Biomedical subjects
Publications and source records attributed to V V Neustroeva.
Examining the interaction of M. hominis, M. fermentans, and M. arthritidis with peritoneal macrophages of various strain mice has demonstrated that the interaction is related both to the mycoplasma and macrophage types. It has been found that M. arthritidis may multiply and persist for a long time in the macrophages, which is followed by morphological changes developed in the latter and by suppression of phagocytic activity.
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Experiments in CHO cell cultures have demonstrated the cytotoxic action of culture filtrates obtained after growing L. pneumophilia strain Philadelphia I, serogroup 1, in a liquid culture medium. This cytotoxic activity has been found to differ in its character from that described in earlier works. The dynamics of the accumulation of cytotoxin in the culture medium has been studied, and the work aimed at the determination of the limits of some cultivation parameters, within which the maximum toxin formation is observed, has been carried out.
Cytogenetic analysis of mouse hybridoma producing monoclonal antibodies to diphtheria toxin and of its derivative, that lost secretory activity at the third passage in vivo, has been carried out. 58% cells of antibody secreting cell lines belonged to a modal class (76-79 chromosomes per cll). The modal chromosomal number of the subline that has stopped producing antibodies decreased to 63-66 per cell and the stem line of this derivative consisted of 30% of cell population only. Chromosome aberrations were much more frequent in hybridoma cells, that ceased to secrete antibodies, than in cells of original hybridoma: 32.3% of aberrant metaphases (1.38 break per cell) and 6.3% of aberrant metaphases (0.1 break per cell), respectively. Mycoplasma infection was found in the hybridoma subline that stopped producing antibodies as defined by the microbiological and cytochemical techniques. Mice might be the possible source of infection. By means of cloning of hybridoma variant, that did not secrete immunoglobulins, several sublines with the recovered secretory function were obtained.
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The survival of the L-forms of group A streptococci in peritoneal macrophages of SHR mice has been studied microbiologically and by the method of indirect immunofluorescence in vitro and in vivo. The phagocytosis of the L-forms, proceeding more actively in vivo, has been found to occur within 1-3 days after inoculation. The preservation of some viable L-forms in macrophages for 6-7 days may apparently facilitate their persistence in the body.
Specimens of synovial fluid taken from patients with rheumatoid arthritis were tested for the presence of mycoplasmas and mycoplasmic antigens. In 30% of cases the direct inoculation into cell-free media permitted the detection of mycoplasma-like agents which could not be subcultured on solid media for identification. Mycoplasmic antigens were detected in the tested material with the same frequency by means of the immunofluorescence test. The use of cell cultures made it possible to isolate and identify mycoplasmas. M. arthritidis and M. fermentans, as well as their association, were identified in the immunofluorescence test and in cell cultures.
Interrelations between the L-forms of group A beta-hemolytic streptococci and mouse peritoneal macrophages have been studied by electron microscopy. The macrophages have been shown to actively phagocytize L-form cells in great amounts. Most of the phagocytized L-forms are destroyed and become nonviable, but a few of them survive as elementary bodies within 48 hours.
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Using vital stain chlorazol-black E the authors studied the localization and behaviour of 5 mycoplasma species and the stable L-form of beta-hemolytic streptococcus of group A in various continuous cell lines. Mycoplasmas and L-forms had a definite evolution cycle in the cells. At first they are arranged extracellularly and on the surface of the cells, and then intracellularly where they multiply intensively; later they are again localized on the surface of the cells and extracellularly. This cycle proved to depend on the type of infection. The character of the localization depended on the type of the causative agent and of the culture.
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A study was made of sorption of 6 continuous lines of cells on the colonies of 55 M. hominis strains isolated from patients with inflammatory diseases of the urogenital tract. M. hominis proved to be capable of sorbing various cell cultures on the colonies. The sorption depended on the type of the culture. The greatest sorption capacity was possessed by the cells of human origin --FL and HeLa. M. hominis strains under study differed by their capacity to cell sorbtion. The method of cell sorption on the colonies can be suggested for the determination of heterogeneity of the population and of the intrastrain differences in M. hominis.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.