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Biomedical subjects

V N Miliutin

Publications and source records attributed to V N Miliutin.

At least 19 recordsLinked to original sources
PubMed1987

[Immunogenicity of Yersinia pestis grown on nutrient media at 28 and 37 degrees C].

The immunogenicity of Y. pestis strain EV, grown in yeast-casein medium, yeast medium with Hottinger digest and yeast medium with sunflower-seed protein at 28 degrees C and 37 degrees C, for guinea pigs and white mice has been studied. As revealed in this study, these media ensure the formation of highly immunogenic populations of Y. pestis strain EV and, therefore, can be used for growing Y. pestis vaccine strains. Considerable fluctuations in the content of such highly protective antigen as fraction 1 do not affect the immunogenicity of live cultures of Y. pestis strain EV. This is due to the leveling of differences in the content of this antigen in the process of the multiplication of these bacteria in laboratory animals.

Animals↗
PubMed1986

[Virulence, viability and antibiotic sensitivity of the causative agent of plague growing on nutrient media at 28 and 37 degrees C].

A comparative study of virulence, viability and antibiotic sensitivity of Y. pestis strains grown at 28 degrees C and 37 degrees C in yeast-casein medium, yeast medium with Hottinger's meat digest and yeast medium with protein hydrolysate obtained from sunflower seed groats has been made. These media have been found to be suitable for the prolonged cultivation of Y. pestis at 28 degrees C and 37 degrees C, for the determination of its sensitivity to antibiotics, as well as for the preservation of Y. pestis cultures.

Animals↗
PubMed1984

[Determination of groups at risk for acute intestinal infections by a population questionnaire].

Questionnaires were distributed in 3308 families divided into two groups: group 1 with no cases of acute intestinal infections and group 2 where such cases were registered. Information contained in the questionnaires was processes by means of computers bzsm-6. The analysis of the data on the occurrence of characteristics indicating the quality of water supply and water consumption (25 characteristics), sewage and sanitation (12 characteristics), living conditions (19 characteristics) showed that the living conditions of the families in group 2 were worse than those of the families in group 1. The occurrence of these characteristics in the families of patients with acute intestinal infections and in the families of carriers were mostly the same, and the existing differences in such characteristics as "fishing", "use of water from ponds for house-hold purposes" proved to be nonessential. The population of the city was divided into four risk groups with regard to the possibility of contacting infection, depending on the conditions of water supply, water consumption and sewage in different housing areas: the group registered as stable on account of sanitary and hygienic conditions, the groups of usual, increased and maximum risk.

Acute Disease↗
PubMed1982

[NAG-vibrio serovar 2 causing a generalized infection].

A case of septicemia caused by NAG-vibrio, serovar 2, is described. In contrast to other vibrios, the strain under study was immobile at the moment of isolation and, when introduced enterally into suckling rabbits, showed the capacity of causing generalized infection.

Animals↗
PubMed1980

[Lysogenic conversion of cholera vibrios].

As a result of ultraviolet irradiation and treatment with nitrosmethyl urea, mitomycin C, ascorbic acid and sarcolysin, the elimination of prophage from wild lysogenic Vibrio cholerae strains, biotype El Tor, has been achieved. The elimination of prophage was accompanied by a change in the biological properties of the vibrios, and some of these biological properties were found to be correlated with the lysogenization of Vibrio cholerae by the definite races of temperate phages. Only a few races of type II phages, whose genome was responsible for the serological characteristics of the strain, its sensitivity to cholera diagnostic phages and polymyxin, were capable of conversion.

Ascorbic Acid↗
PubMed1979

[Preservation of cholerogenicity in cholera vibrios in the incomplete L-cycle stage].

The work presents the characteristics of the forms appearing as a result of the incomplete L cycle of Vibrio cholerae obtained in experimental conditions after the cultivation of typical V. eltor strain, serovar Ogawa, for 5 months at room temperature in sterile river water without subculturing. The culture formed shaprly changed L-similar colonies, had decreased agglutinability and was resistant to diagnostic cholera phages, but retained its cholerogenicity for suckling rabbits. A suggestion was made concerning the possible epidemiological significance of such forms and the necessity of their detection in the course of bacteriological analysis for cholera.

Animals↗
PubMed1978

[Ultrastructure of the L forms of cholera and NAG vibrios].

Ultrastructure of L-forms of cholera and NAG-vibrios obtained under the effect of penicillin, tetracycline, in prolonged cultivations, and isolated from the external environment was studied. There proved to be no principal differences in their ultrastructural organization. Cells of sphero- and protoplastic type were distinguished in the colonies. The presence in the cell cytoplasm of mesosomes of the lamellar, vesicular type, myelin-like structures, multiple ring-like formations liberated into external environment, and freely lying membranes served as the peculiarity of the L-forms under study. Budding process as a result of which there formed elemental bodies was revealed.

Cell Division↗
PubMed1975

[Electron microscopy of cholera vibrios from the intestinal contents of infected suckling rabbits].

Electron microscopic study of the cells of classic, El Tor and NAG-vibrios showed them to be no different by the structure type from the cells of ofter Gram-negative bacteria. A characteristic peculiarity of the cholera vibrios revealed after their passage through the intestine of nursling rabbits was the presence of microcapsules and protrusions of the areas of the wall membranous apparatus.

Animals↗