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V V Sirotkin

Publications and source records attributed to V V Sirotkin.

8 recordsLinked to original sources
PubMed1993

[Dual-factor correlation analysis of the distribution of morphofunctional properties of mitochondria].

Judging by double-factorial correlative analysis of mitochondrial morpho-functional property distribution during the treatment of organism with radioactive rays, it has been established that there is a high correlation between mitochondrial dimensions in population and known structural-functional types. To detect the force and tightness of this parameters' connection, there lists a method of double-factorial correlative analysis. The value of correlation coefficient lets assume that mitochondrial functional activity depends on mitochondrial dimensions and is described by straight regression equation. It has been established that dimension's increase is accompanied by mitochondrial functional activity decrease and vice versa, though some functionally inactivated mitochondria can have little dimensions. Thus, knowing mitochondrial dimensions in population we can detect it's functional properties and detect mitochondrial dimensions knowing the functional properties by straight regression curves.

Animals↗
PubMed1993

[Three-factor correlation analysis of morphofunctional changes in mitochondria based on the results of morphologic and biochemical studies].

The multifactorial correlative analysis showed the tightness and correlative connection between the time after one time irradiation of experimental animals, breathing and redistribution of hepatocytes' mitochondria dimensions. It has been established that the velocity of mitochondrial breathing can be described by linear correlative dependences from time and area, negative coefficients in equations shows that the velocity of breathing decrease when time and mitochondrial area increase. This equations let to estimate mitochondrial population state, depending on time and their dimensions and show that big mitochondria perish in a more brief time. It was assumed that the velocity of destruction is depend on irradiation dose and it leads to the change of equation coefficients.

Animals↗
PubMed1988

[Effect of irradiation on the size of the mitochondria in rat cardiomyocytes].

The paper presents the results of studies of the influence of radiation on the sizes of cardiomyocyte mitochondria in rats. Considering the established regularity of subordination of mitochondrial size distribution to the gamma-law on the cardiomyocyte mitochondria, one can judge presumably on their functional state by the variation in the mitochondrial size distribution. This conclusion is confirmed by the relationship between the redistribution of mitochondrial size and the biochemical parameters of their functional activities. It is recommended to evaluate morphometrically the variation in mitochondrial sizes in the course of computer-assisted microscopic investigation of cells for the purpose of assessment of morphological and functional properties of these cellular organellae and the cell as a whole.

Animals↗
PubMed1986

[Effect of irradiation on the size of isolated liver mitochondria].

The role of external effects in the time course of changes in hepatocyte mitochondria size was studied. Suspensions of mitochondria isolated from the liver of inbred mice were investigated at various periods after gamma irradiation. Statistical analysis of changes in the size of isolated mitochondria has shown that irradiation leads to an increase in mitochondria size, reflected in the distribution pattern. The changes may be related to the functional activity, and distribution shifts may be regarded as manifestations of hepatocyte adaptation.

Animals↗
PubMed1981

[Morphofunctional characteristics of mouse liver mitochondria (morphologic and biochemical study)].

A study was made of a correlation between the size of intact mitochondria and biochemical parameters of their activity. A suspension prepared from mitochondria isolated from the linear mouse liver at varying times after gamma-irradiation was on object of investigation. Electron microscopy was performed along with a polarographic examination of mitochondrial respiration and oxidative phosphorylation. It was shown that the negative contrast examination of mitochondria followed by quantitative analysis of their appearances on the electron microscopic photographs enables making an accurate enough and objective judgement of mitochondrial functional activity.

Animals↗