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V Silobrcić

Publications and source records attributed to V Silobrcić.

At least 19 recordsLinked to original sources
PubMed1995

Papers published by members of the Croatian Academy of Medical Sciences, registered in international bibliographic data bases in the period 1986-1990.

Scientific productivity of the CAMS members was analyzed according to the number of papers published in periodicals covered by international Medline and Science Citation Index data bases from 1986 to 1990. Results showed the mean scientific productivity per CAMS member, and the relationship between scientific productivity and age, field of medicine or respective biomedical discipline. The pattern of scientific productivity was also found to change, when analyzed according to Medline or SCI data bases in separate.

Bibliometrics↗
PubMed1994

The effect of allogeneic presensitization on Hya-isograft survival.

The effect of allogeneic presensitization on Hya isograft survival was reinvestigated in C57BL, C3H and (C57BL x C3H)F1 females. Three weeks after transplantation of H-2 or non-H-2 differing female or male skin grafts, a syngeneic male skin graft (Hya isograft) was transplanted onto female recipients. C57BL and (C57BL x C3H)F1 females pretreated with a female skin graft of any origin rejected the C57BL male isograft as a first-set, while females pretreated with a male skin graft rejected the C57BL male isograft in a second-set manner. The same happened with the C3H male skin graft transplanted onto previously challenged (C57BL x C3H)F1 females. C3H females pretreated with a C3H or CBA female graft rejected the C3H male isograft as a first-set, whereas those pretreated with a C3H or CBA male graft rejected the male C3H isograft in a second-set manner. However, after pretreatment with a BALB/c male skin graft, C3H females were not sensitized to Hya. They rejected the C3H male isograft slightly faster than was expected for the first graft but this accelerated rejection was not specific, since it occurred as well in C3H females pretreated with grafts of BALB/c females. The best explanation for this unsuccessful immunization of C3H females is that an inferior, non-H-2-dependent Hya recognition system is involved in male isograft rejection.

Animals↗
PubMed1993

Defective phagocytosis in chronic trichophytosis.

Chronic trichophytosis as a primary clinical entity (primary chronic trichophytosis, PCT) is increasingly encountered in the literature. Its prevalent cause in Croatia is the anthropophilic form of Trichophyton interdigitale. Chronicity of the disease may result from immunological defects of the patients. Thus, in 62 patients with PCT: skin testing, enumeration of T- and B-lymphocytes in the peripheral blood, quantitation of immunoglobulin classes, and phagocytosis (random mobility, ingestion, digestion and extracellular killing) by peripheral blood leukocytes was tested. The findings were compared to those in healthy persons. Defective phagocytosis (random mobility, ingestion and digestion) was found in patients with PCT (P < 0.001). All the other results were within the control range. Therefore, PCT seems to be associated with defective phagocytosis of peripheral blood leukocytes in affected persons.

B-Lymphocytes↗
PubMed1992

Phagocytic function in patients with recurrent urinary tract infections.

Phagocytes play an essential role in the host's defence against uropathogenic bacteria which are mostly extracellular pathogens. The functional capacity of peripheral blood phagocytes (predominantly polymorphonuclears, PMN) was investigated in 47 patients (urethrocystitis, acute or chronic pyelonephritis) and in healthy persons. The random mobility of phagocytes was determined by measuring their spontaneous migration from a capillary tube. Using radioactively labelled sheep erythrocytes as targets and the phagocytes as effector cells, ingestion, digestion and extracellular cytotoxicity were determined. All the four phagocytic functions in patients were significantly lower than in healthy controls, especially in patients with chronic pyelonephritis. These results link reduced phagocytosis by blood phagocytes with recurrent urinary tract infections (UTI). Whether the defect is primary or secondary to infection (and only transient) should be the object of further studies.

Adult↗
PubMed1988

A peptidoglycan monomer as an antitumor agent in mice: stimulation of phagocytosis by resident peritoneal macrophages with peptidoglycan monomer.

Peptidoglycan monomer (dissacharide-pentapeptide, PGM) from Brevibacterium divaricatum is an immunomodulator and an antitumor agent. As part of our investigation of the antitumor properties of PGM in mice, its potential to stimulate phagocytosis by resident peritoneal macrophages was assessed. Inbred CBA/H/Zg tau mice (kept under conventional conditions) were used, and a simple and brief method was developed to evaluate phagocytosis. It consisted of a short (10 min) coincubation of yeast cells (YCs) with peritoneal cells (PCs) and microscopic (phase-contrast) scoring of YC ingestion. Three samples of PGM were injected intravenously into mice (60 mg/kg), and PCs were collected from groups of recipients 8, 16, 24, 48, or 72 h later. All samples temporarily increased phagocytosis, but the extent and duration of the effect varied. Stimulation of phagocytosis also occurred after in vitro exposure (3 h) of PCs to PGM, or to the pentapeptide (PP) part of its molecule. We concluded that PGM could enhance phagocytosis by resident peritoneal macrophages in vivo and in vitro, the PP being the active component in vitro.

Animals↗
PubMed1983

Relation between reduced electrophoretic mobility of human lymphocytes and the inhibition of spreading of human monocytes caused by the same anti-human lymphocyte globulin.

Slowing of electrophoretic mobility of human peripheral blood lymphocytes after adding anti-human lymphocyte globulin (AHLG) in vitro correlated with indices of the monocyte spreading inhibition obtained by the same AHLG. Consequently, electrophoretic results may be associated with the prolongation of skin allograft survival in primates - the best and the only practical in vivo test for determination of the immunosuppressive potency of AHLG. If so, the simple electrophoretic test could be used in combination with other in vitro tests, preferably for monitoring of the AHLG production.

Antilymphocyte Serum↗
PubMed1978

Chimaerism in lymph nodes of F1 into irradiated parental recipient chimaeras rejecting skin allografts from the other parent.

Mice of the C57BL strain were irradiated with 800 R over the whole body. The next day they received i.v. a mixture of 50 x 10(6) spleen and bone marrow cells from (C57BL x CBA-T6T6)F1 hybrid mice, and were challenged with CBA-T6T6 skin grafts later on. About 20% of the recipients rejected the CBA-T6T6 skin, whereas the others were completely tolerant for more than 200 days. By using the cytotoxic test, we found that both tolerant and nontolerant recipients were complete chimaeras, i.e., had only (C57BL x CBA-T6T6)F1 cells in their lymph nodes. However, analysis of the same mice by the chromosome marker technique disclosed a proportion of host (C57BL) cells in lymph nodes of both tolerant and nontolerant chimaeras. The percentage of host metaphases in nontolerant chimeras was significantly higher than that in tolerant chimaeras (P less than 0.01). It is possible therefore that the CBA-T6T6 skin grafts were rejected by residual host (C57BL) cells rather than (C57BL x CBA-T6T6)F1 cells reacting against skin-specific transplantation antigen(s) of the parental graft.

Animals↗
PubMed1976

Acute graft-versus-host reaction in mice. 3. Organ distribution of injected 51 chromium labeled lymphocytes.

The distribution of labeled lymph node cells, causing an acute GvH reaction in lethally irradiated allogeneic recipients, was studied. Lymph node cells of C57BL mice were labeled with 51Cr and injected into lethally irradiated: a) C57BL mice, b) CBA mice, c) CBA mice sensitized to C57BL antigens prior to irradiation, d) CBA mice splenectomized before irradiation. Two more experimental situations were studied in which C57BL donors of lymph node cells were: e) presensitized to CBA antigens, or f) deprived of T-lymphocytes. The amount of radioactivity was determined in the whole body, blood, liver, spleen, subcutaneous lymph nodes, lungs, femora and kidneys of the irradiated recipient at regular intervals from the time of injection to the 120th hour after it. We found that living cells lodged predominantly in the spleen and the lymph nodes, while dead and dying cells accumulated in the liver. Other organs contained very small amounts of radioactivity. All the results point to the primary role of the spleen in the acute graft-versus-host reaction.

Acute Disease↗
PubMed1976

Acute graft-versus-host reaction in mice. I. Cellular events.

Cellular events in the spleen during the development of acute GvH reaction in lethally irradiated recipients of allogeneic lymphocytes may be divided into 6 interrelated processes: (1) entering and lodging in the spleen of about 17% of inoculated cells which forms the compartment of potentially reactive cells; (2) transformation (recruitment) of about 30% of cells lodged in the spleen into large pyroninophilic cells (LPC), a process lasting about 12-16 hr; (3) proliferation of recruited LPC by five successive divisions with a Tc of about 12 hr; (4) transformation of LPC into non-LPC; (5) proliferation of non-LPC by one division, with a Tc of about 8 hr; and (6) migration of mature immunologically active cells from the spleen. The last process correlates well with the concomitant appearance of lymphocytes in the peripheral blood (killer cells) and with the time of acute death among inoculated mice.

Animals↗
PubMed1976

Inhibition of human monocyte spreading. An in vitro test for immunosuppressive potency of antihum lymphocyte globulin.

Monocytes from human peripheral blood, when incubated in vitro, spread onto the surface of the glass. Horse antihuman lymphocyte globulin (ALG) added to the incubation chamber inhibits the spreading, while normal horse globulin (NHG) does not. The inhibition depends on the concentration of ALG admixed to the mononuclear blood cells. Eleven coded samples of antihuman ALG were assayed for the ability to inhibit monocyte spreading. This potential was then compared to the in vivo immunosuppressive effect of the same samples determined by the prolongation of skin allograft survival in subhuman primates. It was found that the in vitro inhibitory acttivity correlated rather well with the in vivo immunosuppression, Therefore, the inhibition of monocyte spreading is proposed as an additional test system for the in vitro evaluation of the immunosuppressive potential of antihuman ALG.

Animals↗