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Biomedical subjects

V S Fan

Publications and source records attributed to V S Fan.

5 recordsLinked to original sources
PubMed1979

Release of membrane constituents following polyethylene glycol treatment of HEp-2 cells.

HEp-2 cell monolayers were treated with 40% polyethylene glycol for 5 min which resulted in fusion during the subsequent incubation period. A loss of cell membrane components was detected in the polyethylene glycol-treated as well as phosphate buffer/saline-treated control cells, however the polyethylene glycol-treated cells released nearly twice the amount of [14C]acetate-labeled material and [3H]glycerol-labeled lipids into culture fluids than the control cells. It was further detected that the polyethylene glycol-treated cells released only approximately half the amount of protein, glycoprotein, and glycolipid as the control cells. These results suggest that polyethylene glycol exerts a differential mode of action against cell surface components and causes the treated cells to release membrane components rich in lipids but relatively low in protein and carbohydrate-containing components.

Animals↗
PubMed1975

The cause and treatment of postvagotomy diarrhoea.

Six patients with watery postvagotomy diarrhoea and 4 patients with intractable diabetic diarrhoea were treated with cholestyramine. The diarrhoea responded completely to therapy, during which the stool content of water, sodium, postassium and magnesium fell. Two of the patients, while receiving cholestyramine, had a rise in faecal fat, but this was not accompanied by diarrhoea, the stools being well formed. Evidence is presented which suggests that the mechanism of diarrhoea is similar in both disorders and is due to division or neuropathy of the hepatic fibres of the vagus nerve. This results is distension of the gallbladder, contraction of which expels increased quantities of bile salts, which swamp the reabsorptive capacity of the small intestine and induce diarrhoea by direct action on the colon. If this theory is valid, cholecystectomy, by preventing large quantities of concentrated bile salts from suddenly entering the gut, may result in improvement of postvagotomy and diabetic diarrhoea.

Adult↗
PubMed1975

Biosynthesis of Phospholipids and neutral lipids of monkey kidney cells (LLC-MK-2) infected with Chlamydia trachomatic strain lymphogranuloma venereum (38538).

The biosynthesis of phospho-lipids and neutral lipids in normal and monkey kidney cells infected with lymphogranuloma venereum were compared using 14C-acetate and 14-C-serine in pulse-chase experiments. Synthesis of phospholipids and nutral glycerolipids were inhibited in infected cells. Phosphatidyl ethanolamine (PE) synthesis increased while phosphatidyl choline, phosphatidyl serine and cardiolipin synthesis decreased in infected cells within 36 hr after infection. Sphingomyelin syntheisis decreased after 48 hr of infection. The synthesis of PE in the infected cell followed a similar pathway found in bacteria and could be distinguished from the normal host cell. An explanation of the parsitic requirement for lipid precursors has been proposed.

Acetates↗