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V V Antonenko

Publications and source records attributed to V V Antonenko.

8 recordsLinked to original sources
PubMed1987

[Synthesis and immunogenic properties of peptides corresponding to the 200-210 site of the oncoprotein p28sis].

Synthesis of peptides corresponding to the 201-210 and 200-206 sequences of p28sis oncoprotein is reported. This region of the oncoprotein was chosen as a probable epitope basing on the analysis of its secondary structure and hydrophilicity profile. The synthesis was carried out by classical method in solution using side-protecting groups of the benzyl type. Conjugates of the peptides with protein carriers and polyclonal antibodies to the peptides were obtained. Antiserum against the 201-210 peptide conjugate is shown to be specific to p28sis oncoprotein and its p56sis dimer.

Amino Acid Sequence↗
PubMedDate not supplied

Comparative study of methods to couple hindered peptides.

A comparative study of modern coupling reactions involving Boc-protected amino acid derivatives and dipeptides with N-terminal alpha,alpha-dialkylation and N-methylation was carried out. The coupling reactions were run using either equimolar amounts of the amino and activated carboxyl components or an excess of the activated carboxyl component. Yields of the target tripeptide Boc-Phe-Xaa-Phe-OBzl (Xaa = (NMe)Ala, (NMe)Aib, or (NMe) alpha Ac5c) were compared. Less than 10% of the product was obtained from methods utilizing pivaloyl mixed anhydride, pentafluorophenyl ester or acyl fluoride activation when Xaa = (NMe)Aib and (NMe) alpha Ac5c. At room temperature, significant yields of these two products were obtained from reactions which utilized an excess of the HBTU reagent (O-benzotriazol-1-yl-N,N,N',N'-tetramethyluronium hexafluorophosphate), the PyBroP reagent (bromo-tris-pyrrolidino-phosphonium hexafluorophosphate) or Boc-Phe-NCA (Boc-protected phenylalanine N-carboxyanhydride). Moreover, the Boc-Phe-NCA method was superior when used over a prolonged reaction time or at elevated temperature.

Amino Acid Sequence↗
PubMedDate not supplied

[Tumor cell proteins, detected using antibodies to the S- and N-terminal fragments of the fos proto-oncogene product].

Antibodies to c-fos oncoprotein were produced in rabbits by immunization with synthetic peptides, corresponding to the sequences 6-15 of N-end and 371-380 of C-end of c-fos oncoprotein. C-fos expression was tested with immunoprecipitation and immunoblotting in various transformed cell lines with antibodies to N- and C-decapeptides. It was shown that antibodies to C-terminal decapeptide revealed a c-fos gene product and also some fos-related antigens FRAs 36 kD, 46 kD, 75 kD and 90 kD in rat pheochromocytoma PC-12 cells and mouse carcinoma cell lines MAC-3 and LL. In some cell lines 46 kD FRA was expressed in the absence of p62 c-fos. Besides, different clones of the same cell line cultivated in identical conditions revealed differences in the 46 kD FRA expression. Antibodies to sequence 6-15 of N-end revealed only c-fos products and no FRAs were detected. Therefore FRAs have homology with the c-fos product in the C-terminal region and differ from it in the N-terminal region.

Animals↗
PubMedDate not supplied

[Isolation and characteristics of antibodies against synthetic fragments of oncoproteins. I. Antiserum against the transforming protein p28sis of simian sarcoma virus].

To generate the antibodies to the transforming protein (p28sis) of simian sarcoma virus (SSV), the rabbits were immunized with peptides, corresponding to 200-206 and 201-210 sequences of p28sis, conjugated with protein carriers by different ways. The synthesis of peptides was carried out by the classical techniques in solution by using the benzyl type side protecting groups. Antibody titres against peptides were determined by ELISA and protein specificity by radioimmunoprecipitation and immunoblotting. It was shown that the antibodies to 201-210 peptide recognize p28sis and its dimer p56sis in marmoset and rat cells transformed by simian sarcoma virus.

Animals↗