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Biomedical subjects

V P Orlov

Publications and source records attributed to V P Orlov.

14 recordsLinked to original sources
PubMed2005

Selection and characterization of phage miniantibodies to actins of different origin.

Single-chain miniantibodies (scFv's) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv's (containing 2x10(8) independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4x10(7) and 1.2x10(6) M(-1) were chosen. The isolated miniantibodies could specifically detect various plant and animal actins.

Actins↗
PubMed2003

[Role of spiral computed tomography in the assessment of the bone and soft tissue structures of the spine in the postoperative period of degenerative diseases and traumas].

The spiral computed tomography (SCT) was used in examinations of 59 patients with symptoms of neurological disorders at the postoperative period after discectomy, and 82 casualties and 24 patients with degenerative-dystrophic lesions of the spine after correcting-stabilizing operations with plasty of the vertebra with autobone, biositall-XK. An examination of this category of patients and casualties in the nearest and remote terms after operation improves the potentials of SCT-diagnostics of recurrent hernias, epidural fibrosis, spondylites, central and lateral stenoses and allows control of osteoreparation and remodelling of the spine. The improved pathogenetical treatment in these cases shortens the period of disability and does not result in persistent invalidism of the patients.

Cervical Vertebrae↗
PubMed1998

[Surgical treatment of patients with injuries of the spine of the thoracic and lumbar localization].

Surgical treatment of 276 patients with uncomplicated (71.7%) and complicated (28.3%) injuries of the spine of the thoracic and lumbar localization was taken as the material for the article. The authors propose a rational strategy and tactics of operative treatment of patients with traumas of the spine and also they make an assessment of efficiency of some new surgical technologies. The volume and optimum terms of the decompressive-stabilizing operations on the spine are determined depending on the kind of the vertebral injury and the general state of the patient. The effectiveness of the present-day systems of stabilization of the spine (transpedicular constructions and plates for the frontal fixation of the spine) was demonstrated.

Accidents, Traffic↗
PubMedDate not supplied

[Posttraumatic spinal instability and the methods for its surgical correction].

The paper deals with the outcomes of 982 patients with thoracic and lumbar spinal lesions and the data of biomechanical testing (298 studies on 103 specimens from the cervical, thoracic, and lumbar spine). Major types of acute posttraumatic spinal instability (such as axial instability, threatening fractured fragment instability, and threatening segmental instability) are defined and the stabilizing capacities of routine surgical techniques are assessed. The current systems for stabilizing the spine (transpedicular designs and plates for anterior spinal fixation is shown to be effective.

Humans↗
PubMedDate not supplied

[Circular dichroism spectral characteristics of liquid-CRYSTAL dispersions of double-stranded DNA and DNA complexes with dyes].

The circular dichroism spectra of liquid-crystalline dispersions formed of double-stranded DNA molecules were calculated. It was found that the amplitude and sing of the anomalous band in these spectra in the region of absorption of nitrous bases depend on the size of dispersed particles, the pitch of their cholesterol helix and the sign of its spatial winding. The theoretical calculations are compared with experimental data characterizing the anomalous optical properties of liquid-crystalline DNA dispersions and synthetic polynucleotides formed as a result of phase elimination in aqueous salt solutions of polyethyleneglycol. The theoretically calculated circular dichroism spectra of liquid-crystalline DNA dispersions agree with the circular dichroism spectra of these dispersions observed experimentally.

Circular Dichroism↗