Ultrastructure of alterations in rat submandibular gland after cutting the Ramus communicans n. lingualis cum tympani.
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Biomedical subjects
Publications and source records attributed to V Valeri.
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The duct of the swine sweat gland crosses the dermis and epidermis in sequence. The cells of the dermic segment seem to be related with cellular secretion and absorption. In the epidermic segment of the duct the whole morphology of the cells resembles the cellular morphology of the epidermic cells.
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The effect of electric stimulation with direct constant current in induced pseudarthrosis was investigated in the radius of adult dogs. Two surgical techniques for inducing nonunion in the lower third of the radius of adult dogs are also described. Results were evaluated by clinical, radiologic, and histologic examinations and by 99mTc scintillation. A total of 57 animals (41 electrically stimulated and 16 control animals) was used. Statistically significant data show activation of endochrondral ossification and bone union by electric stimulation.
A new stereological method was applied to estimate the average thickness of the trophoblastic layer in chorial villi from different regions of the human term placenta. Statistically significant differences were demonstrated among the 5 regions considered. The results suggest that the thickness of the trophoblastic layer is related to the pO2 gradient in the intervillous space blood.
The structure of the acini in the swine sweat gland is described here at the TEM level. The acini of the sweat gland in the pig is formed by 2 secretory cell types: dark seromucous cells and clear cells. The dark seromucous cells are actively secretory and their secretion is apocrine. The clear cells seem to be involved in an active transport of water and electrolytes through their cytoplasms.
The authors studied by means of sterological methods the volume density of syncytial sprouts in different cotyledonary regions of the human term placenta. The syncytial sprouts account for 4.92% of the placenta volume. Meanwhile, there were significant differences according to the site from which the sample was obtained. The lowest value (2.72%) corresponds to the central-parabasal zone of the cotyledon, close to the entry of the maternal blood into the intervillous space, whereas the highest values correspond to the "venous" regions. The results of the present study, as well as those reported by others, suggest that the lateral-subchorial zone would be the growing region of the cotyledon.
Syncytiotrophoblast from human term placenta, studied by stereological methods both by light and electron microscopy, was shown to account for 19.49% of the placenta volume. Significant differences in volume density were observed in syncytiotrophoblast from villi obtained from different regions of the cotyledon. These differences may be caused by the heterogeneous conditions of oxygenation in the intervillous space blood and its effect on trophoblastic growth. The volume density of the organelles, related to syncytioplasm volume, was 0.0524, 0.0122, 0.0033 and 0.1136, for mitochondria, lysosomes, Golgi complex and endoplasmic reticulum, respectively. There were no differences in the volume densities of the different organelles among the four cotyledonary regions studied.
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The effect of insulin (single subcutaneous injection 3 IU/100 g body weight) on the vaginal epithelium cells was studied in castrated adult female rats. At the electron-microscopic level, the vaginal cells showed development of the Golgi complexes and increase in the number and arrangement of ribosomes and secretory vesicles. The endometrial cells showed similar modifications, with stronger evidence of neoformation of polyribosomes. These results suggest that insulin stimulates the cellular activity of the vaginal and endometrial epithelia of castrated female rats.
A stereologic technique for estimating the average villi diameter was applied to study random samples of 12 human term placentas. The determined values were compared with those previously obtained by others with different techniques.
The effect of insulin (single subcutaneous injection, 3 IU/100 g body weight) on the vaginal epithelium cells of female adult castrated rats was studied. An increase in nuclear volume and modification of the cytological picture suggesting an activation of cellular metabolism were observed. This anabolic effect of insulin is observed in other cells of the organism, and is not confined to vaginal epithelium cells.
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