[Outer retinal rod segment cyclic nucleotide phosphodiesterase; protein inhibitor separation, bimodal effect of GTP].
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Biomedical subjects
Publications and source records attributed to V V Furaev.
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A new set of am and ts-mutants of the phage phi80 was isolated. A series of lambdacI857h80att80int3 prophage deletion strains, extending for various lengths into the region of structural genes was generated and characterized in order to facilitate the mapping of the phi80 am and ts-mutants. Complementation test allowed to find out 5 new genes named 20, 21, 22, 23 and 24. The result of the deletion mapping of structural genes is as follows: 1-2-3-20-4-5-6-21-7-22-9-23-10-8-12-24-13.
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The participation of unsaturated (linoleic and arachidonic) and saturated (palmitic) fatty acids in reacylation of phosphatidylethanolamine (PE) in synaptosomes, photoreceptor membranes and erythrocytes at oxidative stress was studied. Induction of lipid peroxidation (LPO) was found to result in a significant decrease in the content of PE polyenoic fatty acids due to their oxidative destruction. It might be related to both an activation of phospholipase A2 and a decrease in PE reacylation rate. On contrary, under the same conditions an increase in incorporation of palmitic acid into PE was observed. The results of this study suggest that phospholipid deacylation-reacylation reactions comprise an important mechanism of both protection and adaptation of organisms to oxidative stress.
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