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Biomedical subjects

V Novak

Publications and source records attributed to V Novak.

51 records · Page 3Linked to original sources
PubMed1992

Determination of total protein in highly purified factor IX concentrates.

Protein determination by the methods of Kjeldahl, Biuret, Bradford and UV absorbance at 280 nm have been studied in regard to accuracy, precision and simplicity. A reference preparation of a highly purified factor IX concentrate, Nanotiv, reconstituted to 1/5 of ordinary volume was used in the study in order to make a comparison between the different procedures. The Kjeldahl method resulted in a protein concentration of 3.7 mg/ml, whereas the Biuret, Bradford (BSA) and UV absorbance at 280 nm resulted in protein concentrations of 3.6, 2.5 and 2.8 mg/ml, respectively. The corresponding values for specific activity were 136, 140, 200 and 179 IU/mg, respectively. These results demonstrate a great variation in the response obtained by different methods for determination of total protein.

Biuret Reaction↗
PubMed1983

[Frequency of asbestos fibers and asbestos bodies in lung sections].

100 specimens of lung resections coming from 100 patients operated on in 1980 in the thoracic surgical department were examined without any selection by turn for the presence of asbestos fibres or ferruginous asbestos bodies. Among these patients asbestos fibres were found in 65% and asbestos bodies in 46% of all cases examined. The observations were related to age, residence (town, village), occupation, and underlying diagnosis found with the surgical procedure. It could be recognized by the analysis that also the population of Slovakia is exposed to ubiquitious asbestos. An etiological relation between the frequency of asbestos fibres and the occurrence of malign tumours could not be proved. Such a relation is very complex, the cancerogenic effect of other factors (benzpyrene, smoking) has to be taken into consideration.

Adult↗
PubMedDate not supplied

High resolution MRI of the brainstem at 8 T.

A recently developed ultra high field MRI system operating at 8 T (UHFMRI) was applied for brainstem imaging. UHFMRI was performed in five healthy volunteers (three men, two women; age range 34--46 years). Sagittal and axial slices were obtained with the following settings: GE, TR 750--1,000 ms, TE 7-9 ms, FOV 20 cm, matrix 1,024 x 1,024 or 512 x 512 points, slice thickness 2 mm, resolution 195 or 390 microm/pixel. The brainstem structures were assigned based on anatomy and course. Images with good signal strength and homogeneity were acquired from the midbrain and the pons. Main intraaxial structures could be directly visualized. The periaqueductal gray matter and nuclei had higher signal intensity than the predominantly white matter structures such as the corticobulbar/corticopontine/corticospinal tracts, the sensory lemnisci, and the medial longitudinal fasciculus. Structures with high iron content such as the substantia nigra and the red nucleus were seen as prominent signal hypointensities. Numerous vessels traversing the brainstem including small perforators were also distinguished. It is concluded that UHFMRI enables the acquisition of high quality images of the upper brainstem with details approaching that of histologic specimen.

Adult↗
PubMedDate not supplied

Ultra high field MRI at 8 Tesla of subacute hemorrhagic stroke.

PURPOSE: Optimal treatment strategies and neurologic outcome after stroke depend on an accurate characterization of the lesion. There is a need for high resolution noninvasive imaging for assessment of the infarct size, perfusion, and vascular territory. MRI at the ultra high field (UHF) of 8 T offers unprecedented resolution, but its utility for stroke evaluation has not been determined yet. METHOD: A 55-year-old man with hypertension experienced sudden onset of speech arrest and right-sided hemiparesis that resolved in < 24 h with minimal neurologic deficit. MRI at 1.5 T showed initially a left posterior frontal lesion with subacute infarct (hyperintense on T2-weighted spin echo images) and right-sided frontal and periventricular lesions consistent with chronic infarct. There were many smaller white matter lesions. Delayed studies showed high signal changes involving the gray matter only on T1-weighted images. RESULTS: Gradient echo and rapid acquisition with relaxation enhancement (RARE) multislice images revealed a serpentine area of low signal in the left posterior frontal lobe gray matter suggestive of a hemorrhagic infarct, right-sided frontal lesion also showing iron deposits, multiple periventricular and cortical areas with abnormal high signal regions that were consistent with old infarcts, and numerous small vessels readily visible, more prominent on the right. CONCLUSION: MRI at 8 T displays lesions with a high resolution and striking anatomic details. Susceptibility to iron and sensitivity to detect blood products are increased at 8 T. The imaging characteristics at high field are different from those at low field, but both represent findings of iron products.

Humans↗
PubMedDate not supplied

[The HELLP syndrome--a case report].

A 31-year-old secundipara with eclampsia developed the HELLP syndrome after delivery. Clinically, along with very high blood pressure values (29.3/17.3 kPa) and eclamptic attacks, an intense pain in the upper abdomen and nausea were dominant. The patient also had severe thrombocytopenia (18 x 10(9)/L), hemolysis, and increased liver enzymes (SGOT up to 220 U/L and SGPT up to 100 U/L). An intensive therapy, including--together with usual interventions in serious EPH gestoses--also plasmapheresis, antithrombin III substitution, freshly frozen plasma, and transfusion of blood and thrombocytes, proved successful in achieving the normalization of the blood pressure, blood count and liver enzymes, as well as a clinical improvement, so that 18 days after delivery it was possible for the patient to go home, provided with necessary instructions.

Adult↗
PubMedDate not supplied

[Tubal pregnancy carried to the 27th week].

A 22-year-old primigravida in the 26/27 weeks of pregnancy with tubal pregnancy is presented. Clinical and ultrasound examinations indicated ectopic pregnancy; emergency laparotomy was performed. Intraoperatively, the tubal pregnancy with a ruptured hole was revealed. Typical salpingectomy was carried out. Histology confirmed tubal pregnancy. The postoperative course was normal.

Adult↗
PubMedDate not supplied

Working with mental distress.

Although there is a need to learn from the experience of the Allitt inquiry the notion of 'health checks' for staff is fraught with complications. Not only might it be impossible to isolate nurses who are 'unhealthy', but the measures might discourage nurses from admitting to psychological problems. Most important, the therapeutic relationship between nurses and those in their care might be threatened.

Burnout, Professional↗