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V Urbain

Publications and source records attributed to V Urbain.

8 recordsLinked to original sources

Optimisation of Noosa BNR plant to improve performance and reduce operating costs.

Noosa WWTP is publicly owned and privately operated by Australian Water Services. The process includes primary sedimentation, raw sludge fermentation, biological nutrient removal (BNR), sand filtration and ultraviolet (UV) disinfection. An innovative feature of the plant is the supplementary carbon dosing facility to avoid the use of metal salts (alum or ferric) for phosphorus removal. The average flow treated during 2000 was 9.0 ML/d. The annual 50 percentile effluent quality requirements for nutrients are total N < 5 mg/L and total P < 1 mg/L. The objectives of this project were to: determine the cause of variability in phosphorus removal; develop a strategy to control the variability in phosphorus removal; and minimise the operating cost of supplementary carbon dosing while achieving the effluent quality requirements. An investigation of chemical and microbiological parameters was implemented and it was concluded that there were several factors causing variability in phosphorus removal, rather than a single cause. The following four major causes were identified, and the control strategies that were adopted resulted in the plant achieving annual 50 percentile effluent total P = 0.37 mg/L and total N = 3.0 mg/L during 2001. First, phosphorus removal was limited by the available VFA supply due to consumption of VFA by other organisms competing with phosphate accumulating organisms (PAO), and due to diurnal variations in the sewage VFA and phosphate concentrations. Therefore, supplementary carbon dosing was essential to make allowance for competing reactions. Second, increasing the fermenter VFA yield via supplementary carbon dosing with molasses was found to be an effective and economic way of ensuring reliable phosphorus removal. Third, nitrate in the RAS resulted in consumption of VFA by denitrifying bacteria, particularly with process configurations where the RAS was recycled directly into the anaerobic zone. Incorporating a RAS denitrification zone into the process rectified this problem. Finally, glycogen accumulating organisms (GAO) were observed in BNR sludge samples, and consumption of VFA by GAO appeared to cause decreased phosphorus removal. Better phosphorus removal was obtained using VFA derived from the fermenter than dosing an equivalent amount of acetic acid. It was hypothesized that GAO have a competitive advantage to use acetate and PAO have a competitive advantage to use propionate, butyrate or some other soluble COD compound in the fermenter effluent. Contrary to popular belief, acetate may not be the optimum VFA for biological phosphorus removal. The competition between PAO and GAO for different VFA species under anaerobic conditions requires further investigation in order to control the growth of GAO and thereby improve reliability of biological phosphorus removal processes.

Bioreactors↗

Nitrogen removal in a SBR using the OGAR process control system.

OGAR is an industrial process control system that utilises on-line redox measurements to control the aeration sequence in an activated sludge process. Compared to conventional process control systems that use dissolved oxygen, OGAR makes use of redox as a control parameter during both aerobic and anoxic conditions. This paper reports on its first application in a sequencing batch reactor. The principal aim of this project is to demonstrate the capability of OGAR to achieve concentrations of total nitrogen in the treated effluent of less than 10 mg/L, which are typically stipulated by Queensland's Environmental Protection Agency (EPA). In this study the use of the control system resulted in the following SBR performance: consistent effluent total nitrogen less than 5.0 mg/L; ammonia concentration reduced from 31.6 mg/L to 0.32 mg/L, effluent nitrate 2.8 mg/L; DO setpoint 1.5 mg/L had 10% higher effluent total nitrogen compared to DO setpoint 4.0 mg/L and redox end-points for nitrification 400 mV, denitrification 150 mV.

Ammonia↗

Application of COD fractionation by a new combined technique: comparison of various wastewaters and sources of variability.

A new respirometric method for COD fractionation was applied to various wastewaters collected on French treatment plants. Great variations were observed especially in the readily biodegradable fraction (RBCOD) ranging from 1 to 16% of total COD. Variability of the results among the origin can be explained by the reactions occurring in the sewers. In one of the towns studied, the wastewater samples collected directly in the sewer show COD characteristics very different from the sewage which reaches the treatment plant. By analysing the same wastewater after aerobic and anaerobic storage, it was proved that RBCOD respectively decreased or increased significantly. Seasonal fluctuations were also quantified, showing that RBCOD fraction decreased from 75 mg/L to -15 mg/L during the summer whereas the variation of soluble fraction was less important.

Bacteria, Aerobic↗

Performance of the full-scale biological nutrient removal plant at Noosa in Queensland, Australia: nutrient removal and disinfection.

Stringent effluent quality guidelines are progressively implemented in coastal and sensitive areas in Australia. Biological Nutrient Removal (BNR) plants are becoming a standard often including a tertiary treatment for disinfection. The BNR plant in Noosa - Queensland is designed to produce a treated effluent with less than 5 mg/l of BOD5, 5 mg/l of total nitrogen, 1 mg/l of total phosphorus, 5 mg/l of suspended solids and total coliforms of less than 10/100 ml. A flexible multi-stage biological process with a prefermentation stage, followed by sand filtration and UV disinfection was implemented to achieve this level of treatment. Acetic acid is added for phosphorus removal because: i) the volatile fatty acids (VFA) concentration in raw wastewater varies a lot, and ii) the prefermenter had to be turned off due to odor problems on the primary sedimentation tanks. An endogenous anoxic zone was added to the process to further reduce the nitrate concentration. This resulted in some secondary P-release events, a situation that happens when low nitrate and low phosphorus objectives are targeted. Long-term performance data and specific results on nitrogen removal and disinfection are presented in this paper.

Acetic Acid↗

DNA extraction from activated sludges.

To optimize the cell lysis step for DNA extraction from activated sludge samples, two floc dispersion methods (sonication versus stirring with a cation exchange resin), and three cell lysis treatments (lysozyme + SDS, sonication in a water bath, and thermal shock) were tested. For dispersion, stirring with cation exchange resin was more efficient than sonication. The cell lysis procedures were applied in two sequences, and DNA was quantified after each cell lysis treatment. Lysozyme + SDS was the most effective step in the cell lysis procedures. The cell lysis treatment sequences giving the highest DNA yields were not the same for all the sludges. The differences in sludge microbial compositions and floc structures required specifically adapted cell lysis protocols. The proposed protocols were highly efficient for DNA extraction, yielding about 50 mg DNA g-1 volatile suspended solids, and allowed PCR amplification of 16S rDNA.

Bacteria↗

Estimation of nitrifying bacterial activities by measuring oxygen uptake in the presence of the metabolic inhibitors allylthiourea and azide

The effects of two metabolic inhibitors on an enriched nitrifying biomass during incubation for short periods of time were investigated by determining respirometric measurements. Allylthiourea (86 &mgr;M) and azide (24 &mgr;M) were shown to be strong, selective inhibitors of ammonia and nitrite oxidation, respectively. Consequently, a differential respirometry method for estimating nitrifying and heterotrophic bacterial activities within a mixed biomass is proposed.

Journal Article↗

Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria.

A hierarchical set of five 16S rRNA-targeted oligonucleotide DNA probes for phylogenetically defined groups of autotrophic ammonia- and nitrite-oxidizing bacteria was developed for environmental and determinative studies. Hybridization conditions were established for each probe by using temperature dissociation profiles of target and closely related nontarget organisms to document specificity. Environmental application was demonstrated by quantitative slot blot hybridization and whole-cell hybridization of nitrifying activated sludge and biofilm samples. Results obtained with both techniques suggested the occurrence of novel populations of ammonia oxidizers. In situ hybridization experiments revealed that Nitrobacter and Nitrosomonas species occurred in clusters and frequently were in contact with each other within sludge flocs.

Ammonia↗