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V S Vassiliadis

Publications and source records attributed to V S Vassiliadis.

2 recordsLinked to original sources

Design of reverse osmosis (RO) water treatment networks subject to fouling.

Identifying the optimal design for an RO membrane network is not straightforward when significant fouling occurs. The most robust optimal network design will feature the minimal capital and operating costs over the anticipated lifetime of the plant, and is therefore strongly dependent on the fouling behaviour and associated mitigation. This study considers the case where the likely fouling behaviour is known and investigates how to incorporate this knowledge into the design and operation of a network. The optimisation task is complicated by the highly non-linear nature of the problem owing to membrane behaviour, fouling behaviour, network interactions and operating parameter constraints (pressures and flows). In this work, fouling is modelled as an exponential decay in membrane permeability. Three optimisation approaches are used to evaluate candidate networks: (i) laborious comparison of pre-selected individual network designs; (ii) deterministic gradient search methods, and (iii) a simulated-annealing-based hybrid stochastic-deterministic method. All of the approaches consider various configurations of a two-stage network with a maximum of three membrane units in each stage, represented in a superstructure model. The results from the approaches are compared and the most effective method for network design is discussed.

Biofilms↗

Dynamic biochemical reaction process analysis and pathway modification predictions.

Recently, the area of model predictive modification of biochemical pathways has received attention with the aim to increase the productivity of microbial systems. In this study, we present a generalization of previous work, where, using a sensitivity study over the fermentation as a dynamic system, the optimal selection of reaction steps for modification (amplification or attenuation) is determined. The influence of metabolites in the activity of enzymes has also been considered (through activation or inhibition). We further introduce a new concept in the dynamic modeling of biochemical reaction systems including a generalized continuous superstructure in which two artificial multiplicative terms are included to account for: (a) enzyme overexpression or underexpression (attenuation or amplification) for the whole enzyme pool; and (b) modification of the apparent order of a kinetic expression with respect to the concentration of a metabolite or any subset of metabolites participating in the pathway. This new formulation allows the prediction of the sensitivity of the pathway performance index (objective function) with respect to the concentration of the enzyme, as well as the interaction of the enzyme with other metabolites. Using this framework, a case study for the production of penicillin V is analyzed, obtaining the most sensitive reaction steps (or bottlenecks) and the most significant regulations of the system, due to the effect of concentration of intracellular metabolites on the activity of each enzyme.

Biochemistry↗