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Biomedical subjects

V N Sokolov

Publications and source records attributed to V N Sokolov.

At least 19 recordsLinked to original sources
PubMed1987

[Clinical x-ray characteristics of recurrent tuberculosis of the bronchopulmonary system in patients with chronic respiratory diseases].

X-ray and clinical features of tuberculosis combined with lingering pneumonia were studied in 110 patients of the 7th group of dispensary registration suffering from chronic diseases of the respiratory organs. Tuberculosis recurrences in this group of patients had a torpid course, specific changes were slowly reversed, noticeable fibrosis was formed on their site. Thorough x-ray tomographic investigation not only of the pneumonia zone but also of the zone of posttubercular changes was conducted at the onset and end of pneumonia therapy to exclude tuberculosis reactivation. Frequent exacerbations of chronic diseases of the respiratory organs including complications in the form of lingering pneumonia in patients with posttubercular changes can mask early stages of tuberculosis reactivation.

Adult↗
PubMed1978

[Changes in ATPase activity of dog kidney nuclear membrane under the effect of polyenic antibiotics].

Effect of amphotericin B and nistatin on ATPase activity of dog kidney nuclear membranes is studied in vivo and in vitro. Long-term intravenous injections of the antibiotics do not change the ATPase activity of kidney nuclear membranes. However, short-term injections of polyenic antibiotics have some effect on ATPase activity on nuclear membranes: amphotericin B, considerably activates the enzyme . In vitro incubation of isolated dog kidney nuclei with amphotericin and nistatin at concentrations of 1 and 10 mcg/ml does not affect the activity of nuclear membrane ATPase, while increased concentrations of polyenic antibiotics, (up to 200 mcg/ml) results in a slight inhibition of the enzyme activity. The role of the data obtained for solving molecular basis of the toxic effect of polyenic antibiotics.

Adenosine Triphosphatases↗
PubMed1977

[Penetration of polyene antibiotics into human embryonic kidney tissue cell cultures].

Penetration of 14C-amphotericin AM-2 into the cells of the tissue culture of the human embryon kidneys was studied by means of light autoradiography after incubation with the antibiotic. Microscopic examination of the autographs of the cell slices revealed the presence of the radioactive label in the cytoplasm and nucleoplasm of the cells. The revealed intracellular localization of the label was evident of the antibiotic penetration into the cells.

Amphotericin B↗
PubMed1977

[Distribution of polyene antibiotics in the cells of a tissue culture of puppy kidney].

The results of the studies on interacellular distribution of amphotericin AM-2, a wate soluble derivative of amphotericin in the cells of the puppy kidney tissue culture exposed to the antibiotic in a concentration of 50 gamma per 1 ml medium for 17 hours are presented. It was found that the antibiotic was firmly bound with the kidney cells: 15.4 per cent of the initial amount of the antibiotic was detected in the washed cells. Analysis of the antibiotic distribution in separate subcellular components showed that sorption of amphotericin AM-2 by the plasmic membranes, nuclei and cytoplasm was respectively 18.3, 35.6 and 9.7 per cent of the amount absorbed by the cells. Binding of a significant amount of the antibiotic absorbed by the plasmic membranes and nuclei with the above fractions was reversible. The results of the study provided a supposition that amphotericin AM-2 penetrated into the cells of the kidney tissue which adds some new findings to the studies on the mechanism of action of polyenic antibiotics in cells.

Amphotericin B↗
PubMed1975

[Study of the action of nystatin on the plasmatic membranes of the liver of rabbits].

The effect of mystatin on the plasmic membranes of the rabbit liver after intravenous administration of the antibiotic to the animals in a dose of 5 mg/kg was studied. It was found that intravenous administration of nystatin had no effect on the quantitative content of protein, lipids and nucleic acids in the plasmic membranes of the liver. The method of electrophoresis in polyacrylamide gel revealed significant changes in the composition of the liver membrane protein due to the treatment with nystatin. The effect of nystatin on the composition of lipids and fatty acids contained in the membrane lipids was also investigated. The data of the thin layer chromatography showed that nystatin did not affect the qualitative composition and the content of separate lipid fractions in the lipids of the liver plasmic membranes. However, the fatty acid analysis of the membrane lipids after intravenous administration of nystatin revealed a number of qualitative and quantitative differences in the composition of the lipid fatty acids of the membranes tested. The results showed that nystatin affected the membrane structures of the rabbit liver cells.

Animals↗
PubMedDate not supplied

[Concept of labile protein and the adaptation of nitrogen metabolism in protein deficiency in man].

Investigations involving 30 healthy males, who for 45 days consumed standard semisynthetic diets differing in the caseine content (from 0 to 15.1 g of nitrogen per day) and serving as a sole source of protein, furnished data on the nitrogen excretion kinetics and nitrogen balance. Mathematical analysis of these data allowed it to find the existence of general regularity in the nitrogen excretion and to deduce an integral equation of its losses at different levels of nitrogen consumption. Pertinent calculations have ascertained that by the labile protein one should understand not the specific proportion of the total protein in the organism, but the actual rate of its metabolism, manifesting itself in the routine lag-phase during adaptation of the nitrogen metabolism to a new level of the protein allowances in the organism. The data thus made available permitted it, at the same time, to propose a criterion for estimating the optimal requirements of man in proteins.

Adaptation, Physiological↗
PubMedDate not supplied

[Fluorographic equipment for examining the populations of regions not easily accessible].

The article describes the design of the experimental prototypes of the lightened portable collapsible photoroentgenograph and mobile photoroentgenograph on the basis of the automobile with a higher cross country travel capacity, uAz-452-A, equipped with the chamber PK-110 (GDR). The test results showed that the above photoroentgenographs are convenient and effective for the prophylactic medical examination of the population in the not easily accessible regions.

Humans↗
PubMedDate not supplied

[Planning of computerized tomography using an X-ray stereogrammetry method].

X-ray stereogrammetry is used to derive the geometry of optimum body section. Principles and methodology of a trial-and-error technique for X-ray stereogrammetric survey are analysed. Calculations of the optimum slice for one of the clinical situations are presented along with experimental results obtained with a computed tomograph.

Adult↗