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V V Kovaleva

Publications and source records attributed to V V Kovaleva.

11 recordsLinked to original sources
PubMed1989

[Diagnostic meningococcal sera and their quality].

The findings evidence that preparation of highly active specific meningococcal sera depends on the selection of production strains and reference cultures for the assessment of their conformity to technological requirements. Meningococcal cultures selection conditions may be unified if standard immune sera are developed, characterized by stable titres of antibodies to group capsule antigens. Employment of such sera will improve the quality of commercial agglutinating sera.

Immune Sera↗
PubMed1985

[Incidence of staphylococcal carriage in pregnant women].

In 1,494 pregnant women (on weeks 32-36) staphylococcal carriership was studied. One-third of the women under investigation were found to be carriers, their bacterial discharge increasing in spring and summer. The isolated strains proved to be polyresistant to antibiotics and belonged to epidemic strains. About a half of Staphylococcus aureus strains could not be identified with the use of the international set of phages.

Bacteriophage Typing↗
PubMed1979

[Immunological characteristics of the protein antigens of the family Neisseriaceae. II. The importance of an immunochemical analysis of the protein complexes for a study of taxonomy problems].

The study of antigenic interrelations in the family Neisseriaceae resulted in the isolation of 2 main immunologically separated variants of protein complexes: the first variant was characteristic of nonpathogenic and pathogenic species of the genus Neisseria as well as of 7 taxonomically undefined Neisseria species (N. lactamicus, N. cuniculi, N. ellongata, N. ovis, N. animalis, N. cinerea, N. canis) and Gemella haemolysans; the second variant was represented by the genera Branhamella and Acinetobacter. N. caviae and 3 out of 14 Neisseria strains of undefined species had no common antigens with the genera Neisseria and Branhamella. The importance of the immunotyping of protein complexes for studying the problems connected with the taxonomy of the family Neisseriaceae was considered.

Antigens, Bacterial↗
PubMed1975

[Isolation and identification of Neisseria in bronchial asthma].

A study was made of 29 strains of neisseria isolated from the mucosa of the pharynx and the bronchi of patients suffering from infectious-allergic bronchial asthma of different severity. Comparison of the strains by 22 tests (including the assessment of the morphological, biochemical and other signs) permitted to establish the species of neisseria with some probability. The species reference was determined in 23 cultures. N. perflava was isolated in 10 cases, N. subflava--in 6, N. flava--in 3 cases and N. sicca--in 4 cases, 2 strains corresponded to apigmentary N. cinerea. The species was not established in 4 cultures. There was found no connection between a definite microbial association and the species of neisseria and the severity of the disease. Pigment species of neisseria should be tested as diagnostic allergens.

Asthma↗
PubMed1975

[Use of the oblique illumination method for characterizing N. meningitidis cultures].

The authors used the method of oblique illumination for detection of the state of the population of meningococcus cultures. In studying 48 strains there were revealed three types of fluorescence of the colonies: bright orange with a transition into greenish-light blue along the lower margin (the I type), bright light blue with a narrow orange or green upper margin (the II type) and greyish-light blue colonies (the III type). The type of fluorescence was not associated with the sero-group specificity. Populations of meningococcus cultures, depending on conditions and duration of growth on the nutrient medium could consist both of the colonies with the same type of fluorescence, and also represent a combination of colonies with a different type of fluorescence. The colonies of the I and II types of fluorescence had marked group specificity, but mutation occurred during passage and the III type of fluorescence formed with a loss of serological specificity. Continuous selection of the colonies of the I and the II types of fluorescence permitted to preserve the population with specific properties necessary for production of diagnostic and vaccine preparations for a long time.

Antigens, Bacterial↗
PubMed1975

[Assessment of the condition of the population of gonococcal cultures].

Application of the method of oblique illumination permitted to reveal nonhomogeneousness of the cultures isolated from the patients with a different (acute and torpid) course of the disease. Passage of the freshly-isolated cultures on the nutrient media led to the appearance of mutants characterized by the fluorescence of the third type which also characterized all the 48 museum cultures stored for a long time on the nutrient media. The method suggested permits not only to evaluate the gonococcal cultures condition but also to carry out their directed selection.

Bacteriological Techniques↗