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V Nĕmcová

Publications and source records attributed to V Nĕmcová.

6 recordsLinked to original sources
PubMed2000

Differences in the NADPH-diaphorase positivity of the cholinergic brain stem neurons following damage of their thalamic termination field.

Following electrolesions of the anteroventral thalamic nucleus (the target structure for the NADPH-diaphorase positive axons of the cholinergic mesopontine cells, e.g. laterodorsal, pedunculopontine and lateral parabrachial nucleus) the intensity of NADPH-diaphorase staining changed drastically, but unlikely in each nucleus: it increased in the cells of the laterodorsal nucleus on the side of the lesion and decreased on the contralateral nucleus, whereas the staining intensity of the pedunculopontine and parabrachial cells remained unchanged bilaterally. In cases with small (partial) lesions of the anteroventral thalamic nucleus only lower number of heavily stained cells were observed in the laterodorsal nucleus ipsilateral to the thalamic lesion, whereas the number of slightly stained cells in the contralateral laterodorsal nucleus decreased. In contrast, not even large lesions of other thalamic nuclei, anterior or tuberal hypothalamus, zona incerta, pallidum internum, striatum or corpus callosum changed the symmetric intensity of the NADPH-diaphorase staining of the laterodorsal, pedunculopontine or lateral parabrachial nuclei. Increased intensity of the NADPH-diaphorase staining in the cells of the mamillary body was found two weeks after the electrolesions of the ipsilateral mamillo-thalamic tract or anteromedial thalamic nucleus. No degenerative changes or decrease of number of neurons in the laterodorsal tegmental nucleus in Nissl staining were detected after the electrolesions. Our data show that electrolytic lesions of target areas can lead to an upregulation of NOS expression in the parent cell bodies, provided that there is no wide collateralization as found for the pedunculopontine or parabrachial nuclei.

Animals↗
PubMed1997

The dorsal tegmentum of the pontomesencephalic junction of the rat--immunohistochemistry (choline acetyltransferase, tyrosine hydroxylase, substance P) and NADPH-diaphorase histochemistry in frontal and horizontal sections.

37 complete frontal and horizontal series of rat brain were studied to compare the distribution of choline acetyltransferase- (ChAT), tyrosine hydroxylase- (TH), substance P- (SP), calbindin D- (Calb) and NADPH-diaphorase (NADPH-d)-positive cells within the cytoarchitectonic borders of the latero-dorsal tegmental nucleus (L-D) and its neighbourhood. We found the same distribution, number and morphology of NADPH-d-positive cells and ChAT-positive cells. Rostrally, there are no borders between NADPH-d-positive cells of L-D and NADPH-d-positive cells of the lateral part of the dorsal raphe nucleus. Only a few TH-positive cells are intermingled with ChAT/NADPH-d-positive cells at the lateral border of L-D. TH-positive cells are larger or the same size as cholinergic neutrons. Locus coeruleus and its rostral part is full of TH-positive cells and their fibres run ventromedially towards L-D. Barrington's nucleus appears in double staining (ChAT and TH or NADPH-d and TH) as an empty area bordered by ChAT- or NADPH-d-positive cells of L-D and TH-positive fibres of the locus coeruleus. Some of these fibres run through the Barrington's nucleus. The shape and size of SP-positive neurons is the same as ChAT- and NADPH-d-positive neurons. SP-positive neurons are sparsely distributed in all parts of L-D, but there are only a few SP-positive cells in its medial part. About 50% of the ChAT- and NADPH-d-positive cells are also SP-positive. Results are expressed by figures in three representative frontal sections and one horizontal section through the dorsal mesopontine tegmentum.

Animals↗
PubMed1995

Topographical organization of NADPH-diaphorase positive neurons and fibres in dorsal ponto-mesencephalic tegmentum in the rat brain.

20 complete series of the rat brain, stained with cresylviolet or with the NADPH-diaphorase positive neurons demonstration method were used to compare the topographical organization of the borders of the dorsal mesopontine tegmental nuclei (latero-dorsal tegmental nucleus, dorsal and ventral tegmental nuclei of Gudden, dorsal raphe nucleus and substantia grisea centralis pontis) and to describe in detail NADPH-d positive cells and fibres in this area. We have found that topography of the cells and the borders of the nuclei were similar by the both methods, but NADPH-d histochemistry has shown the extension of NADPH-d positive cells to neighbouring structures and we would divide some nuclei into independent parts (e.g., pars medialis, ventralis and rostralis of the laterodorsal tegmental nucleus). We discussed the possibility of the projection and configuration of NADPH-d positive fibres running from the mesopontine tegmentum to the diencephalon.

Animals↗
PubMed1995

[Phospholipase A2 in schizophrenia].

The objective of the present work was to evaluate on the basis of the authors' data and data in the literature the role of phospholipase A2 in the pathogenesis of schizophrenia. The authors submit available literature pertaining to the problem as well as their own experimental findings. Based on the submitted facts, it cannot be states that phospholipase A2 is a specific marker of schizophrenia.

Adult↗
PubMed1992

Changes in the configuration and the dimensions of the fossa hypophysealis in the rat (Rattus rattus norvegicus) dependent on the age, weight and individual size of the skull.

The aim of our work was to describe the fossa hypophysealis in the most common laboratory animal--in the rat, to measure its dimensions, to determine its stereotactic coordinates and to find the dependence of these coordinates on the age, weight and individual size of the skull in different rats. From this measurement we obtained data on the growth and possible shifts of the fossa hypophysealis but also found data facilitating the selection of animals to stereotactic operations so that the hit of the hypophysis be the most probable. There are suitable coordinates for bilaterally stereotactic intervention into the middle female rats hypophysis in conclusions of our work.

Animals↗