India: is it plague?
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Disease Outbreaks↗
Biomedical subjects
V S Dar
Publications and source records attributed to V S Dar.
Rapid detection of rotavirus by using colloidal gold particles labeled with monoclonal antibody.
A rapid single-step, sensitive and specific immunogold assay is described for detection of rotavirus. Murine monoclonal antibody (MCA) to the group antigen of rotavirus was dotted on a nitrocellulose paper strip. This was used to capture the rotavirus antigen which was revealed using a conjugate consisting of the MCA conjugated to gold particles. This assay detects 5 x 10(7) particles of rotavirus. It also acutely detected rotavirus from fecal samples, but failed to show any reaction with microscopically confirmed fecal samples containing G. lamblia and E. histolytica. The time taken for the test was approximately 10 min. Its versatility, simplicity, sensitivity and specificity makes it useful for third world countries.
Acute Disease↗
Monoclonal anti-idiotype antibody to HSV-1 neutralizing monoclonal antibody: production and characterization.
This study is an attempt to produce and characterize murine monoclonal antibodies directed against the paratope of HSV-1 neutralizing monoclonal antibody. Monoclonal antibody 138 C5G10 which was neutralising and directed to 120 K antigen gB of HSV-1 was used as the idiotype. We were able to produce three Ab-2 monoclonal antibodies as characterized using immunofluorescence, ELISA and RIA. The findings of the present study suggest that two antiidiotypes 3AiB3E10 and 3AiB5D10 share the same unique fine specificity while 3AiB3C9 has a different specificity on 138 C5G10 paratope. The utility of such 'surrogate' antigens in serological assays and modulation of immune response is discussed.
Animals↗
Monoclonal anti-idiotypes to herpes simplex virus type 1 capable of antigen specific priming & stimulatory activity.
Anti-idiotypic antibodies (Ab-2) to HSV-1 (herpes simplex virus 1) neutralizing monoclonal antibody were raised by hybridoma. These Ab-2 were found to represent an epitope of glycoprotein B (gB-1) of this virus. To further characterise this antibody for its ability to mimic the antigenic epitope, in vitro lymphoproliferation assays were done. In this assay (i) antigen specific lymphocyte priming activity of the three monoclonal Ab-2 and (ii) the in vitro stimulating ability of these Ab-2 for gb-1 primed mouse lymphocytes were tested. We could identify two monoclonal Ab-2 which were able to prime the mouse lymphocytes in vivo. These antibodies were able to recognise the in vitro stimulation signal of the antigen gB-1 and consequently could proliferate. The stimulation index was comparable to that with the antigen. These two Ab-2 were also recognized by the antigen primed mouse lymphocytes in a specific manner.
Animals↗