The method of expert evaluation is described which was carried out to define the appurtenance of seminal spots to one of two suspected subjects. Biological sample appurtenance was identified using genetic markers by analysis of allele status of loci containing variable numbers of tandem repeats. Two loci were analyzed, ApoB and IL-6. To determine the studied loci alleles polymerase chain reaction with the DNA isolated from material evidences used as the matrix was used as well as agarose gel electrophoresis. Analysis helped determine appurtenance of seminal spots to suspected subject V.

ABO Blood-Group System↗

PubMedDate not supplied

[Use of a synthetic oligonucleotide (CAC)5 in the genomic "dactyloscopy" method].

Potentialities of a chemically synthetized oligonucleotide of a certain structure in the genomic "dactyloscopy" method were under study. A random sample analysis has demonstrated a high resolving power of this variant of the method. Arguments in favor of introducing this approach in practical forensic medical direct identification of biologic material are presented.

DNA Fingerprinting↗

PubMedDate not supplied

[Experience in determining workload standards for the forensic medical expert in a genomic fingerprinting laboratory].

Working time expenditures needed for expert evaluation of material evidences by analysis of polymorphic genome sites using polymerase chain reaction and for establishment of a doubtful parentage by genomic "dactyloscopy" method were assessed. The measurements helped establish monthly and yearly standard load, that is, the number of expert evaluations per forensic medical expert of genomic "dactyloscopy" laboratory.

DNA Fingerprinting↗