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Biomedical subjects

V V Nadtochiĭ

Publications and source records attributed to V V Nadtochiĭ.

15 recordsLinked to original sources
PubMed1984

[Transport of 125I-poly I : poly C incorporated in liposomes from the enteric cavity to the small intestine mucosa. An electron microscopic autoradiographic study].

Transport of 125I-poly(I) : poly(C) incorporated into liposomes trough the small intestine mucose was investigated by electron microscopic autoradiography. With the migration of liposomes into the mucous layer on the luminal surface of the intestine up to the glycocalix level of microvilli these undergo degradation with the formation of monolayer liposomes from which polynucleotide is released. Later on the poly(I) : poly(C) or its fragments transported through the enterocytes to be accumulated in cells of the connective tissue stroma of the small intestine mucose. Part of polynucleotide was incorporated up to the arterial and lymphoid capillary level. Apparently, on the way of its transport the polynucleotide is affected by pancreatic and tissue nucleases. The accumulation of polynucleotide in macrophages, fibroblasts, lymphocytes, plasma cells and smooth muscle cells was traced. It is supposed that the polynucleotide accumulated in stroma of the small intestine mucose may preserve its interferon inducing activity.

Animals↗
PubMed1984

[Liposome transport as a model of substance absorption in the small intestine studied by electron microscopic autoradiography and scanning microscopy].

Using electron microscope autoradiography and scanning microscopy, evidence was provided on the space-time character of the transport of enterally introduced liposomes containing 125I-lecitin and 3H-cholesterine. Liposomes underwent degradation on the mucous surface of the epithelial cells, followed by the appearance of monolayer vesicles to be transported to the glycocalix area between the microvilli. This is accompanied with the release of the radioactive trait from liposomes and its transporting to enterocytes.

Animals↗
PubMed1983

[Electron microscopic study of 3H-leucine transport between the internal body environment and the enteral environment].

Using electron microscopic autoradiography, a space-time characteristics of 3H-leucine transport, from the enteral to the inner environment, and from blood plasma to the enteral environment, has been given. Some gradient of intracellular leucin accumulation is determined, from the tip cells of a villus to epithelocytes of the basal membrane. Amino acid transport from blood plasma to the lumen of the intestine is accomplished mainly via the cytoplasm and intercellular space of epithelocytes of the villus basement. It is proposed that amino acid transport through membranes of microvilli and through basal-lateral membranes of cells being on various levels along the villus may start at different amino acid concentrations which may be, presumably, to different concentration and different conjugation of enzyme-transport complexes in these membranes.

Animals↗
PubMed1983

[Absorption routes of stearic acid and leucine in the small intestine of the rat after gamma irradiation].

The aim of the investigation was to study transport of 3H-stearic acid in ultrastructural compartments of epitheliocytes during absorbtion, resynthesis and translocation of lipids having the form of chylomicrons after a local large fractionated gamma-irradiation of the rat small intestine with a total dose of 35 Gr. In the anesthesized animals the ligated fragments of the jejunum, which kept their blood supply and innervation, were separated, fatty emulsion with 3H-stearic acid (a label) was administered into their lumen. Intensity of the label incorporation was quantitatively estimated in the electron microscopic autographs every 2, 5, 10 and 20 min after the isotope administration. Time distribution of the label concentration after irradiation characterized the sequence of the transport stages of 3H-stearic acid across microvilli and the terminal reticulum (MV + TR) triglycerides resynthesis in the agranular and granular endoplasmic reticulum (AER, GER), chylomicrons formation in Golgi complex (GC), as well as their exudation into the lacunar intercellular space (LIS), basal membrane and capillaries. Transport deceleration of the label at the level MV + TR and decreasing synthesis of triglycerides in GER was accompanied with a delay in the chylomicrons formation in the GC and their excretion into the LIS. Conglomerates of the aggregated chylomicrons were formed in the LIS. The radioautographic data on 3H-leucine incorporation characterized a decreased activity of the protein-synthesizing system of epitheliocytes.

Animals↗
PubMed1982

[Absorption study of 3H-stearic acid in the rat small intestine using electron microscopic autoradiography].

Using electron microscope autoradiography evidence was provided on the space time character of the absorption, resynthesis, transfer and exocytosis of lipids in the form of chylomicrons; this evidence correlating with data on distribution in the subcellular compartments of epithelial cells of the intestine. The majority of fatty acid enters the lymph in the form of resynthesized lipids. Part of fatty acid is transported from the enteral environment to the internal one, escaping from the stages of esterification and triglyceride and chylomicron formation in the endoplasmic reticulum and Golgi apparatus.

Animals↗
PubMed1977

[Monoamine oxidase activity and ultrastructure of subcellular fractions of the cerebral cortex under normal conditions and in the presence of phenamine stimulation].

Crude mitochondrial fractions were fractionated into purified mitochondria, heavy- and light synaptosomes and synaptosomal membranes. The highest MAO activity was observed in purified mitochondria and heavy synaptosomes. Amphetamine strongly inhibited the MAO activity in heavy synaptosomes, and only slightly affected light synyptosomes. Electron microscopy of heavy synaptosomes revealed specific vesicles suggesting the presence of nerve endings of monoaminergic neurons, and the increase of the functional activity of these neurons.

Amphetamine↗
PubMed1976

[Method of ultrastructural analysis with the aid of measuring television complex].

The area of the section of presynaptic dense projections of the active zone of axondendritic synapses of the rat's cortex was measured using a television complex. Tissue specimens were contrasted with phosphotungstic acid. Morphometry was carried out in the regime of error correction in measurement, the errors arising in scanning process of image. The area of the section of mitochondria, lysosome, nucleoli, channels and tanks of the endoplasmic reticulum may be measured by the method suggested. Quantitative data, obtained by the morphometry of the synapse ultrastructure, may serve a basis for understanding the mechanism by which synapses are involved in information processes of the central nervous system.

Animals↗
PubMed1976

[Neurospecific protein S-100 in the synaptosomes of rat cerebral cortex].

Neurospecific S-100 protein was revealed by the methods of microelectrophoresis in the 15% polyacrylamide gel with a 0.1% sodium dodecylsulfate and by a highly purified S-100 protein "marker" in the composition of low molecular acidic proteins of the rat brain synaptosomes. The S-100 protein conten constitutes about 15-2o% of the low molecular acidic synaptosomal porteins in the rat brain.

Animals↗
PubMed1976

[Incorporation of tritiated amino acids into the ultrastructure of the cerebral cortex following phenamine stimulation].

The method of electronoscopic autoradiography has revealed an increased biosynthesis of protein by neuronal and glial cells of the motor cortex of rats under phenamine stimulation. The increased level of incorporation of labelled precursors in the molecular layer of the cortex is likely to be associated with increased accumulation of newly synthetized protein in axo-dendritic synapses due to stimulation of the rapid component of the axonal transport of macromolecules. At the same time, participation of the autonomic protein-synthetizing system in the biosynthesis of synaptic proteins is not excluded. The labelled products were found to localize in the field of the thorn apparatus in processes of the "structural-functional" adaptation of synaptic entrances (A. A. Manina, 1972).

Amphetamine↗
PubMedDate not supplied

[Electron-autoradiographic and biochemical study of the role of foam cells in low density lipoprotein metabolism].

Participation of foam cells in metabolism of lipoproteins of low density (LPLD) was studied by biochemical methods and by electronic autoradiography. The foam cells were isolated from atherosclerotic rabbit aorta after perfusion with 12I-LPLD within 6 hrs. Metabolic activity of foam cells was evaluated by the ratio of radioactivity in lipid and protein components of cells as well as by distribution of reduced argentum granules among the subcellular structures on autoradiogram. The biochemical studies showed that the lipid components of LPLD were preferably accumulated in foam cells. Electronic-autoradiography demonstrated that protein from the lipoprotein particles was also distributed intracellularly in various cytoplasmic structures. Foam cells appear to capture intact lipoprotein particles by pinocytosis with subsequent splitting of protein component by cellular lysosome apparatus.

Animals↗