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Biomedical subjects

V P Ivanov

Publications and source records attributed to V P Ivanov.

At least 19 recordsLinked to original sources
PubMed2005

The contribution of genetic and environmental factors to quantitative variability of erythrocyte membrane proteins in primary hypotension.

Our previous studies have shown that, compared with healthy individuals, patients with primary arterial hypotension (PAH) have significant quantitative changes in erythrocyte membrane proteins. The purpose of the present study was to evaluate the contribution made by genetic and environmental factors to quantitative variation of erythrocyte membrane proteins in PAH. We studied 109 hypotensive patients, 124 normotensive subjects, 222 of their first-degree relatives and 24 twin pairs by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. The decomposition of total phenotypic variance of erythrocyte membrane proteins to genetic and environmental components was performed on the basis of correlations among first-degree relatives by the least squares method. The genetic dominance and shared environmental factors were found to influence the variability of cytoskeletal membrane proteins whose contents were changed in PAH. Furthermore, variations in alpha-spectrin, actin and anion exchanger in hypotensives were substantially influenced by major gene and maternal effects. Ankyrin 2.1 and actin content was under the control of common underlying genes. Variations in membrane-associated glutathione-S-transferase and tropomyosin were predominantly affected by polygenes. These findings suggest that the putative major genes with pleiotropic effects appear to be involved in the control of quantitative disorders of erythrocyte membrane proteins in primary hypotension.

Actins↗
PubMed2005

[Polymorphism of Y-chromosomal microsatellites in Russian populations from the northern and southern Russia as exemplified by the populations of Kursk and Arkhangel'sk Oblast].

Allelic polymorphisms at five Y-chromosomal microsatellite loci (DYS19, DYS390, DYS391, DYS392, and DYS393) were typed in 87 individuals from male population samples from two geographically isolated regions (Arkhangelsk oblast and Kursk oblast) of the European part of Russia. The populations examined demonstrated substantial differences in the distribution of the DYS392 (P = 0.005) and DYS393 (P = 0.003) alleles. Estimates of genetic relationships between these populations and some other European populations (including Eastern-Slavic) showed that irrespectively of the measure of genetic distance chosen, Arkhangelsk population was closer to the populations belonging to the Finno-Ugric linguistic group (Saami and Estonians) and to the Estonian geographical neighbors, Latvians, while Kursk population was the member of a cluster formed by Eastern-Slavic populations (Russians of Novgorod oblast, Ukrainians, and Belarussians). Phylogenetic analysis of the most frequent haplotypes indicated that these differences between Kursk and Arkhangelsk populations were associated with high prevalence in the latter of major haplotypes characteristic primarily of the Finno-Ugric populations.

Alleles↗
PubMed2005

[Dynamics of echocardiography indices in different types of structural and geometric remodeling of the heart left ventricle during long-term treatment].

The author analyzed dynamics of echocardiography figures under different types structural and geometric remodeling of left ventricle of the heart during two-year treatment. 114 patients (average age 62.2 +/- 1.1) with ischemic heart disease in combination with II-III stage arterial hypertension, complicated with heart failure of class II to class II according to the classification of New York Heart Association and different cardiac arrhythmias have been examined. Long term therapy has been demonstrated to be of limited success in terms of influence on diastolic characteristics of structurally changed myocardium in patients with concentric hypertrophy of the heart. The positive effect of the treatment on contraction function of the heart of patients with eccentric hypertrophy was proved to have tendency to increase during long term treatment.

Adrenergic beta-Antagonists↗
PubMed2003

Apolipoprotein B 3'-VNTR polymorphism in Eastern European populations.

Apolipoprotein B 3' (3' ApoB) minisatellite polymorphism was studied in healthy unrelated individuals from the Russian Federation and the Republic of Belarus, in 10 populations from five ethnic groups: Russians, Byelorussians, Adygeis, Kalmyks and Yakuts. The analysis was carried out using PCR and electrophoresis followed by silver staining. Overall, 25 alleles of the 3' ApoB minisatellite, ranging from 25 to 55 repeats, were detected. Heterozygosity indices were high and varied from 0.73 to 0.84. The distributions of alleles of this minisatellite in the Caucasoid populations (Russians, Byelorussians and Adygeis) had a bimodal character, whereas that for Mongoloid populations (Kalmyks and Yakuts) had a unimodal distribution. Nei's genetic distances between the populations studied and some reference populations of Europe and Asia were estimated. Despite their allele distribution homogeneity, different East Slavonic ethnic groups were clearly resolved by multidimensional analyses. The East Slavonic and Adygei populations revealed a high similarity with European Caucasoids. The Mongoloid populations (Kalmyks and Yakuts) were considerably different from those of the European Caucasoid populations, but were similar to other Asian Mongoloid populations. The results demonstrate the variability of 3' ApoB minisatellite polymorphism not only in distant populations but also, to a certain extent, in genetically relative ones.

Alleles↗
PubMed2003

[Regulation of tonus of the autonomic nervous system in patients with frequently relapsing atrial fibrillation].

An evaluation was carried out of variability of the heart's rhythm in 269 patients with ischemic heart disease and/or hypertensive disease, their age ranging between 43 to 82 years, complicated by frequently relapsing atrial fibrillation. It is shown that in those patients presenting with frequent recurrences of arrhythmia over the course of a 24-hour period, there comes to be a considerable decline in the power of total variability of cardiac rhythm, and all spectral components and a shift in the sympathetic/parasympathetic system balance in favour of the sympathetic one. Analyzed the study were particular features of regulation of tension of the vegetative nervous system in different clinical forms of atrial fibrillation. Patients with frequently recurring atrial fibrillation have been shown to constitute rather a miscellaneous group as to patterns of the cardiac rhythm variability, which fact is to be taken account of in conducting prophylactic antiarrhythmic therapy of recurrencies of atrial fibrillation.

Adult↗
PubMed2001

Polymorphism of trinucleotide repeats in loci DM, DRPLA and SCA1 in East European populations.

A normal polymorphism at three triplet repeat loci (myotonic dystrophy (DM), dentatorubral-pallidoluysian atrophy (DRPLA) and spinocerebellar ataxia type 1 (SCA1)) were examined in healthy unrelated individuals from the Siberian Yakut (Mongoloid) population, the Adygei (Caucasian) population and nine East European populations: populations from Russia (Holmogory, Oshevensk, Kursk, Novgorod, Udmurts, Bashkir), two Ukrainian populations (Lviv and Alchevsk) and one Belarussian. The distribution of alleles for DRPLA and SCA1 were similar for all East-European populations. For the DM locus, East European populations had typical allele distribution profiles with two modes, (CTG)5 and (CTG)11-14, but some differences were found for the Bashkir population where alleles containing 11-14 CTG repeats had relatively higher frequency. The Yakut population had different allele spectra for all types of repeats studied. Higher heterozygosity levels and insignificant differences between expected and observed heterozygosity were found for all tested loci. The latter led us to suggest that the trinucleotide repeat loci analysed are not influenced by selection factors and could be useful for genetic relationship investigations in different populations.

Alleles↗
PubMed2000

[Contents of the main erythrocyte membrane proteins in patients with primary arterial hypertension and its relationship with hereditary predisposition to cardiovascular diseases].

AIM: To study quantitative content of main erythrocyte membrane proteins in patients with primary arterial hypotension and its relationship with hereditary predisposition to cardiovascular disease. MATERIAL AND METHODS: Quantitative content of main erythrocyte membrane proteins in 109 patients with primary arterial hypotension (PAH) and 124 healthy persons was measured with unidimensional polyacrylamide gel electrophoresis. Hereditary determination of PAH was studied by the clinical-genealogical method. RESULTS: PAH patients showed quantitative alterations in erythrocyte membrane protein composition: increased content of alpha-spectrin, ankyrin (band 2.1), anion exchange protein (band 3) and decreased content of actin, tropomyosin and glutathione-S-transferase. Patients with aggravated heredity for PAH had higher content of beta-spectrin and ankyrin (band 2.1 and 2.2) then patients without aggravated heredity for PAH. CONCLUSION: Quantitative alterations of membrane proteins in patients with PAH could significantly modify the structure of cytoskeleton and result in modification of the enzyme activity of transmembrane proteins (ATPases) regulating cation transport across erythrocyte membrane. Moreover, aggravated heredity for PAH predisposes to high content of cytoskeletal proteins (beta-spectrin, 2.1 and 2.2 ankyrin) which could form more compact structure of erythrocyte membrane and limit cation influx into cytoplasma.

Adolescent↗
PubMed1998

[Distribution of ABO and Rhesus phenotypes and genes in the population of Kursk region].

The distributions of AB0 and Rhesus phenotypes and the corresponding genes in the population of Kursk oblast were studied. Based on these data, genetic differentiation of rural populations with respect to the d gene frequency was revealed. The differentiation was determined by the differences in the genetic and demographic structure of these rural populations. The frequency of homozygotes for the recessive gene d and the incidence of malformations affecting the children's viability increased with an increase in the inbreeding level of a population. Genetic distances between the population of Kursk oblast and other populations were estimated.

ABO Blood-Group System↗
PubMed1998

[Population-demographic structure of the population of Kursk region: anthropometric profile of newborn children].

Based on the characteristics of body height and weight in 4905 newborns, the population-genetic structure of the rural raions (districts) of Kursk oblast (region) and in the city of Kursk was determined. An "adaptive norm area" with respect to body weight and height was distinguished for newborns in Kursk oblast. On the average, the anthropometric parameters of 20% of infants from the studied populations fell within this area. The height and growth of newborns exhibited a pronounced geographic variation and depended on the level of urbanization, as well as the sex and the health status of the newborns. In district populations, the body height and the variances of the body height and weight increased with an increase in endogamy. Therefore, the relative numbers of newborns with large values of both weight and height, as well as those with a medium body height and a small body weight, were increased. Conversely, the relative numbers of newborns with a low weight and height and with a disturbed weight-height correlation were decreased.

Anthropometry↗
PubMed1998

[Genetic-demographic structure and prevalence of congenital malformations in rural regions of the Kursk district].

Investigation of the the genetics, demography, and epidemiology of congenital malformations (CMs) in Kursk oblast revealed statistically significant correlations between population parameters and the incidence of CM in the rural rations of the oblast. The general prevalence of CM and that of the separate CM forms ("guard" CM, multiple CM, hemangioma, congenital heart disease, congenital myelocele, talipes valgus, congenital femoral luxation, polydactyly, cryptorchism) increased with an increase in the average marital age of men and women of the reproductive part of the population and with an increase in the level of the populations homozygosity (an increase in the local consanguinity and ethnic marriage assortativeness, a decrease in the populations's migration activity, an increase in the proportion of genotypes homozygous for recessive genes, etc).

Congenital Abnormalities↗
PubMed1998

[Biosynthesis of leucine aminopeptidase by Xanthomonas rubrilineans 67 in culture with various concentrations of nitrogen containing compounds].

The dynamics of consumption of amine and ammonium nitrogen and glucose in the process of Xanthomonas rubrilineans 67 growth and biosynthesis of leucine aminopeptidase was studied. It was shown that the rate of leucine, alanine and glycine consumption as a source of amine nitrogen out of 16 amino acids was the highest during the fermentation. Addition of these three amino acids or their mixtures to the medium at definite stages of the fermentation process increased the leucine aminopeptidase biosynthesis by 50 to 100 per cent. Ammonium nitrogen was not used by X.rubrilineans 67. The consumption of glucose during the fermentation was even: by the 24th hour of the process the medium contained about 10 per cent of the glucose initial concentration. The optimal temperature for the culture growth and leucine aminopeptidase biosynthesis was determined. It was shown to be 28 degrees C. Higher aeration increased the culture productivity.

Alanine↗
PubMed1998

[Distribution of family names in rural populations of Kursk region].

The coefficient of inbreeding and its components were calculated for populations of rural districts of Kursk oblast with the use of the isonymic method. The following values were obtained: F(it) = 0.000567, Fst = 0.000650, and Fis = 0.000083. The Fst value agreed with the value of Malecot's local inbreeding coefficient (0.000201). In the districts where the proportions of families with frequent family names (FFNs; a frequency of 0.001 and more) were 50% and over, these family names were sufficient to adequately describe the population structure. The migration index and the index of family-name diversity were calculated. Significant correlations were revealed between Fst, the migration index, the index of family-name diversity, Malecot's local inbreeding, the mean square distance between birthplaces of spouses, the effective migration pressure, and the coefficient of linear systematic pressure.

Biomarkers↗