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Biomedical subjects

V S Lebedev

Publications and source records attributed to V S Lebedev.

29 records · Page 2Linked to original sources
PubMedDate not supplied

[The effect of CuCl2 on the efflux of inorganic phosphate from Escherichia coli].

The influence of CuCl2 on inorganic phosphate efflux from resting E. coli and those treated with glucose has been studied. Maintaining of high phosphate gradient on the membrane is possibly only in case of continuous supply of external metabolic energy. Treatment with CuCl2 does not lead to the increase in permeability of the resting cell membrane for phosphates, but it causes the efflux of phosphates in glucose-treated cells. The above data suggest that the efflux is determined by inhibition of energy influx into cell by CuCl2, not by damaging the cytoplasmic membrane.

Cell Membrane↗
PubMedDate not supplied

[Radiothermometric monitoring of the dynamics of wound process in the eye].

Measurement of the temperature reaction in the depth of the injured eye by the thermal contrast technique in the UHF band with the use of a radiometer permits monitoring the time course of the wound process in the eye, specify the treatment strategy, forecast the possible complications associated with the treatment and the terms of convalescence.

Adult↗
PubMedDate not supplied

[The effect of surface and membrane potential on 1-anilino-8-naphthalenesulfonate binding with E. coli membrane].

Dependence of ANS fluorescence on the surface potential of E. coli under lowered resistance of the bacterial membrane and after application of the positive diffusion potential inside the cell was investigated. It was shown that in the absence of the latter ANS binding in de-energised bacteria occurs mainly at the outside surface. It may be due to the high negative charge of the inner side of the cytoplasmic membrane. According to produced evaluation the potential of this surface is 120 +/- 25 mV. The data obtained suggest that low ANS fluorescence in intact cells is due to the membrane modification on energisation.

Anilino Naphthalenesulfonates↗
PubMedDate not supplied

[Characteristics of copper-induced streptomycin transport in Escherichia coli].

Copper dependent uptake of streptomycin by resting E. coli cells was studied. It was shown that copper stimulates the aminoglycoside uptake only when bacteria possess endogenic energy sources. Additional accumulation of positive charged molecules of the antibiotic is accompanied by partial depolarisation of the membrane, its steady state distribution between cells and the medium corresponding to the resulted value of the membrane potential. On the basis of the data obtained it was suggested that under the influence of copper membrane permeability for streptomycin increases.

Biological Transport↗
PubMedDate not supplied

[Electron spin resonance determination of copper binding site on Escherichia coli cell membrane].

The electron-spin relaxation of Escherichia coli cytoplasmic membrane strong binding Cu-centers was investigated by means of the microwave power saturation of the electronic spin resonance signal. It has been established, that copper centres in the strong binding sites of the cytoplasmic membrane E.coli may be represented in the following way: 1) isolate copper complexes with small speed of the spin-lattice relaxation; 2) isolate copper complexes with increased speed of spin-lattice relaxation by means of interaction with rapidly relaxation centres; 3) dipol-binding clasters; 4) ESR-nondetectable at T = 40 K, exchange-binding clasters, which cause increasing of the spin-lattice relaxation speed for isolate copper complexes.

Binding Sites↗