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Biomedical subjects

V V Mikhaĭlov

Publications and source records attributed to V V Mikhaĭlov.

At least 19 recordsLinked to original sources
PubMed2000

[Immunoenzyme method for detecting microbial producers of palytoxin].

A competitive ELISA using the intact toxin as a coating antigen for detecting palytoxin was developed. This immunoassay allows palytoxin (PTX) to be determined in the range of 6-250 ng/ml. In sensitivity, this determination is comparable with RIA but is three times inferior to ELISA using monoclonal antibodies. Inhibition experiments using some toxins of marine invertebrates proved the serological specificity of the palytoxin binding to antibodies. Both the indirect and competitive ELISA were used to find PTX-producing bacteria among 420 isolates of sea bacteria. It was found that gram-negative bacteria Aeromonas sp. and Vibrio sp. associated with toxic samples of the soft coral Palythoa sp. produced compounds antigenically related to PTX.

Acrylamides↗
PubMed1995

[A novel fungicide of the iturin group, obtained from a marine isolate of Bacillus subtilis. Isolation, physico-chemical and biochemical properties, identification].

Strain KMM 457 of Bacillus subtilis was isolated from a frozen sample of the soft coral Sarcophyton sp. The samples were collected in 1989 in the South China Sea near the Vietnam Shore during an expedition on the board of R/V "Akademik Oparin". Metabolites of the isolate were investigated and it was found to produce a number of physiologically active compounds. One of them designated as OGA showed fungicidal activity. By the physicochemical properties it was referred to the group of iturins. The comparison with the described antibiotics of the iturin group suggested that it was a new representative of the iturin group.

Anti-Bacterial Agents↗
PubMed1995

[Bioactive compounds from marine actinomycetes].

Studies of the origin of bioactive metabolites of marine actinomycetes are reviewed. Structures and properties of new metabolites from indigenous marine bacteria from Actinomycetales order, such as a benzanthraquinone antibiotic from a strain of the Chainia purpurogena, istamycins, aplasmomycins, altemicidin, new phenazine esters. C13-butanolide, marinone and debromomarinone, palmyromycin, urauchimicins and some others compounds are presented. Prospects of marine biotechnology and microbiology (with considerable emphasis on the development of the basis biology of marine microorganisms in cultures collection) are discussed.

Actinomycetales↗
PubMed1993

[Peroral immunization--a method for enhancing the safety of the recombinant vector (the vaccinia virus)].

Oral and dermal administrations of vaccinia virus into rabbits, guinea pigs, and monkeys demonstrated a milder (without homeostasis disturbance) course of the vaccinal process with oral immunization, intensive immunity forming in minimal sensitization of the body was compared with dermal one. The results of revaccination in adults with oral smallpox vaccine and primary immunization in Ethiopia showed that oral immunization with vaccinia virus was safe, effective and lowly reactogenic. The comparative study of the preparation in remote revaccination (5 or more years later) proved its advantage over dermal vaccine. It consists in ecological safety (vaccinia virus excretion into the environment during 1 and 11 days, respectively, by 10-fold concentration reduction), reactogenicity (5 and 33% of common reactions, respectively) in the same immunogenicity.

Administration, Cutaneous↗
PubMed1993

[The mechanism of trophic effects of salivary glands on oral mucosa].

Using standard HPLC method the catecholamines' content in submaxillary salivary glandular tissue and in oral mucous membrane non-stimulated and stimulated by pilocarpine salivary glands during desalivation and saline solution washing has been studied. In stimulated salivary glandular tissue and oral mucous membrane resulted in diminishing of noradrenaline as well. It was concluded that oral mucous membrane gets noradrenaline in period of basal secretion and spends it during stimulated secretion.

Animals↗
PubMed1993

[Correlation of protein content in salivary gland tissue, oral mucosa and saliva in experimental staphylococcal sialoadenitis].

The dependence between the total content and excretion of protein (P) in salivary gland tissue (SGT), oral mucosa (OM) and saliva has been investigated. The difference between P contents in intact gland and during staphylococcus sialoadenitis has been shown. After 2 hours of staphylococcus toxin injection in non-stimulated SGT P contents are not changed, and in saliva--are increased. After 24 hours in non-stimulated SGT P contents are decreased, in OM P contents are increased. By stimulation of SGT, P contents increases, in OM--decreases.

Animals↗
PubMed1993

[The interrelationship of the protein content of the tissue of the submandibular salivary glands and the mucosa during the secretory cycle in acute inflammation of the oral soft tissues].

The Lowry method was used to estimated protein levels in the submaxillary salivary glands, oral mucosa and saliva during pilocarpine-induced and uninduced secretion in acute Staphylococcal inflammation of oral soft tissue. It has been shown that protein levels were unchanged in the salivary glands and oral mucosa in unstimulated and stimulated secretion following 2 hours. After 24 hours, they were also unchanged in the salivary glands and oral mucosa in unstimulated secretion, but in stimulated secretion the levels of protein were increased. It is concluded that in pilocarpine-induced secretion, there was increased defense of mucosal soft tissue and salivary gland tissue due to higher protein levels in the interstitial space.

Acute Disease↗
PubMed1993

[The interrelationship of the catecholamine and protein content of the tissue of the submandibular salivary glands and the mucosa during the secretory cycle in chronic inflammation of the oral soft tissues].

In rats with local chronic carrageenin-induced inflammation of oral soft tissue, the levels of catecholamines and protein were measured in the submaxillary salivary glands, oral mucosa during pilocarpine-induced and background secretions and in saliva during induced secretion. The levels of epinephrine and protein were found to be increased during background secretion in the oral mucosa in chronic inflammation. After stimulation of a secretory cycle in the salivary glands, the contents of epinephrine were increased, but those of protein were decreased. The levels of epinephrine, norepinephrine and proteins were higher in the oral mucosa. It is concluded that chronic oral soft tissue inflammation results in an increase in neurotrophic effects on oral tissues, which contributes to the formation of their protection preventing the generalization of acute inflammation and its transformation to chronic forms.

Animals↗
PubMed1993

[The interrelationship of the content of catecholamines in salivary gland tissue, oral mucosa and in the saliva in experimental staphylococcal sialoadenitis].

Catecholamine contents in non-stimulated and pilocarpine-stimulated submaxillary salivary gland tissue (SGT), oral mucosa (OM) and stimulated saliva in acute experimental staphylococcal sialoadenitis were determined in rats using standard HPLC method. It was shown that 2 hrs after toxin injection, NA contents decreased in non-stimulated SGT, NA and A contents in SGT and OM also decreased. After 2 and 24 hrs NA and A contents were not changed in stimulated SGT and OM and after 24 hrs A content increased in SGT and OM. In saliva after 2 hrs the content and secretion of NA increased, after 24 hrs the content and secretion of NA decreased, but that of A increased. It was concluded that in sialoadenitis the disorders of catecholamine contents in the saliva correlated with its content in OM and influenced the trophic of OM and could be conductive to the development of its pathology.

Acute Disease↗
PubMed1993

[Catecholamines in salivary gland and oral mucosal tissue and in the saliva in acute inflammation of the oral soft tissues].

Catecholamine contents in non-stimulated and pilocarpine-stimulated submaxillary salivary gland tissue (SGT), oral mucosa (OM) and stimulated saliva in acute staphylococcal inflammation of soft oral cavity tissue were determined in rats using the standard HPLC method. It was shown that 2 hrs after toxin injection NA contents decreased in non-stimulated SGT. After 24 hrs A contents decreased too as well as HA contents in OM. After 2 and 24 hrs HA contents increased in stimulated SGT, in OM HA contents did not change but A contents after 24 hrs decreased. In saliva, after 24 hrs these levers were normalized. The authors concluded that the inflammatory process in the soft oral cavity tissue was the cause of basal and stimulated salivary secretion disturbance.

Acute Disease↗