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Biomedical subjects

V V Kalashnikov

Publications and source records attributed to V V Kalashnikov.

14 recordsLinked to original sources
PubMed1979

[Quantitative immunoenzyme technic in the evaluation of specificity of the beta 1-g-globulin test in trophoblastic tumors].

The method of guantitative immunoenzymatic determination of beta 1-G-globulin (TSG) in the blood serum has been developed. The sensitivity of the method is 6 ng/ml TSG. It has been shown that elevated levels (12-100 ng/ml and higher) are usually observed in trophoblastic tumours of the uterus. The TSG ectopic synthesis is found to proceed in some tumours of the gastrointestinal tract and testicular teratoblastomas.

Animals↗
PubMed1979

[Immunochemical study of the specific antigens of human amniotic fluid].

Four specific antigens (trophoblastic beta 1-globulin, placental lactogen, alpha 1- and alpha 2-globulins of human placenta) were identified using antisera to the native amniotic fluid. Five antigens with the mobility of prealbumins, alpha 1-globulins, alpha 2-globulins and beta 2-globulins which bear no resemblance with the previously studied antigens were identified using antisera to the acid fraction of the amniotic fluid. Both the prealbumins and alpha 2-globulin were found in the blood serum of foetuses of different age and of newborn infants; these proteins were absent from the blood serum of pregnant women and donors. They received the names of embryonic prealbumine 1, embryonic prealbumine 2 and embryonic alpha 2-globulin. The protein with the mobility of alpha 1-globulins was found in the amniotic fluid of foetuses and in the blood serum of pregnant women only and received the name of amniotic alpha 1-globulin. The concentration of the antigens in question was studied in the developing foetuses and in the blood serum of pregnant women at different stages of pregnancy.

Alpha-Globulins↗
PubMed1978

Human embryonic prealbumin as a marker for tumours and fibroblasts.

Embryonic prealbumin (E.P.A.) was demonstrated by agar immunodiffusion in 122 out of 505 homogenates of various tumours and in 7 out of 20 serum samples from patients with connective-tissue tumours. Analogous antigen was found in human embryonic and adult cultured fibroblasts. This newly identified antigen is probably actively secreted by fibroblasts into the circulation of patients with tumours.

Adult↗
PubMed1976

Human pregnancy-specific beta1-globulin and its relation to chorioepithelioma.

Human serum pregnancy-specific beta1-globulin (beta1-GP) was localized in paraffin sections of placenta and chorioepithelioma of the uterus by indirect immunofluorescence. The structures containing beta1-GP but not human serum albumin were regarded as specifically associated with beta1-GP metabolism. Beta1-GP was found in trophoblastic cells of chorion and Langhans cells of chorioepithelioma. Using the immunoautoradiographic method (I131), we only found beta1-GP in sera of patients with trophoblastic tumours: in 74.3% of 35 patients with chorioepithelioma and in 80% of 25 patients with hydatidiform mole and destructive hydatidform mole. The diagnostic and prognostic significance of the immunochemical test for beta1-GP in trophoblastic tumours is discussed.

Beta-Globulins↗
PubMed1975

[Immunochemical identification of carcinoembryonic antigen in ovarian adenocarcinoma and pseudomucinous cystoma extracts].

Carcinoembryonic antigen (CEA) was found in 3 of 17 individual extracts of adenocarcinoma and in 7 or 8 individual samples of pseudomucinous cystoma of the human ovaries. Rabbits were immunized for the purpose of preparation of specific antisera against CEA by individual fractions (ammonium sulfate, sulfosalicylic acid and phosphotungstic acid) of CEA-positive adenocarcinomas of the ovary.

Adenocarcinoma↗
PubMed1975

[A comparative immunofluorescent study of carcino-embryonic antigen in pseudomucinous cystoma of the ovary and adenocarcinoma of the large intestine].

The indirect method of immunofluorescent analysis was applied to the study of the localization of the carcinoembryonic antigen (CEA) in pseudomucinous cystoma of the ovary and adenocarcinoma of the large intestine. CEA proved to be concentrated in the membranes and the basal part of the epithelial cells both in the pseudomucinous cystoma of the ovary and in the carcinoma of the large intestine.

Adenocarcinoma↗
PubMedDate not supplied

[alpha-fetoprotein biosynthesis in a human embryonic tissue culture].

Labelled amino acids from 14C-chlorella hydrolysate incorporated into alpha-fetoprotein during "in vitro" cultivation of fetal liver, gastrointestinal tract, lung, kidney and bone tissues, obtained with in 6-12 weeks of pregnancy. alpha-Fetoprotein was apparently synthesized in tissues of entodermal origin.

Autoradiography↗