studying viral antigen structure and with developing diagnostic preparations for indicating and identifying infectious agents of the mentioned pathologies. The methodological specificity of obtaining the Mabs to viral hemorrhagic-fevers' agents as well as data on its practical use are discussed.

Animals↗

PubMedDate not supplied

[Forensic medical characteristics of the injuries inflicted by home-made arbalest].

The classification of arbalests as a representative of the class of missiles is presented. Basic design characteristics of such weapon, wounds and damage to cotton targets from shot distances 1, 3, 5, 10 and 15 m are described.

Forensic Ballistics↗

PubMedDate not supplied

[Forensic medical characteristics of the injuries inflicted by modern sport bow].

Modern bows are classified as representatives of darts. Construction characteristics of bows and arrows, damage to experimental cotton targets from shooting distance of 1-15 m are described.

Forensic Ballistics↗

PubMedDate not supplied

[Tolerance to impact force distending the tissues of the neck as applied to an assessment of the conditions of highway accidents].

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Accidents, Traffic↗

PubMedDate not supplied

[Relative biological effectiveness of secondary proton radiation with energy 70 G ev depending on the criteria of its evaluation].

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Dose-Response Relationship, Radiation↗

PubMedDate not supplied

[Effect of high-energy adrons on the survival of Escherichia coli and bacteriophage T4Br+].

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Cesium Isotopes↗

PubMedDate not supplied

[Effect of secondary radiation from protons with energies of 70 Gev on the number of aberrant cells in horse bean roots].

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Chromosome Aberrations↗

PubMedDate not supplied

[Effectiveness of virus-specific proteins in immunogenesis during experimental Marburg fever].

The protective properties of early virus-specific proteins (EVSP) of Marburg virus have been studied. Immunoglobulins to EVSP in combination with immunoglobulins to UV-inactivated Marburg virus prevented lethal infection in Marburg virus infected guinea pigs. More than 80% of guinea pigs immunized by mixture of EVSP and UV-inactivated Marburg virus survived after challenge with 25 LD50 of Marburg virus. The explanation of this phenomenon and its further utilization are discussed.

Animals↗