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Biomedical subjects

V Victoria-Troncoso

Publications and source records attributed to V Victoria-Troncoso.

At least 19 recordsLinked to original sources

Histoenzymologic study of hyalocytes in tissue culture.

The mitochondrias of the hyalocytes contain lactic dehydrogenase but no glucose-6-phosphate dehydrogenase, so that only aerobic respiration is possible. Among the lysosomal enzymes, acid phosphatases and beta-glucuronidase are found, the latter facilitating the turnover of the hyaluronic acid. There is no galactosidase, as the hyaluronic acid of the vitreous does not contain galactose.

Acid Phosphatase

[Importance of the viability of the endothelium of corneal grafts].

Since we use a very strict control of the viability of the endothelium of the graft, we have no more failures in corneal grafting not even in cases with bad prognoses, such as Fuchs' endothelial dystrophy. On the contrary, in corneal grafting without this control, we had failures in 20% of the cases.

Cell Survival

Histology of the epithelium of the normal and cataractous lens.

Compared with the normal lens, the cataractous lens is characterized by morphological and distribution anomalies of the epithelial cells. At the electron microscope an enlargement of the intercellular spaces and large intercellular, as well as intracellular, vacuoles are seen. An important development of the lysosomal system and of the endoplasmic reticulum is also observed.

Cataract

Corticosteroid glaucoma.

The non-depolymerised mucopolysaccharides of the anterior chamber angle can become hydrated. This biological oedema can obstruct the trabcculae, but the physiological liberation of catabolic enzymes from the lysosomes depolymerises the mucopolysaccharides and prevent this obstruction. This is, on the contrary, facilited by the corticosteroids, which reinforce the lysosomal membranes and impede the liberation of the catabolic enzymes. There are clones of goniocytes which are corticosensitive and other which are not. Depending upon the predominace of the one or the other, the cortisone test will be positive or negative.

Acid Phosphatase

Study of the lysosomes by vital stains in normal keratocytes and in keratocytes from macular dystrophy of the cornea.

The acridine orange is an excellent vital stain for the "in vivo" study of the lysosomal dynamics in cultured keratocytes. In normal keratocytes the lysosomes are mostly grouped around one or both nucleus poles. In keratocytes from macular dystrophy of the cornea there is a hypertrophy of the lysosomal system, which in vain tends to compensate for the consequences of the enzymatic deficiency.

Acridines

Immunology of the vitreous body.

The vitreous is feebly antigenic and is characterized by the absence of gamma-globulins and immunocompetent cells. Moreover, it is isolated from the aqueous humour by a mucopolysaccharide molecular filter. It represents an incomplete and primitive immunological system, reacting like an embryonic tissue and exhibiting only phagocytosis.

Animals

Neovascularization after argon laser photocoagulation of macular lesions.

In three patients treated for macular degeneration with argon laser photocoagulation choroidal neovascularization occurred four to six weeks later at the photocoagulation site. Two of the patients were treated for angioid streaks of the macular region, the third patient was treated for disciform macular degeneration. Argon laser photocoagulation also induced neovascularization experimentally in pigmented rabbits.

Angiography