Biomedical subjects

V Strnadová

Publications and source records attributed to V Strnadová.

At least 19 recordsLinked to original sources

PubMed2006

[Prophylactic administration of homoisoflavonoid in ischemic-reperfusion damage of the renal tissue in the laboratory rat].

The study aimed to examine the antioxidizing effect of homoisoflavonoid in prophylactic administration under the conditions of renal ischemia-reperfusion in the laboratory rat. The pathological model for the in vivo experiment was unilateral renal ischemia-reperfusion of the laboratory rat. The animals were randomized into 5 groups. Homoisoflavonoid was administered to treated groups orally in doses of 5, 10 and 20 mg/kg once a day in 0.5% Avicel solution. The placebo group received Avicel only, and the intact group was without medication and intervention. On day 15 of the experiment, renal tissue ischemia/reperfusion (60/10 mins) was induced in the treated and placebo groups. Then the animals were exsanguinated, biochemical parameters in the blood (superoxidismutase, glutathionperoxidase, total antioxidizing capacity and malondialdehyde) were assayed, and renal samples were withdrawn for histopathological examination. A biochemical examination demonstrated a dependence of the effect of homoisoflavonoid on the dose administered. An obvious effect was demonstrated in the values of GSHPx, AOC, and MDA. On the other hand, a negative dependence was found between the dose of administered homoisoflavonoid and SOD and GSHPx values. The results of biochemical examination correlate with the histopathological pictures of the renal tissue and support the assumption about a protective effect of homoisoflavonoid under the conditions of artificially induced pathological state--renal tissue ischemia-reperfusion.

Animals↗

PubMed2006

[Cardiovascular diseases and gene polymorphisms].

Cardiovascular diseases rank among the most important mortality causes in the Czech population. Although the unhealthy living style plays a key role in their development and progression, impacts of the hereditary predisposition can not be denied. This article characterizes the gene polymorphisms and results of mutations in general. An overview of the most important findings presented in papers published in the Czech Republic and worldwide follows. Issues of concrete gene polymorphism associations with such pathological conditions as acute or chronic heart failure, essential hypertension or heart rhythm disorders are discussed. Attention is paid to genes that participate on beta-adrenergic signalling, to genes for angiotensin, to ACE-gene and to angiotensin II receptor gene. A table summarizing the most important data is attached to the article.

Angiotensinogen↗

PubMed2004

Response of saprotrophic microfungi degrading the fulvic fraction of soil organic matter to different N fertilization intensities, different plant species cover and elevated atmospheric CO2 concentration.

The response of the cenosis composition of soil saprotrophic microfungi able to utilize the fulvic fraction of soil organic matter to increased concentration of atmospheric carbon dioxide, plant species cover quality and different levels of nitrogen fertilization was determined under field conditions in a free-air carbon dioxide enrichment experiment. Twenty-nine species of microfungi were isolated from the tested soil. The effects of CO2 enrichment and plant species cover were not significant. Nitrogen fertilization was identified as the only significant factor inducing changes in the abundance of soil microorganisms. This was reflected in a relatively low value of quantitative Sørensen similarity index on comparing fertilized and unfertilized treatments and in 2-way ANOVA of total CFU counts. Some differences were observed in species diversity between the two variants of all treatments. No association between microfungi and the factors under study was found by using the Monte Carlo Permutation test in redundancy analysis.

Base Sequence↗

PubMed2002

[Effect of Consupren, Carvedilol and BL-443 on the composition of lactate dehydrogenase in tissues of rats with cyclosporin nephropathy].

L-Lactate dehydrogenase pattern in tissues was determined by polyacrylamide gel electrophoresis adjusted to be more sensitive to LD1 and LD2. Three groups of rats (Wistar) were treated for 18 days with single oral daily doses of 45 mg consuprene/kg body weight to induce cyclosporine nephropathy. Two treated groups were further medicated either with carvedilol or BL-443 in single daily doses of 10 mg/kg b.w., unmedicated rats were given single i.p. daily doses of 1 ml saline. No significant difference in the LD(1-4) isoenzyme pattern in the liver between intact rats, rats with cyclosporine nephropathy, and rats with cyclosporine nephropathy medicated with carvedilol or BL-443 was found by F-test and t-test (p < 0.05). However, a significant difference in the LD(1-4) isoenzyme pattern in the myocardium between rats with cyclosporine nephropathy and intact rats was found. The present study reports the preliminary results of the effects of consupren on the LD(1-4) pattern in the muscle, spleen, and lung, as well as the effects of carvedilol and BL-443 in the tissues under the conditions of experimental cyclosporine nephropathy. The tissues with an increased risk of affection can be identified by evaluation of LD patterns that may become an additional tool to microscopic examination of the sample.

Adrenergic Antagonists↗

PubMed2002

[Monitoring cardiotoxicity of anthracyclines in children and possibilities of its prevention].

UNLABELLED: Chemotherapy is the basic therapeutic method in pediatric oncology. Encouraging results of treatment of tumours in children may be adversely influenced by late side effects of cytostatics. Most serious is the late cardiotoxicity of anthracyclines. The authors analyzed and evaluated in a retrospective study clinical and laboratory findings in patients treated during childhood on account of malignity by chemotherapy containing anthracyclines, and focused their attention on late toxicity and evaluation of the effect of cardioprotection of dexrazoxane (ICRF-187, Cardioxane). The investigation comprised 73 patients aged 15 +/- 4.7 years who were given a cumulative dose of anthracycline (doxorubicin or daunorubicin) 244 +/- 13 mg/m2 and were in long-term remission of the disease. The mean follow up period after terminated chemotherapy was 6.7 +/- 3.4 years. Cardioprotection was administered to 42%, to 58% it was not administered. The authors did not find a significant difference in the results of anthropometric, haematological and biochemical examinations in the two sub-groups. A decrease of the left ventricular ejection fraction below 50% was diagnosed in 4 patients (5% of the group) whereby two developed cardiomyopathies with the clinical picture of heart failure and one patient died due to progressive heart failure. Cardiotoxicity was diagnosed only in the sub-group without cardioprotection (9.5% of the sub-group). In patients without cardiotoxicity in the investigated sub-groups no significant difference was found in the left ventricular ejection fraction at rest or after a stress (in dynamic stress echocardiography), in the tolerance of a stress and circulatory indicators. A significant and relatively close negative relationship was found between the administered cumulative dose of anthracyclines and the left ventricular ejection fraction at rest (r = -0.62, p < 0.001) and after a stress (r = -0.64, p < 0.001). CONCLUSION: The finding of a 5% incidence of cardiac damage of the myocardium in the whole group after a relatively short period after termination of chemotherapy is sufficient reason for long-term cardiological and in particular echocardiographic follow up of patients treated in childhood on account of a malignity with anthracyclines and for a rational approach to the administration of cardioprotection.

Adolescent↗

PubMed1997

Dairy cattle as a bioindicator of exposure to genotoxic substances in a heavily polluted area in northern Bohemia.

The exposure of dairy cattle to genotoxic agents in two districts with different levels of environmental pollution was estimated using cytogenetic analysis of bovine peripheral lymphocytes. The Teplice district represented an industrialized area where the air pollution rate is extremely high mainly in the winter, and the Prachatice district--an agricultural area with a relatively low level of pollution. The Ames test was used to examine feed samples for the content of mutagenic substances. Cows in the Teplice district showed a significantly higher count of aberrant cells (4.83 +/- 2.36) than cows in the Prachatice district (3.63 +/- 2.12). The sum of revertants induced by rinsings or extracts of feeds in both of the two test strains (Salmonella typhimurium TA 98 and TA 100) was significantly higher in the district of Teplice than in the district of Prachatice. The percentages of findings with mutagenic responses were 56.3 and 34.8% for the districts of Teplice and Prachatice, respectively. No mutagenic activity was found in milk samples collected in any of the districts. Apparently, the cows kept in the Teplice district were more exposed to genotoxic substances than the cows in the Prachatice district. The major source of this exposure was probably fresh fodder contaminated by industrial emissions.

Air Pollutants↗

PubMed1996

Mutagenicity of feeds and bovine milk in districts with different levels of environmental pollution.

Mutagenic activities of feedstuffs and bovine milk were investigated by the Ames test in two districts of the Czech Republic (Teplice and Prachatice), differing in the levels of industrial pollution. Salmonella typhimurium strains TA 98 and TA 100 with or without metabolic activation were used in the tests. Samples of currently fed feedstuffs and milk were collected in both districts in the spring 1992, autumn 1992, spring 1993, and autumn 1993. The Teplice and Prachatice sets included 12 and 11 representative samples, respectively. The samples were rinsed and extracted with acetone and methanol. Thus each sample was divided into four separate samples. The total number of revertants obtained in the industrial district Teplice was significantly higher than the corresponding value for the Prachatice district. Some between-the-seasons differences were also found within each of the two districts. The strain TA 98 proved to be more sensitive in the mutagenicity tests than the strain TA 100. Thirty-eight and thirty-one milk samples were collected in the districts Teplice and Prachatice, respectively. The overall evaluation demonstrated a significant between-the-districts difference in the total number of revertants induced by the milk samples in the strains TA 98 and TA 100.

Animal Feed↗

PubMed1995

Mutagenicity of stable dust and drinking water on swine and cattle farms.

Single pilot examinations of mutagenicity of stable dust and drinking water were made on three swine farms (D., M., T.) and one cattle farm (N.) in the district of Hodonín in summer 1994. The mutagenicity was examined by the Ames test using the indicator strains Salmonella typhimurium TA 98 and TA 100 with (+S9) or without (-S9) metabolic activation. At the same time the contents of selected pesticides (PES) and polychlorinated biphenyls (PCB) in stable dust and drinking water and that of polycyclic aromatic hydrocarbons in stable dust were determined. Increased mutagenicity was demonstrated in drinking water (strain TA 98 with metabolic activation; index Rt/Rk 3.6-7.7) and stable dust (strain TA 100 with metabolic activation; index Rt/Rk 2.2) collected on the swine farm M. High contents of PAH (8.246 mg/kg) and PCB (0.263 mg/kg) were also found in the dust samples collected on this farm. Only drinking water showed mutagenic activity (strain TA 98 without metabolic activation; index Rt/Rk 2.6) on the swine farm D. On both the farms, the number of revertants was dose-dependent. Increased content of PAH (2.553 mg/kg) was also demonstrated on the dust samples collected on the farm D. No significant increase (twofold or higher when compared with negative controls) of mutagenic activity of stable dust or drinking water was demonstrable on the swine farm T. and the cattle farm N. Substances responsible for the mutagenicity of drinking water on the farms D. and M. have not yet been identified. Anyway, the increase of mutagenicity of stable dust and drinking water should be taken as a warning that mutagens that can jeopardise animal and human health have penetrated into the stable environment.

Animals↗

PubMed1992

[Creatine phosphate as a drug in acute myocardial infarct].

The reserves of endogenous creatine phosphate are very rapidly exhausted in ischaemia. This leads at first to reversible and later to irreversible cell damage. Creatine phosphate can be administered in the preparation Neoton. It serves not only as a source of energy after arrest of oxidative phosphorylation; its main effect is stabilization of sarcolemma and protection against its disintegration. Several publications provide evidence of its favourable effect on the protection of the heart muscle during operations on the open heart, on a reduced incidence of ventricular arrhythmias in acute myocardial infarction and improvement in chronic cardiac insufficiencies. In Czechoslovakia multicentre clinical studies are under way testing Neoton in cardiological indications.

Animals↗

PubMed1990

Intracisternal administration of Mycobacterium tuberculosis potentiates the delayed type hypersensitivity in guinea-pigs.

The delayed type hypersensitivity (DTH) was studied in guinea-pigs using the skin test. The mycobacterium tuberculosis (M. tbc)--was applied by various routes. The control group received ovalbumin in Freund's incomplete adjuvant (FIA) into the footpad. The first experimental group received ovalbumin in Freund's complete adjuvant (FCA) into the footpad. The other experimental groups always received, in addition to ovalbumin plus FIA into the footpad, the M. tbc. 1. intracisternally, 2. intramuscularly, 3. intraperitoneally, 4. orally. On the day of administration of the sensibilizing substance, the body temperature was monitored. The skin test was measured after 14 and 21 days. It was established that, for the study of the DTH, the 21-day interval was more significant than the 14-day interval. A 100 times smaller dose of M. tbc. given intracisternally had the same immunostimulating effect as the injection of ovalbumin with M. tbc into the footpad (p less than 0.01). The size of the skin reaction was not only significantly influenced by the intramuscular and oral administration of M. tbc. On the other hand, the intraperitoneal administration inhibited the DTH (p less than 0.01). The increase of body temperature after the administration of M. tbc. correlated with the influence on the DTH except for the intraperitoneal administration. The route of the M. tbc. administration was crucial for the development of the DTH.

Animals↗

PubMed1987

Long-term follow-up EEG studies in patients with traumatic apallic syndrome.

The report deals with the changes of brain functions in the course of traumatic apallic syndrome (TAS) by means of the EEG examination, in the classical way, and by computer analysis as well. The development of EEG changes during individual stages of the TAS is studied. The EEG examination was performed repeatedly in 35 patients from the first days up to several years after injury. As for EEG findings as a possible prognostic index, the authors find that a gradual increase of frequency from delta to theta and alpha zones can be considered a favourable prognostic.

Brain Injuries↗

PubMed1987