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V V Nikiforov

Publications and source records attributed to V V Nikiforov.

At least 19 recordsLinked to original sources
PubMed1997

Site-specific endonuclease AbaI from Azospirillum brasilense UQ 1796 is an isoschizomer of endonuclease BclI.

The site-specific endonuclease AbaI was isolated and purified to functional purity from the soil nitrogen-fixing bacterium Azospirillum brasilense UQ 1796. Purification included successive chromatography on columns with phosphocellulose, heparin-Sepharose, and hydroxyapatite. The purified enzyme recognizes the palindromic DNA sequence 5'-T decreases ATCA-3' and cleaves it as shown by the arrow. The isolated enzyme belongs to class II restriction endonuclease and is an isoschizomer of endonuclease BclI. The enzyme of AbaI is active at 26-56 degrees C. The optimal temperature is 48 degrees C and the optimal buffer is LRB.

Azospirillum brasilense↗
PubMed1982

[Dengue fever].

Explore the source record for details and available documents.

Adult↗
PubMed1981

[Isolation of DNA-membrane complexes from Bacillus subtilis].

The DNA-membrane complexes were isolated from the membranes of Bacillus subtilis, disrupted by ultrasonication and subjected to chromatography on Sepharose 4B. The membrane-bound fraction of native 3H-DNA was found only in the freshly isolated material. The phospholipid and protein composition of this fraction was determined. It was assumed that the newly synthesized membrane-associated 3H-DNA from Bacillus subtilis is bound to the lipoprotein complexes enriched with phospholipids.

Bacillus subtilis↗