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Biomedical subjects

V V Makarov

Publications and source records attributed to V V Makarov.

At least 19 recordsLinked to original sources
PubMed1991

[The identification of the membrane proteins of human and animal viruses].

The review deals with the methods of identification of virus-specific proteins on virion and infected cell surface. The isotopic labeling of membrane proteins, their extraction by proteolytic enzymes and selective solubilization by detergents are considered. The plasmatic membrane isolation by centrifugation and by means of microcarriers is described. The methods of membrane protein localization using monoclonal antibodies and radioimmunoprecipitation are presented.

Animals↗
PubMed1985

Red cell enzyme types in rheumatic diseases.

We studied the frequencies of red cell enzyme types, AcP, PGM1 and EsD in 213 patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), rheumatic heart disease (RHD), scleroderma (Scl) and psoriatic arthropathy (PsA). The differences in frequency of AcP phenotypes between RA, Scl, and PsA and the Moscow population were significant. In PsA the PGM1 phenotype 1-1 frequency was significantly decreased, while the phenotype 2-1 frequency was significantly increased.

Acid Phosphatase↗
PubMedDate not supplied

[The functional role of the glycosylation of viral components].

An inhibitory analysis of the role of glycosylation and glycoproteins in the manifestations of the most important properties of African swine virus was carried out using a set of strains and variants with contrasting characteristics. Glycoproteins were shown to realize some virus functions such as virulence and its variability, intracellular transport and exocytosis of virions, hemadsorption, but not immunological recognition of the infected cells by cytotoxic T-lymphocytes.

African Swine Fever Virus↗
PubMedDate not supplied

[Polypeptides p14 and p31 of the African swine fever virus--early proteins located on the membrane of the infected cell].

African swine fever virus polypeptides p14 and p31 are synthesized in the presence of phosphonacetic acid which inhibits viral DNA replication, and therefore they are early viral proteins. These polypeptides were found to be localized on plasma membranes by immunofluorescence with monospecific antisera and monoclonal antibodies and by selective solubilization of infected cells. The p14-specific antibodies mediate complement-dependent cytolysis and antibody-dependent cytotoxicity of the cells infected with African swine fever virus.

African Swine Fever↗