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V V Shikhodyrov

Publications and source records attributed to V V Shikhodyrov.

At least 19 recordsLinked to original sources
PubMed1986

[Automatic image analysis in the study of the microcirculatory bed].

The authors discuss the possibility of efficient morphometric analysis of capillary architectonics and pathological structural changes in microcirculatory bed with computer systems of image analysis using selective histological staining of microvessels. It is suggested that a histochemical method of alkaline phosphatase identification is used for cerebral, cardiac, adrenal, hepatic, splenal, gastrointestinal, sceletal muscle, testes, urine bladder, serosae and other rat organ capillaries computerized analysis. It is recommended to impregnate the microcirculatory bed of bigger omentum of large laboratory animals according to V. Kupriyanov's method, and to stain the labrocytes of the perivascular loose connective tissue of serious membranes in large laboratory animals with alcohol solution of toluidine blue.

Animals↗
PubMed1986

[Multivariate statistical analysis of tissue basophils in acute radiation sickness].

By means of mark scale estimation method, discriminant and factor analyses, changes in integral indices on the state of mast cells in the rat mesentery have been investigated in dynamics of medullary, intestinal and cerebral forms of an acute radiation sickness. The integral indices are calculated basing on the morphometric parameters of the cells, that are obtained after the histological preparations are treated in a special automatic system for analysing images. At the medullary form of the acute radiation sickness, the greatest structural rearrangements of the mast cells take place during the first hours after the radiation, as well as during the climax of the disease, at the intestinal form, the analogous changes are revealed 1-3 days after the effect, and at the cerebral form--3 h after the radiation. The integral indices, calculated by means of the three methods, are well correlated with each other.

Acute Disease↗
PubMed1985

[Pathology of the rat mesenteric lymphatic bed in acute radiation sickness].

The authors have shown that in the medullary, intestinal and cerebral forms of acute radiation disease pathological changes of the lymphatic microvessels manifested themselves in the form of lymphostasis at the climax of disease in the medullary form of acute radiation disease and at the climax and in the terminal period in the intestinal form. A characteristic feature of the lymphatic channel involvement in the medullary form of acute radiation disease was a large number of erythrocytes in the lumen of the lymphatic vessels at the climax of disease. No changes of the mesenteric lymphatic channel of the rats were noted in the cerebral form. On the other hand, the presence of a large number of newly formed capillaries in the young rats at the climax of disease indicate the persistence of the proliferative ability of the lymphatic microvessel endothelium and its significant radioresistance.

Acute Disease↗
PubMed1984

[Changes in the microcirculatory bed of the mesentery in the rat in the intestinal form of acute radiation disease (experimental morphologic study)].

Total mesentery preparations of the rat after single total irradiation at the dose levels of 15 and 30 Gy were examined by V. V. Kupriyanov 's method and staining by N.E. Yarigin and S.V. Panchenko . Monotonous pathomorphological changes characteristic of microcirculation disorders were revealed such as blood overdistribution into the venous part of microvasculature prestasis , stasis and lymphostasis. Apart from these qualitative alterations, morphometry of certain parts of microvasculature was performed and some indexes and ratios were calculated. Changes of quantitative parameters were as follows: increase of the capillaries, postcapillaries and venules diameter, increase of their surface and volume, increase of diameter of pericapillary diffusion zone; decrease of the correlation ratio between total arteriolar influx and total venular blood passage within a mesentery segment; decrease of the calculated linear speed of blood in capillaries, postcapillaries and venulas .

Animals↗
PubMedDate not supplied

[Morphological changes in lungs during chest irradiation].

Morphological characteristics of the lung are presented for rats and hamsters with esophageal and lung damages following chest exposure to 10-100 Gy. A four-month post-exposure follow-up revealed changes in the lung tissue in all cases. Depending on radiation dose, lung damages differed in genesis and time of development. These were either extensive secondary suppurative inflammation in the bronchi and alveolar parenchyma, to the point of empyemas, or radiation injuries proper of alveolar and bronchial epithelia, being degenerative and atrophic in nature. In four months after exposure to 18-42 Gy, the hamsters exhibited a pathological process, i.e. a basis for pulmonary fibrosis and cardiopulmonary insufficiency to be developed.

Animals↗