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Biomedical subjects

V V Morozov

Publications and source records attributed to V V Morozov.

At least 19 recordsLinked to original sources
PubMed1980

[Effect of genetic and environmental factors on the variability of the lipid and lipoprotein concentration in the blood plasma].

Family and twin data (45 parent -- children and 35 MZ and 55 DZ pairs) with average age of 37 years were analysed. The type of zygocity was determined by phenotyping erythrocyte enzymes and serum antigens and by calculating discriminative functions with the use of antropometric, antroposcopic, and dermatoglyphic measurements. The distribution component of phenotypic dispersion was made from correlation between relatives according to the Trubnikov - Gindilis - Finogenova scheme. The following distribution was obtained: with CH the genetic additive Ga = 82%, the genetic dominant Gd = 0%, and the environmental commom Ec = 0%, environmental within Ew = 18%; with TG, Ga = 46%, Gd = 2%, Ec = 21%, and Ew = 31%; with CH-HDL, Ga = 50%, Gd = 0%, Ec = 16%, and Ew = 34%. The importance of genetic and environmental components in the determination of CH, TG, CH-HDL, CH-LDL in plasma is discussed.

Adult↗
PubMed1980

[Cholesterol of the high-density lipoprotein subclasses in the native inhabitants of the Chukchi National Autonomous Okrug].

The authors studied the content of total cholesterol (Ch), triglycerides (TG), CS of high density lipoprotein (HDL2 and HDL3) subclasses and testosterone in blood plasma of 30-59-year-old males, natives or newcomers of Chukotsk, and compared the results with the corresponding values determined in the male population of Moscow. It was established that the mean HDL Ch concentration in blood plasma was higher and the content of TG and to a lesser degree that of total CS, was lower in the Chukchi males than in the male Moscow population and in the newcomers who were examined. It was also shown that in hypo- and hyper-alphalipoproteinemia in all groups examined, the content of HDL2 Ch changed for the most part (decreased or increased, respectively) while the level of HDL3 Ch remained relatively stable.

Adult↗
PubMed1975

[Use of an ultramicroelectrophoretic method for separating blood serum cellular and isoenzymes].

Methods for staining of ultramicroenzymelectrophoregrams are described. Sharp and well reproducible bands of isozymes of LDH, MDH, G-6-PD, AcP, AlP, alpha-NAE were obtained from homogenates of cultivated human fibroblasts, of rat's liver, kidney and brain, of human embryonic liver, and in blood serum of man and frog. An extract from the rat brain containing only 3.4 ng of proteins or a quantity of 70-80 fibroblasts were sufficient for obtaining a well differentiated spectrum of LDH isozymes. The ultramicromethod significantly lowers the need in deficit reagents, reduces the cost and time of analysis.

Acid Phosphatase↗
PubMed1975

[Modified method of ultramicroelectrophoretic protein fractionation].

A modification is described for the method of ultramicroelectrophoretic fractioning of proteins in polyacrylamide gel in capillaries 400--100 microns in diameter. The original construction of the assembly of apparatuses is offered which can be assembled from details made in the USSR. The operation of dosed filling of capillaries is significantly facilitated. The optimal quantity of protein in one sample reaches 1.5--0.1 mkg (depending on the capillary diameter). The reproducability and sharpness of protein fractions by the offered method are not worse compared with the generally accepted method of macro-disc electrophoresis.

Cell Fractionation↗
PubMed1975

[Simple method of selection of a sample volume in ultramicroelectrophoresis of proteins].

The tentative optimal values of the protein sample quantities were determined for the ultramicroelectrophoretic fractionation in the polyacrylamide gels in the capillaries of different diameters which provided distinct and highly reproducible electrophoregramms. A simple calculation (by means of table and formula) of the volumes of protein solutions required for different concentrations and different diameters of capillaries is suggested.

Electrophoresis, Polyacrylamide Gel↗