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Biomedical subjects

V N Verbov

Publications and source records attributed to V N Verbov.

15 recordsLinked to original sources
PubMed1991

[An immunoenzyme method for demonstrating Coxiella burnetii antigens].

Materials on the development of an enzyme immunoassay (EIA) system for the detection of the antigens of C. burnetii, the causative agent of Q rickettsiosis, are presented. The system is highly specific and effective with respect to both corpuscular antigens of phases 1 and 2 and soluble antigen (lipopolysaccharide). The sensitivity of this method varies within the range 5-100 ng/ml. The effectiveness of EIA as a quantitative (semiquantitative) control test used in the process of the production of Coxiella preparations has been demonstrated.

Animals↗
PubMed1990

[Comparative evaluation of the immunoenzyme system used in the serodiagnosis of tuberculosis and non-tuberculous diseases].

A method for detection of antituberculous antibodies by enzyme immunoassay system developed by the authors, was tested on 317 blood serum samples taken from patients with tuberculosis and non-tuberculous diseases of the lungs, vertebral column, kidneys and genitalia. Depending on the process site, the detection rate of specific antibodies and their specificity made up 57-77% and 85-95%, respectively, which practically agrees with the data produced as a result of a simultaneously conduction of three routine serologic reactions.

Antibodies, Bacterial↗
PubMed1989

[The use of commercial immunoenzyme and latex preparations for the demonstration of human rotavirus antigens].

The results of using enzyme immunoassay and latex preparations for the diagnosis of rotavirus gastroenteritis are presented. High effectiveness of the enzyme immunoassay system developed in the USSR with latex diagnostic agents, such as Rotalex (Orion Diagnostica, Finland), Slidex Rota Kit (BioMérieux, France), The Wellcome Rotavirus Latex Kit (Wellcome Foundation Ltd., Great Britain), 48-63% and 21-41% respectively, has been noted. The results of the comparison of the system developed in the USSR with Wellcozyme Rotavirus, an enzyme immunoassay system manufactured by Wellcome Foundation Ltd. (Great Britain), are practically comparable. The results of the block test and the confirmation test used for control indicate that the Soviet preparation is specific. Materials on the practical evaluation of the assay system at health institutions are presented. Good prospects for the use of this system in the diagnosis of rotavirus gastroenteritis, as well as in the realization of epidemiological surveillance on this infection, are substantiated.

Animals↗
PubMed1986

[Use of solid-phase immunoenzyme analysis for the serodiagnosis of pseudotuberculosis].

The diagnostic test system based on the solid-phase enzyme immunoassay (EIA) for the detection of antibodies to Yersinia pseudotuberculosis in the sera of patients with the use of Soviet-made preparations and reagents has been developed. The test has been performed in microchambers for immunological reactions, thus making it possible to decrease the consumption of reagents 10-20 times in comparison with the traditional technique with the use of plates. The results of the titration of 42 sera in EIA and in the passive hemagglutination test (PHAT) are indicative of the presence of positive correlation (r = 0.78; p less than 0.05) between antibody titers in EIA and PHAT. A fourfold or greater increase in antibody titers has been determined by means of EIA in 80% of cases and with the use of PHAT in 55% of cases. The minimum diagnostic titer yielded by EIA has been determined: 1:256.

Antibodies, Bacterial↗
PubMedDate not supplied

[A modification of an immunoenzyme test system for the serodiagnosis of opisthorchiasis].

Based on the antigen derived from the whole Opisthorchis marita extraction in cats and A-peroxidase protein conjugate the authors modified the enzyme immunoassay system for the detection of IgG antibodies to the causative agent of opisthorchiasis. A vibroshaker used in all stages of study permitted them to reduce the time of the reaction and its analysis providing the visualized consideration of the results.

Animals↗
PubMedDate not supplied

[Theophylline concentration in the blood of children with persistently recurring bronchial asthma].

An antirecurrence effect of theophylline was studied in 32 children aged 3 to 12 years suffering from severe bronchial asthma. The drug dosage was determined by means of a stepwise increase of a daily dose in the range of 10-30 mg/kg/day under control of the patient's general condition and parameters of external respiratory function. Blood theophylline concentration was measured by an immunofluorescent method. The drug individual biotransformation was revealed. Euphylline proved to be a highly effective and safe antirecurrence agent in an individual selection of doses and administration regimens. Combination of theophylline with inhalation beta-adrenomimetics and corticosteroids potentiates its clinical effect without influencing biotransformation.

Aminophylline↗