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Biomedical subjects

V V Vardhani

Publications and source records attributed to V V Vardhani.

At least 19 recordsLinked to original sources
PubMed2005

Liver phosphatases in mice treated with lead during murine ancylostomiasis.

Both infective larvae and pollutants disturb the homeostasis of female Swiss albino mice, causing ancylostomiasis and severe immunophysiological changes. In our experimental design, mice in group A were fed 0.01 mg of lead nitrate before infection with 500 larvae of Ancylostoma caninum, mice in group B were infected with larvae without previous lead treatment, and group C received lead nitrate treatment alone. Control animals in group D were neither treated with lead nitrate nor infected with larvae. While all three experimental groups showed disturbances in liver alkaline phosphatase (ALP) and acid phosphatase (ACP), group C receiving lead nitrate treatment alone and group A receiving lead nitrate with infection showed increased levels of ACP and decreased levels of ALP.

Acid Phosphatase↗
PubMed2003

Eosinophil relationship in gut anaphylaxis during experimental ancylostomosis.

Changes induced in the circulating and intestinal eosinophil levels and worm load were studied in female Swiss albino mice infected with multiple doses of Ancylostoma caninum larvae. There was a significant rise of both circulating and intestinal eosinophils in all the infected animals when compared with controls. In all the experimental groups of animals, peak eosinophilia was observed during the third week of post-infection and sustained upto 30th day with slight alterations. These studies indicated that eosinophilia will be induced due to anaphylactic reactions in the gut and eosinophilia in multiple infections is an anamnestic response.

Anaphylaxis↗
PubMed2002

Effect of lead and ancylostomiasis on phosphatases of heart in Swiss albino mice.

Administration of 0.01 and 0.1 mg of lead nitrate for 4 and 7 days and infection of Ancylostoma caninum larvae orally altered the activation of alkaline phosphatase and acid phosphatase in the hearts of mice when compared to infected animals and controls. Alkaline phosphatase activity increased significantly in all drug-treated + infected mice. The level of acid phosphatase decreased significantly in mice exposed to chronic doses of lead. The altered levels of alkaline phosphatase and acid phosphatase suggest that administration of lead could cause toxicity in the heart, disturbing the cellular metabolism; infection alone could not cause any significant changes in enzymes of heart.

Acid Phosphatase↗
PubMed1992

Eosinophilia in murine ancylostomiasis.

Eosinophil changes in the peripheral blood of singly and repeated infected mice were studied during murine ancylostomiasis. In singly infected animals, eosinophils began to rise on 1st day, reached a peak on 4th day and progressively declined from 9th to 30th day. In repeatedly infected animals, peak eosinophilia was observed during 3rd week of post infection and sustained upto 30th day with a slight decrease. These studies demonstrate that eosinophilia is induced due to antigen-antibody reactions in the host system and eosinophilia in helminthic infections is amnestic response.

Ancylostomiasis↗
PubMed1991

Effects of alcohol on natural resistance to Ancylostoma caninum in mice.

Experiments were performed to study the effect of alcohol on natural immunity of Swiss albino mice during Ancylostoma caninum infection. Natural resistance is lowered in alcoholic mice; 40% alcohol by mouth produced significant effect after 20 daily injections. The effect of the drug on natural immunity depended upon the concentration and the period of consumption. Animals retained maximum number of larvae during alcohol treatment.

Alcoholism↗
PubMed1991

Transaminases changes and worm burden in multiple infection of Ancylostoma caninum in mice.

Aspartate transaminase level was significantly increased in intestine of experimentals that received multiple doses of infection. The level of alanine transaminase rose to a significant value in liver of mice infected with multiple doses. Rise of transaminases is correlated with the necrosis (disruption of intestina) hepatic cells in infected mice. Changes and/or the distribution of aspartate transaminase and alanine transaminase with regard to the dosage is discussed.

Alanine Transaminase↗
PubMed1989

Enzymes activity and worm burden in intestine and liver of mice infected with single doses of Ancylostoma caninum larvae.

Transaminases increased significantly in the intestine and liver of mice infected with 500, 1,000 and 2,000 doses of Ancylostoma caninum larvae. The level of increase remained higher than those of controls throughout the experimental period. The rise of enzymes was mainly due to the pathological changes caused by the migrating larvae within the organs.

Alanine Transaminase↗
PubMed1989

Comparison of immune response in Swiss albino mice due to single and repeated doses of infective Ancylostoma caninum larvae after subcutaneous inoculation.

Migratory behaviour of Ancylostoma caninum larvae and host responses were studied in four groups of Swiss albino mice infected subcutaneously with single doses of 1,000, 2,000 and multiple doses of 500 + 500, 500 + 500 + 1,000. After the 1st day most of the larvae found in muscles in all the groups. There is no significant difference in the migratory behaviour and/or the adoptive pattern of larvae in both the singly and multiply infected animals.

Ancylostoma↗
PubMed1988

Serum enzymes and worm load in mice infected with repeated doses of Ancylostoma caninum larvae.

The alterations of serum enzymes induced in the host system of swiss albino mice by infection with Ancylostoma caninum larvae have been studied in multiple doses of infection. It was found that the level of transaminases, alkaline phosphatase and amylase increased significantly throughout the infection period when compared with controls. In infected animals, increase of enzymes was influenced by the host-parasite interactions caused due to the retained concentration of larval antigens.

Alanine Transaminase↗
PubMed1987

Changes in serum enzyme activities in mice infected with single doses of Ancylostoma caninum larvae.

Aspartate transaminase, alanine transaminase, alkaline phosphatase and amylase levels were significantly increased in experimental female Swiss albino mice infected with single doses of 500, 1,000 and 2,000 larvae of Ancylostoma caninum. Increase of enzyme levels is due to the leakage of large amounts of enzymes into the blood. The necrosis of cells during intestinal and extra intestinal phases of ancylostomiasis in mice is directly responsible for the increase of enzymes.

Alanine Transaminase↗
PubMed1987

Cell mediated immunity during experimental ancylostomiasis: a review.

Cell-mediated immunity is usually recognized only in a very small number of infections caused by helminths. The present review is engaged with some aspects of the cell-mediated immunity in infections caused by Ancylostoma caninum. Special attention is paid to the role of passive transfer of sensitized cells and serum. The paper is based on a series of own original investigations of the authors.

Ancylostoma↗
PubMed1986

Serum levels of aspartate transaminase, alanine transaminase and worm burden in mice infected with Ancylostoma caninum larvae.

Aspartate transaminase and alanine transaminase levels showed considerable increase during initial (1 to 9 days) period of infection in all the experimental groups of mice and their level reached higher values than in the controls. The increase of enzymes was influenced by the degree of retention of worm load and the immunological damage caused by Ancylostoma caninum larvae within the host system.

Alanine Transaminase↗
PubMed1983

Pattern of immune response in recipients after transfer of mesenteric lymph node cells sensitized with low and high doses of Ancylostoma caninum.

Cellular immune response was detected in female Swiss albino mice with mesenteric lymph node cells during Ancylostoma caninum infection. Sensitized lymphocytes responded vigorously to A. caninum antigens and it was found that the cells (singly or repeatedly) sensitized with either low or high doses could confer adoptive protective immunity in recipients later challenged with a single dose of 500 A. caninum larvae.

Ancylostoma↗
PubMed1983

Transfer of delayed hypersensitivity through repeatedly sensitized peritoneal exudate cells during experimental ancylostomiasis in mice.

Current investigations using sensitized peritoneal exudate cells from mice infected repeatedly with Ancylostoma caninum larvae supply the evidence that these cells have the capability to transfer passive immunity to isogeneic recipients in contrast to normal cells from uninfected donors. High doses of sensitization to donors and a lapse of time between the expansion and proliferation of cells in the recipients provide a rather strong immunological readiness to expel/destroy the worm burden. The larvae bore through the gut reaching the peritoneal cavity but cannot enter the liver and/or lungs; they are thus misled (in the absence of normal guiding influences) to muscles where they undergo allergic death.

Ancylostomiasis↗
PubMed1982

Experimental infection with Ancylostoma caninum larvae in mice. VII. Role of promethazine hydrochloride in the rejection of worm burden from the intestine.

Acquired resistance of female swiss albino mice (6 to 8 weeks old) to Ancylostoma caninum infection was demonstrated by the transfer of mesenteric lymph node cells (MLNC) from resistant (sensitised and promethazine treated), sensitised and untreated and non-sensitised and untreated donors to normal recipients of the same isogeneic strain. MLNC from sensitised and promethazine treated donors were more effective in suppressing the rejection mechanism of worm burden in recipients in comparison to the cells obtained from other donor groups; the drug may be exerting an inhibitory action at either the specific lymphoid level (reducing antibodies effect) and at the non-specific amine level.

Ancylostomiasis↗