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V V Dolgov

Publications and source records attributed to V V Dolgov.

14 recordsLinked to original sources
PubMed1989

[Effect of medicinal leech preparations on indicators of atherogenesis].

Medicinal leech salivary gland secretion, administered intravenously to rats on an atherogenic diet, reduces fibrinogen level and plasma heparin tolerance and diminishes lipid deposits in the intima of large vessels. Long-term chronic oral administration of dried medicinal leech powder to rats, kept on an atherogenic diet, reduces the area of local edema in the intima of large vessels, although blood lipid composition, fibrinogen level and plasma heparin tolerance remain basically unchanged, as compared to respective values in the control animals. The salivary gland secretion reduces the 3H-thymidine incorporation into the human atherosclerotic intimal cell culture.

Administration, Oral↗
PubMed1988

Comparison of different lasers in terms of thrombogenicity of the laser-treated vascular wall.

The thrombogenic properties of the laser-treated vascular wall are reported as quantitatively assessed on the basis of 33 experiments with peripheral canine vessel segments. Three types of surfaces, namely, 1) intact, 2) mechanically de-endothelized, and 3) postlaser, have been simultaneously exposed to platelet-enriched plasma in a sequentially organized artificial circulation system. Then the adherent platelets have been counted on the treated surfaces, and the relative thrombogenicity index has been calculated according to the equation T = (A1 - Aint)/(Ad - Aint), where Aint, Ad, A1 = the adherent platelet counts on intact, mechanically de-endothelized, and laser-treated surfaces, respectively. The following lasers have been evaluated: 1) Nd-YAG, 1,060 nm, continuous wave, 4W; 2) argon-ion, 480 and 514 nm and argon-ion, 350 nm, continuous-wave, 1 W and 400 mW [corrected], respectively; 3) excimer XeCl, 308 nm, pulsed, 30 mJ per pulse, repetition rate 10 Hz [corrected]; and also 4) the laser-heated metal probe (2 mm diameter, Trimedyne, Nd-YAG) 1,060 nm, 8 W. The thrombogenicity index values obtained were 83 +/- 7, 72 +/- 8, 57 +/- 9, 63 +/- 7, and 82 +/- 9%, respectively. The differences between these values were statistically insignificant. The data are suggestive of the essential requirement of, at least, anticoagulant therapy after laser angioplasty irrespective of the laser type.

Animals↗
PubMed1988

[Thrombogenic properties of the inner vascular surface following laser exposure].

Laser angioplasty, using YAG-neodymium laser and a balloon catheter with monofiber quartz light guide (400 micron), introduced into its central canal, was performed in 8 dogs with thromboses of 11 femoral or carotid arteries. Average energy expenditure for recanalization was 26 +/- 4 J, with 4.9 +/- 0.3 W and exposure time of 5.1 +/- 0.7 s. Re-thrombosis developed after 5 to 10 days in all arteries, left in the circulation network after laser angioplasty. The latter part of the study is dealing with the assessment of the surface of femoral or carotid arteries of the dogs after laser exposure. This was done by quantitation of the adhered platelets after platelet-enriched plasma was circulated through vessels, whose inner surface was 1) intact, 2) de-endothelized, or 3) laser-damaged, using a scanning electron microscope. The thrombogenic index was 65-96, 85 and 66%, respectively, for the YAG-neodymium, argon and eximeric (XeCl) lasers.

Animals↗
PubMed1987

[Comparison of the thrombogenic properties of vascular wall after exposure to different lasers].

Quantitative thrombogenic assessment of the inner arterial surface after laser exposure was made in 33 experiments with peripheral arterial strips of dogs. In one experiment, vascular strips with native, mechanically de-endotheliazed or laser-damaged surface were consecutively placed in a platelet-enriched plasma recirculation system, after which platelet adhesion was estimated on vascular surface of the three types, using a scanning electron microscope, and the relative thrombogenic index was calculated. The laser models used included: 1) the AIG-neodymium model, 1.06 micron; 2) the argon model with visible (488 and 514 nm) and ultraviolet (350 nm) spectra; 3) the XeCl excimer type, 308 nm, as well as a preheated metal rod, imitating a ceramic or metal tip of the light guide outlet. The thrombogenic index was 83 +/- 7; 72 +/- 8; 57 +/- 9; 63 +/- 7 and 82 +/- 9%, respectively, for the above types of exposure, the differences being virtually insignificant. It is suggested that, irrespective of the type of laser employed, laser angioplasty should at least be accompanied with intensive anticoagulant therapy.

Animals↗
PubMed1986

Carrier-directed targeting of liposomes and erythrocytes to denuded areas of vessel wall.

Immunomorphological staining of specimens prepared from human carotid arteries with anti-collagen type I antibodies reveals large amounts of type I collagen in the subendothelium of lipid fibrous plaques. Collagen type I-containing structures, once in direct contact with blood after plaque rupture, can serve as potential targets for selective delivery of liposomes and erythrocytes to these areas. To verify this rationale, [14C]cholesterol oleate-containing liposomes were conjugated with bovine or human anti-collagen type I antibodies or human plasma fibronectin. Biotin derivatives of human anti-collagen type I antibody were coupled to human erythrocytes. Modified liposomes and erythrocytes were perfused in situ through segments of bovine, rabbit, or human arteries partially denuded with a balloon catheter prior to perfusion. After perfusion, the control and denuded areas were excised and subjected to scanning electron microscopic analysis and measurements of associated radioactivity. It was found that conjugates of liposomes or erythrocytes with anti-collagen type I antibodies or fibronectin are selectively bound by endothelium-free zones of arterial segments. Carrier-directed targeting of drug-laden liposomes and erythrocytes to thrombosis-prone areas of arterial lumen is discussed.

Animals↗
PubMed1985

Catecholamine-mediated injury to endothelium in rabbit perfused aorta: a quantitative analysis by scanning electron microscopy.

Perfusion of aortic segments of intact narcotized rabbits by medium 199, containing 10(-6)-10(-4) M adrenalin or noradrenalin, induced morphological injuries to endothelium: local oedema and cell losses, emergence of argyrophilic cells or cells with the widen argyrophilic cell borders. Regional changes of the mean cell density in situ were found by the quantitative scanning electron microscopy. The data obtained supports the possibility of direct injury to endothelium of aorta and large arteries as a result of intravascular infusion of catecholamines or their local therapeutic application.

Animals↗
PubMed1977

[Study of the mechanisms of adrenaline effect on the coupling of respiration with phosphorylation and K+ content in the cardiac mitochondria].

The binding of Ca2+ with fatty acids during the isolation of mitochondria from a conine heart was shown not to prevent the dissociation of respiration and physphorylation produced by adrenalin. The proteinkinase activated by cyclic AMP participates in the dissociation of respiration and phosphorylation in the mitochondria when adrenalin is administered in vivo. The reduced K+ content in the mitochondria under the effect of adrenalin is attributed to a reduction of the membrane potential of the mitochondria, the passive permeability of the mitochondria for K+ ions remaining unchanged.

Animals↗
PubMed1975

[Role of adrenaline in the changes of ion and energy metabolism in the cardiac mitochondria in experimental myocardial infarct].

The changes in the content of K+ ions, in the respiration and oxidative phosphorylation were studied in the mytochondria of canine hearts 5 and 30 min following the ligation of the left descending coronary artery. The gradual changes in the content of intramytochondrial K+ in the mytochondria of the ischaemic zone is attributed to the decreasing rate of phosphorylating respiration and dissociation of respiration and phosphorylation. Similar, but less distinct changes were noted in the mytochondria of the non-ischaemic zone. In cases resulting in ventricular fibrillation a higher dispersion of the metabolic indices was noted: decrease in K+ content, respiration and oxidative phosphorylation in the mytochondria of the ischaemic zone is nore distinct, while in the mytochondria of the non-ischaemic zone it did not differ from what is observed in a favourable course of an experimentally induced myocardial infarction. Propranolol, given in a dose of 1 mg/kg, under normal conditions decreased the respiration rate in the mytochondria and at the same time reduced the activity of succinatecytochromium-C-oxydoreductase, producing no effect on the conjugation of respiration and phosphorylation. In case of myocardial infarction Propranolol prevented the decrease of K+ and dissociation of respiration and phosphorylation. The changes in the ion and energy exchange in the cardiac mytochondria during the acute phase of myocardial infarction are attributed to the activation of the sympathoadrenal system.

Animals↗