[Clinical pathological consultation: lung mass].
The anatomoclinical exercise consists in the discussion of the etiology of an isolated pulmonary nodule in an asymptomatic patient.
Biopsy↗
Biomedical subjects
V Segers
Publications and source records attributed to V Segers.
Does any correlation exist between the Gleason classification system and the computer-assisted microscope analysis of Feulgen-stained nuclei features in human prostate adenocarcinoma?
Grading prostatic adenocarcinomas remains an important problem. Various systems exist (including those proposed by Gleason) but none of these systems seems able to reliably predict either the lethal potential of a tumor in an individual patient or the responsiveness of an individual tumor to various forms of therapy. The most frequently used grading system, as proposed by Gleason, is essentially based on the description of tumor growth pattern. The aim of the present work is therefore to investigate whether the quantitative description of morphonuclear features (including cell anaplasia) and the DNA ploidy level can contribute significant information to the Gleason grading, thus partly at least reducing its subjective nature. This quantitative description was carried out by means of the Feulgen-stained nuclei image cytometry computation of 24 variables in 101 prostatic adenocarcinomas. The results show that both DNA ploidy- and morphonuclear-related variables were weak discriminators for the various grades of the Gleason classification system, and particularly between the high (Gleason 4 and 5) and the other Gleason-grades, i.e. the low (Gleason 1 and 2) and intermediate (Gleason 3) ones. The morphonuclear evidence of anaplasia is thus not redundant data on tumor growth pattern and may be expected to provide additional diagnostic information.
Adenocarcinoma↗
Neuronal localization in the rat brain of the messenger RNA encoding calcyphosine, a new calcium-binding protein.
The cDNA encoding calcyphosine, a new calcium-binding protein of the calmodulin superfamily which is regulated by cAMP, has been cloned in the dog thyroid (EMBO J., 8 (1989) 111-116). By in situ hybridization with synthetic oligonucleotides, we here demonstrate for the first time its neuronal localization in the rat brain. Hybridization signal was detected in all the olfactory areas; in pyramidal and non pyramidal-shaped neurons in the different layers of the cerebral cortex, especially the superficial ones; in the pyramidal cells of the different sectors of the Ammon's horn and in the granule cells of the dentate gyrus of the hippocampus; in the subiculum; in the medium-sized and large neurons of the different quadrants of the caudate-putamen and accumbens and in the cerebellar Purkinje cells. Hybridization was also observed to a lesser extent in the majority of the neurons in the basal areas of the forebrain, including septum, nucleus of the diagonal band and amygdala; in the globus pallidus, entopeduncular nucleus, substantia nigra pars reticulata and compacta, ventral tegmental area; in the subthalamic nucleus; in the thalamus; in the hypothalamus; in the brainstem and in the upper cervical spinal cord. In addition to its neuronal localization, calcyphosine mRNA was also found in ependymal cells. The non-detection of positive cells in the white matter was not in favor of prominent glial localization, although it does not exclude it.
Animals↗
Characterization of nuclear size, ploidy, DNA histogram type and proliferation index in 79 nerve sheath tumors.
The distribution values of ploidy, of the DNA histogram type, of the proliferation index and of the nuclear area are described in a series of 79 nerve sheath tumors including 7 traumatic neuromas, 55 primary and 9 recurrent benign schwannomas, 5 benign neurofibromas and 3 malignant tumors. The nuclear parameters were computed on Feulgen-stained nuclei (from archival formalin-fixed paraffin-embedded materials) by means of digital cell image analysis. The results show that 20-30% of benign nerve sheath tumors exhibit an abnormal nuclear DNA content (DNA index > 1.30). Such a feature was observed in the four groups of benign tumors, i.e. primary and recurrent schwannomas, neurofibromas and even traumatic neuromas. Aneuploid tumors exhibited triploid, hypertriploid or polymorphic DNA histogram types. The three malignant tumors under study were aneuploid. Lastly, the nuclear area and proliferation index measurements did not allow any clear distinction to be made between the four groups of benign tumors on the one hand, and between the benign and malignant tumors on the other.
Adult↗