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Biomedical subjects

V S Gorin

Publications and source records attributed to V S Gorin.

16 recordsLinked to original sources
PubMed2003

Review: immunomodulatory activity of pregnancy-associated plasma protein-A.

This mini-review highlights the growing number of indications for the immunological importance of pregnancy-associated plasma protein-A (PAPP-A), which is a remote member of the alpha-macroglobulin plasma protein family. PAPP-A can bind a variety of cytokines and specifically cleave a binding protein for insulin-like growth factors, thereby serving as a modulator of cytokine activity. Important immune functions, such as lymphocyte proliferation response to alloantigens and lectins and expression of HLA-DR molecules are predominantly suppressed in vitro by PAPP-A. It is likely that the immunoregulatory properties of PAPP-A are very similar to that of alpha 2-macroglobulin. The experimental data allows us to suppose that PAPP-A serves to prevent the recognition of the fetus by the maternal immune system and to suppress locally the host's immune response to the tumour.

Adjuvants, Immunologic↗
PubMed1997

The effects of alpha 2-antiplasmin complex and alpha 2-antiplasmin on the secretion of IgG and IgM by cultured human mononuclear cells.

We tested alpha 2-antiplasmin (AP) and its complex with plasmin (PL) for their capacity to modulate spontaneous secretion of immunoglobulins (Ig) G and M by mononuclear cells (MNC). MNC were obtained from peripheral blood of 10 donors (5 males and 5 females) and cultured for 7 days in the presence of various concentrations of AP and AP-PL complex. The 18 hr incubation of MNC with AP-PL complex resulted in a dose-dependent increase in IgG and IgM secretion but the stimulatory effect was less significant than under conditions of incubation of MNC with the complex during a 7 day culture period. The AP-PL complex-induced stimulation occurred only in the cultures in which the dose of the complex was either close to or higher than the mean value of the complex levels in plasma of healthy donors. Elevation of IgG secretion in AP-PL complex-treated cultures from females was slightly higher than in those from males. No significant change in IgG and IgM secretion was observed in the presence of native AP.

B-Lymphocytes↗
PubMed1994

[Pregnancy-associated alpha2-glycoprotein and trophoblastic beta1-glycoprotein in the diagnosis of pyoinflammatory diseases after Cesarean section].

Blood serum pregnancy-associated alpha 2-glycoprotein and trophoblastic beta 1-glycoprotein were measured in puerperants with the normal postpartum period and with that complicated by pyo-inflammatory diseases after abdominal delivery. The levels of both proteins were found reduced in the patients with pyo-inflammatory diseases developing after cesarean section as early as on day 1 postpartum. that of alpha 2-glycoprotein by 1.2 times, that of trophoblastic beta 1-glycoprotein twofold.

Adolescent↗
PubMed1993

[Hepatocyte receptors for proteinase inhibitor complexes with plasmin].

Hepatocyte receptors have been found for such complexes as alpha 2-macroglobulin-plasmin, pregnancy-associated plasma protein A-plasmin, and pregnancy-associated alpha 2-glycoprotein-plasmin, molecular masses of these receptors being estimated. The proteinaceous component of hepatocyte membranes contains heavy chains of plasmin and a 50 kDa fragment of plasmin molecule. These chains and the fragment are able to bind the native alpha 2-antiplasmin. Using immunoblotting no hepatocyte receptors for native macroglobulins, and alpha 2-antiplasmin-plasmin, alpha 1-proteinase inhibitor were detected.

Cell Fractionation↗
PubMed1991

[Comparative study of immunosuppressive properties of pregnancy- associated protein A, alpha 2-glucoprotein and alpha 2-macroglobulin].

Effects of high-molecular proteinase inhibitors on spontaneous and mitogen-induced lymphocyte proliferation were examined in males and pregnant and non-pregnant females. All three proteins, A protein, alpha 2-glycoprotein, and alpha 2-macroglobulin were found to be potent immunosuppressors and their activity was determined by the stage of pregnancy, the dose and mode of mitogenic lymphocyte stimulation.

Culture Media↗
PubMed1991

[The effect of steroid sex hormones on the biosynthesis of alpha 2-macroglobulin and pregnancy-associated alpha 2-glycoprotein in cultures of peripheral blood mononuclear cells].

The peripheral blood mononuclears are capable of intense biosynthesis of alpha 2-macroglobulin and of weak biosynthesis of pregnancy-associated alpha 2-glycoprotein. Sex hormones of men and non-pregnant women exert no influence on the protein biosynthesis. During pregnancy alpha 2-macroglobulin biosynthesis is shortly activated, although it does not depend on the influence of sex hormones. All the steroid sex hormones provide a short-term biosynthesis of this protein during the II trimester of pregnancy, while testosteron inhibits it during the III trimester. Possible mechanisms of control of biosynthesis of these proteins are discussed.

Cells, Cultured↗
PubMed1990

[An immunoenzyme method of determining pregnancy-associated alpha 2-glycoprotein].

Methods for purification of pregnancy-associated alpha 2-glycoprotein, obtained antibodies to it, and enzyme immunoassay of this protein are described. Enzyme immunoassay permits measurement of this protein in blood serum and mononuclear cell cultures in a wide range of concentrations. The method sensitivity may be improved by removing the antibodies cross-reacting with animal proteins.

Female↗
PubMed1990

[Distribution of alpha 2-macroglobulin and pregnancy-associated protein A and alpha 2-glycoprotein in human placenta].

By means of immunoperoxidase technique staining localization in the mature placenta of a number of highly molecular proteins--alpha 2-macroglobulin and associated with pregnancy alpha 2-glycoprotein and protein-A--has been determined. These proteins are predominantly determined in syncytiotrophoblast and decidual cells. Similarity in distribution of the proteins in question is determined not only by identity of their receptors, but is evidently caused by community of their functional properties.

Decidua↗
PubMed1990

[Pregnancy-associated alpha 2-glycoproteins in the diagnosis of postpartum suppurative and septic diseases].

The levels of pregnancy-associated alpha 2-glycoprotein (alpha 2-GP) were measured in the blood sera of puerperants with physiologic postpartum and postoperative (after cesarean section) periods, and with those complicated with pyoseptic diseases. alpha 2-GP levels were changed in the blood sera of puerperants with postpartum infectious diseases. The authors discuss the possibility of predicting the postpartum complications with the use of alpha 2-GP measurements.

Adult↗
PubMedDate not supplied

[The immunohistochemical detection in the human placenta of proteins related to the blood coagulation system].

Distribution in mature human placenta of plasminogen, pregnancy-associated inhibitors of proteases (pregnancy-associated protein A (PAPP-A) and alpha 2--pregnancy-associated glycoprotein, and also of not associated with pregnancy alpha 2-macroglobulin and alpha 1-antitrypsin was examined. Primary monospecific antibodies and secondary antibodies labeled with colloid gold were used. Plasminogen was detected in the fetal and maternal blood, and also on the surface of some placental villi (as thin positively stained rims). Pregnancy-associated protease inhibitors were detected in the syncytium of the villi of all histological types and also in the fetal and maternal blood. Staining for alpha 2-macroglobulin was most intensive. This antigen was detected in the maternal and fetal blood and on the surface of the villi. alpha 2-antitrypsin was detected in the fetal and maternal blood. It has been shown that both free plasminogen and its inhibitors are retained on the surface of the placental villi.

Blood Coagulation↗
PubMedDate not supplied

[Study of structural features of macroglobulins complexed with plasmin].

Activities of plasmin and trypsin contained in complexes with alpha 2-macroglobulin, related to pregnancy alpha 2-glycoprotein and with protein A were studied in presence of various inhibitors with a molecular mass of 0.2-70.0 kDa. Specific characteristics of interaction between plasmin and these macroglobulins inhibitors as well as with alpha 1-inhibitor of proteinases, antithrombin III and alpha 2-antiplasmin were studied using electrophoresis and immunoblotting.

Blotting, Western↗